Fermentative production of (R)-(−)-3-hydroxybutyrate using 3-hydroxybutyrate dehydrogenase null mutant of Ralstonia eutropha and recombinant Escherichia coli

2006 ◽  
Vol 102 (6) ◽  
pp. 529-534 ◽  
Author(s):  
Mari Shiraki ◽  
Takakazu Endo ◽  
Terumi Saito
Keyword(s):  
Escherichia Coli ◽  
Ralstonia Eutropha ◽  
Recombinant Escherichia Coli ◽  
Null Mutant ◽  
Fermentative Production

scholarly journals Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

2009 ◽  
Vol 76 (2) ◽  
pp. 622-626 ◽  
Author(s):  
Björn Andreeßen ◽  
Alvin Brian Lange ◽  
Horst Robenek ◽  
Alexander Steinbüchel
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Ralstonia Eutropha ◽  
Dry Weight ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase ◽  
Fed Batch ◽  
Interesting Application ◽  
Serovar Typhimurium ◽  
Fed Batch Fermentation

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

scholarly journals Fermentative production of 1-propanol from d-glucose, l-rhamnose and glycerol using recombinant Escherichia coli

2016 ◽  
Vol 122 (4) ◽  
pp. 421-426 ◽  
Author(s):  
Mitsuru Matsubara ◽  
Nobuyuki Urano ◽  
Shohei Yamada ◽  
Ai Narutaki ◽  
Misaki Fujii ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Fermentative Production
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