Polyhydroxyalkanoates Production by Recombinant Escherichia coli Harboring the Structural Genes of the Polyhydroxyalkanoate Synthases of Ralstonia eutropha and Pseudomonas aeruginosa Using Low Cost Substrate

2006 ◽  
Vol 6 (8) ◽  
pp. 1745-1750 ◽  
Author(s):  
Gustavo Graciano Fonseca ◽  
Regina Vasconcellos Antonio
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Ralstonia Eutropha ◽  
Low Cost ◽  
Recombinant Escherichia Coli ◽  
Structural Genes ◽  
Low Cost Substrate

scholarly journals Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Beatriz Pérez-Armendáriz ◽  
Carlos Cal-y-Mayor-Luna ◽  
Elie Girgis El-Kassis ◽  
Luis Daniel Ortega-Martínez
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Canola Oil ◽  
Low Cost ◽  
Rhamnolipid Production ◽  
Low Cost Substrate

scholarly journals Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

2020 ◽  
Author(s):  
Feng Zhao ◽  
Chao Guo ◽  
Qingfeng Cui
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Practical Significance ◽  
Glycerol Metabolism ◽  
Metabolic Intermediate ◽  
Spectra Analysis ◽  
Low Cost Substrate ◽  
Anaerobic Production

Abstract Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

scholarly journals Pseudomonas aeruginosa amidase: Aggregation in recombinant Escherichia coli

2011 ◽  
Vol 6 (7) ◽  
pp. 888-897 ◽  
Author(s):  
Patrícia Borges ◽  
Rita Pacheco ◽  
Amin Karmali
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Recombinant Escherichia Coli

scholarly journals Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

2021 ◽  
Author(s):  
Feng Zhao ◽  
Mengyao Zheng ◽  
Qingfeng Cui
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Practical Significance ◽  
Glycerol Metabolism ◽  
Metabolic Intermediate ◽  
Spectra Analysis ◽  
Low Cost Substrate ◽  
Anaerobic Production

Abstract Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

Photobiocatalytic H2 evolution of GaN:ZnO and [FeFe]-hydrogenase recombinant Escherichia coli

2020 ◽  
Vol 10 (12) ◽  
pp. 4042-4052 ◽  
Author(s):  
Nuttavut Kosem ◽  
Yuki Honda ◽  
Motonori Watanabe ◽  
Atsushi Takagaki ◽  
Zahra Pourmand Tehrani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Driving Force ◽  
Low Cost ◽  
Recombinant Escherichia Coli ◽  
H2 Evolution ◽  
Conversion Technologies

The need for sustainable, renewable and low-cost approaches is a driving force behind the development of solar-to-H2 conversion technologies.

Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases

2021 ◽  
pp. 105-115
Author(s):  
Clóvis Moreira ◽  
Gustavo Marçal Schmidt Garcia Moreira ◽  
Rafael Amaral Donassolo ◽  
Marcos Roberto Alves Ferreira ◽  
Mariliana Luiza Ferreira Alves ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Low Cost ◽  
Coli Cell ◽  
Recombinant Escherichia Coli ◽  
Cell Lysates

scholarly journals Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

2009 ◽  
Vol 76 (2) ◽  
pp. 622-626 ◽  
Author(s):  
Björn Andreeßen ◽  
Alvin Brian Lange ◽  
Horst Robenek ◽  
Alexander Steinbüchel
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Ralstonia Eutropha ◽  
Dry Weight ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase ◽  
Fed Batch ◽  
Interesting Application ◽  
Serovar Typhimurium ◽  
Fed Batch Fermentation

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

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