Generation of monoclonal antibodies using simplified single-cell reverse transcription-polymerase chain reaction and cell-free protein synthesis

Muhamad Ali; Kiyotaka Hitomi; Hideo Nakano

doi:10.1263/jbb.101.284

Generation of monoclonal antibodies using simplified single-cell reverse transcription-polymerase chain reaction and cell-free protein synthesis

Journal of Bioscience and Bioengineering ◽

10.1263/jbb.101.284 ◽

2006 ◽

Vol 101 (3) ◽

pp. 284-286 ◽

Cited By ~ 4

Author(s):

Muhamad Ali ◽

Kiyotaka Hitomi ◽

Hideo Nakano

Keyword(s):

Polymerase Chain Reaction ◽

Protein Synthesis ◽

Monoclonal Antibodies ◽

Single Cell ◽

Reverse Transcription ◽

Chain Reaction ◽

Free Protein ◽

Polymerase Chain ◽

Cell Free Protein Synthesis

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In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis

Journal of Bioscience and Bioengineering ◽

10.1016/j.jbiosc.2009.07.001 ◽

2010 ◽

Vol 109 (1) ◽

pp. 75-82 ◽

Cited By ~ 6

Author(s):

Yunita Sabrina ◽

Muhamad Ali ◽

Hideo Nakano

Keyword(s):

Protein Synthesis ◽

Monoclonal Antibodies ◽

Hepatitis B ◽

Single Cell ◽

Plasma Cell ◽

Rt Pcr ◽

Free Protein ◽

Cell Free Protein Synthesis ◽

Single Plasma

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Comparison of reverse transcription–quantitative polymerase chain reaction methods and platforms for single cell gene expression analysis

Analytical Biochemistry ◽

10.1016/j.ab.2012.05.010 ◽

2012 ◽

Vol 427 (2) ◽

pp. 178-186 ◽

Cited By ~ 14

Author(s):

Bridget C. Fox ◽

Alison S. Devonshire ◽

Marc-Olivier Baradez ◽

Damian Marshall ◽

Carole A. Foy

Keyword(s):

Gene Expression ◽

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Gene Expression Analysis ◽

Quantitative Polymerase Chain Reaction ◽

Chain Reaction ◽

Cell Gene Expression ◽

Polymerase Chain ◽

Cell Gene

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Enhancing the efficiency of cell-free protein synthesis through the polymerase-chain-reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues

Analytical Biochemistry ◽

10.1016/j.ab.2005.11.047 ◽

2006 ◽

Vol 351 (2) ◽

pp. 187-192 ◽

Cited By ~ 21

Author(s):

Jeong-Mi Son ◽

Jin-Ho Ahn ◽

Mi-Yeon Hwang ◽

Chang-Gil Park ◽

Cha-Yong Choi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Protein Synthesis ◽

Amino Acid ◽

Amino Acid Residues ◽

Chain Reaction ◽

Enhancer Sequence ◽

Extra Amino Acid ◽

Polymerase Chain ◽

Cell Free Protein Synthesis

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Massively Parallel Single-Molecule and Single-Cell Emulsion Reverse Transcription Polymerase Chain Reaction Using Agarose Droplet Microfluidics

Analytical Chemistry ◽

10.1021/ac2033084 ◽

2012 ◽

Vol 84 (8) ◽

pp. 3599-3606 ◽

Cited By ~ 93

Author(s):

Huifa Zhang ◽

Gareth Jenkins ◽

Yuan Zou ◽

Zhi Zhu ◽

Chaoyong James Yang

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Single Molecule ◽

Reverse Transcription ◽

Droplet Microfluidics ◽

Massively Parallel ◽

Chain Reaction ◽

Polymerase Chain

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Single cell reverse transcription-polymerase chain reaction (RT-PCR) and the GABA-A receptor

Neurochemistry International ◽

10.1016/0197-0186(95)00076-3 ◽

1996 ◽

Vol 28 (2) ◽

pp. 137-139 ◽

Cited By ~ 4

Author(s):

Sterling N. Sudweeks ◽

Roy E. Twyman

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Rt Pcr ◽

Chain Reaction ◽

Gaba A ◽

Polymerase Chain

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Global Single-Cell Reverse Transcription-Polymerase Chain Reaction (GSC RT-PCR)

Encyclopedic Dictionary of Genetics, Genomics and Proteomics ◽

10.1002/0471684228.egp05136 ◽

2004 ◽

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Rt Pcr ◽

Chain Reaction ◽

Polymerase Chain

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Single Cell Reverse Transcription-Polymerase Chain Reaction Analysis of Rat Supraoptic Magnocellular Neurons: Neuropeptide Phenotypes and High Voltage-Gated Calcium Channel Subtypes

Endocrinology ◽

10.1210/endo.140.11.7136 ◽

1999 ◽

Vol 140 (11) ◽

pp. 5391-5401 ◽

Cited By ~ 60

Author(s):

Eric Glasgow ◽

Kiyoshi Kusano ◽

Hemin Chin ◽

Êva Mezey ◽

W. Scott Young ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Calcium Channel ◽

Single Cell ◽

High Voltage ◽

Reverse Transcription ◽

Polymerase Chain Reaction Analysis ◽

Chain Reaction ◽

Magnocellular Neurons ◽

Voltage Gated ◽

Polymerase Chain

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Serotyping of human rotaviruses in the Tokyo area (1990–1993) by enzyme immununoassay with monoclonal antibodies and by reverse transcription and polymerase chain reaction amplification

Journal of Medical Virology ◽

10.1002/jmv.1890440208 ◽

1994 ◽

Vol 44 (2) ◽

pp. 162-165 ◽

Cited By ~ 17

Author(s):

Hiroshi Ushijima ◽

Atsushi Mukoyama ◽

Ayako Hasegawa ◽

Shuichi Nishimura ◽

Kyoko Konishi ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Monoclonal Antibodies ◽

Reverse Transcription ◽

Polymerase Chain Reaction Amplification ◽

Chain Reaction ◽

Reaction Amplification ◽

Polymerase Chain

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An Immunofluorescence-Assisted Microfluidic Single Cell Quantitative Reverse Transcription Polymerase Chain Reaction Analysis of Tumour Cells Separated from Blood

Journal of Circulating Biomarkers ◽

10.5772/61822 ◽

2015 ◽

Vol 4 ◽

pp. 11 ◽

Cited By ~ 3

Author(s):

Kazunori Hoshino ◽

HaeWon Chung ◽

Chun-Hsien Wu ◽

Kaarthik Rajendran ◽

Yu-Yen Huang ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Single Cell ◽

Reverse Transcription ◽

Polymerase Chain Reaction Analysis ◽

Tumour Cells ◽

Chain Reaction ◽

Polymerase Chain

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Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)52291-2 ◽

1991 ◽

Vol 266 (4) ◽

pp. 2632-2638 ◽

Cited By ~ 1

Author(s):

S A Lesley ◽

M A Brow ◽

R R Burgess

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Protein Synthesis ◽

Monoclonal Antibodies ◽

Transcription Factors ◽

Epitope Mapping ◽

Chain Reaction ◽

Polymerase Chain ◽

Vitro Protein

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