scholarly journals Effects of Delipidation and Oxygen Concentration on In Vitro Development of Porcine Embryos

2004 ◽  
Vol 50 (3) ◽  
pp. 287-295 ◽  
Author(s):  
Akihiro YONEDA ◽  
Keita SUZUKI ◽  
Tadashi MORI ◽  
Junji UEDA ◽  
Tomomasa WATANABE
Keyword(s):  
Oxygen Concentration ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos

Reproduction ◽  
1990 ◽  
Vol 89 (2) ◽  
pp. 573-578 ◽  
Author(s):  
J. G. E. Thompson ◽  
A. C. Simpson ◽  
P. A. Pugh ◽  
P. E. Donnelly ◽  
H. R. Tervit
Keyword(s):  
Oxygen Concentration ◽  
In Vitro Development ◽  
Effect Of Oxygen ◽  
Vitro Development

Interaction between embryos and culture conditions during in vitro development of bovine early embryos

Zygote ◽  
2008 ◽  
Vol 16 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Yoshikazu Nagao ◽  
Rumi Iijima ◽  
Kazuhiro Saeki
Keyword(s):  
Oxygen Concentration ◽  
Inorganic Phosphate ◽  
Drop Size ◽  
Culture Conditions ◽  
Cooperative Interaction ◽  
Blastocyst Development ◽  
In Vitro Development ◽  
Early Embryos ◽  
Vitro Development

SummaryVarious factors such as embryo density and substances in the medium can influence embryo development in vitro. These factors and the embryos probably interact with each other, however the interactions are not fully understood. To investigate the interactions, we examined the effects of the number of embryos, drop size, oxygen concentration and glucose and inorganic phosphate in the medium during protein-free culture of bovine IVM/IVF embryos. In Experiment 1, different numbers of embryos were cultured in a 50 μl drop of medium. The frequencies of blastocyst development in the groups with 25, 50 and 100 embryos per drop were higher than in the other groups. One, five and 25 embryos were cultured in different drop sizes (Experiment 2), a 50 µl drop of medium at different O2 concentrations (Experiment 3) and a 50 µl drop of medium excluding glucose and/or inorganic phosphate (Experiment 4). In Experiment 2, the size of the medium drops did not improve blastocyst development. In Experiment 3, the highest frequency of blastocyst development for one, five and 25 embryos per drop was obtained at 1, 2.5 and 5% O2, respectively. In Experiment 4, blastocyst development for one and five embryos per drop were improved in the medium excluded inorganic phosphate. These results indicate that there is a cooperative interaction among embryos during culture and that this interaction may be mediated by reduction of toxic factors in the medium. At low embryo density, reduced oxygen concentration or the exclusion of inorganic phosphate enhanced blastocyst development.

Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos.

1997 ◽  
Vol 75 (2) ◽  
pp. 483 ◽  
Author(s):  
Y Fujitani ◽  
K Kasai ◽  
S Ohtani ◽  
K Nishimura ◽  
M Yamada ◽  
...  
Keyword(s):  
Free Radicals ◽  
Oxygen Concentration ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Effect Of Oxygen ◽  
Vitro Development

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

2021 ◽  
pp. 106767
Author(s):  
Gizele A.L. Silva ◽  
Luana B. Araújo ◽  
Larissa C.R. Silva ◽  
Bruna B. Gouveia ◽  
Ricássio S. Barberino ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Phosphatidylinositol 3 Kinase ◽  
In Vitro Development ◽  
Vitro Development ◽  
Kinase Pathway ◽  
Phosphatidylinositol 3

scholarly journals Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets

2021 ◽  
Vol 22 (16) ◽  
pp. 8367
Author(s):  
Hien Lau ◽  
Shiri Li ◽  
Nicole Corrales ◽  
Samuel Rodriguez ◽  
Mohammadreza Mohammadi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Oxygen Consumption ◽  
Insulin Secretion ◽  
Oxygen Consumption Rate ◽  
Consumption Rate ◽  
In Vitro Development ◽  
Porcine Islets ◽  
Vitro Development

Pre-weaned porcine islets (PPIs) represent an unlimited source for islet transplantation but are functionally immature. We previously showed that necrostatin-1 (Nec-1) immediately after islet isolation enhanced the in vitro development of PPIs. Here, we examined the impact of Nec-1 on the in vivo function of PPIs after transplantation in diabetic mice. PPIs were isolated from pancreata of 8–15-day-old, pre-weaned pigs and cultured in media alone, or supplemented with Nec-1 (100 µM) on day 0 or on day 3 of culture (n = 5 for each group). On day 7, islet recovery, viability, oxygen consumption rate, insulin content, cellular composition, insulin secretion capacity, and transplant outcomes were evaluated. While islet viability and oxygen consumption rate remained high throughout 7-day tissue culture, Nec-1 supplementation on day 3 significantly improved islet recovery, insulin content, endocrine composition, GLUT2 expression, differentiation potential, proliferation capacity of endocrine cells, and insulin secretion. Adding Nec-1 on day 3 of tissue culture enhanced the islet recovery, proportion of delta cells, beta-cell differentiation and proliferation, and stimulation index. In vivo, this leads to shorter times to normoglycemia, better glycemic control, and higher circulating insulin. Our findings identify the novel time-dependent effects of Nec-1 supplementation on porcine islet quantity and quality prior to transplantation.

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