Effect of oxygen concentration and free radicals on in vitro development of in vitro-produced bovine embryos.

1997 ◽  
Vol 75 (2) ◽  
pp. 483 ◽  
Author(s):  
Y Fujitani ◽  
K Kasai ◽  
S Ohtani ◽  
K Nishimura ◽  
M Yamada ◽  
...  
Keyword(s):  
Free Radicals ◽  
Oxygen Concentration ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Effect Of Oxygen ◽  
Vitro Development

scholarly journals Effect of oxygen concentration on in-vitro development of preimplantation sheep and cattle embryos

Reproduction ◽  
1990 ◽  
Vol 89 (2) ◽  
pp. 573-578 ◽  
Author(s):  
J. G. E. Thompson ◽  
A. C. Simpson ◽  
P. A. Pugh ◽  
P. E. Donnelly ◽  
H. R. Tervit
Keyword(s):  
Oxygen Concentration ◽  
In Vitro Development ◽  
Effect Of Oxygen ◽  
Vitro Development

Transition of Cleavage Divisions During In Vitro Development of Bovine Embryos

2009 ◽  
Vol 26 (1) ◽  
pp. 42-47
Author(s):  
Hitoshi Ushijima ◽  
Kiyoshi Akiyama ◽  
Toshio Tajima
Keyword(s):  
Bovine Embryos ◽  
In Vitro Development ◽  
Vitro Development

Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells

2018 ◽  
Vol 54 (2) ◽  
pp. 289-299 ◽  
Author(s):  
Carolina Gonzales da Silva ◽  
Carlos Frederico Martins ◽  
Heidi Christina Bessler ◽  
Álvaro Moraes da Fonseca Neto ◽  
Tereza Cristina Cardoso ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Trichostatin A ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Vitro Development

scholarly journals Effect of Antioxidants Added to Culture Media on the in vitro Development of Bovine Embryos

2009 ◽  
Author(s):  
Mustafa Numan BUCAK ◽  
Muharrem SATILMIŞ ◽  
Sedat Hamdi KIZIL ◽  
Tahir KARAŞAHİN ◽  
Numan AKYOL
Keyword(s):  
Culture Media ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Vitro Development

In vitro development of the reconstructed bovine embryos activated at various time after electrofusion

2010 ◽  
Vol 37 (5) ◽  
pp. 446-452
Author(s):  
G. P. Malenko ◽  
A. V. Komissarov ◽  
O. I. Stepanov
Keyword(s):  
Bovine Embryos ◽  
In Vitro Development ◽  
Vitro Development

Effects of the HDAC inhibitor scriptaid on the in vitro development of bovine embryos and on imprinting gene expression levels

2018 ◽  
Vol 110 ◽  
pp. 79-85 ◽  
Author(s):  
R. Laguna-Barraza ◽  
M.J. Sánchez-Calabuig ◽  
A. Gutiérrez-Adán ◽  
D. Rizos ◽  
S. Pérez-Cerezales
Keyword(s):  
Gene Expression ◽  
Hdac Inhibitor ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Expression Levels ◽  
Imprinting Gene ◽  
Vitro Development ◽  
Gene Expression Levels

174 THE EFFECT OF DIFFERENT OVARY TRANSPORT TEMPERATURES ON IN VITRO DEVELOPMENT AND POST-THAW SURVIVAL OF BOVINE EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 203
Author(s):  
S. R. Cho ◽  
S. H. Choi ◽  
H. J. Kim ◽  
C. Y. Choe ◽  
H. J. Jin ◽  
...  
Keyword(s):  
Room Temperature ◽  
Development Rate ◽  
Control Group ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Embryo Freezing ◽  
Nuclear Maturation ◽  
Vitro Development ◽  
And Control

The present study was carried out to investigate the effect of different ovary transport temperatures on in vitro development and post-thaw survivability of bovine embryos. Bovine ovaries were collected at a local slaughterhouse and transported at 4 different temperature categories to the laboratory: 7–10�C (T1), 11–17�C (T2), 18–25�C (T3), and above 26�C (control group). The cumulus–oocyte complexes (COCs) were aspirated from 2–8 mm antral follicles using a syringe with an 18 gauge needle. Selected COCs were washed in HEPES-buffered tissue culture medium (TCM-199) supplemented with 5% FBS. Sets of 50 COCs were matured for 22 h in 4-well dishes of TCM-199 supplemented with 5% FBS, 10 �g mL-1 LH, and 10 �g mL-1 FSH, that had been previously covered with mineral oil and equilibrated in an atmosphere of 5% CO2 in air at 39�C. Mature COCs were fertilized with frozen–thawed semen treated with BO medium. To evaluate nuclear maturation to the metaphase II stage, the matured COCs were fixed in 1 : 3 acetic acid–ethanol for 30 s and stained with 3% basic Fuchsin. For embryo freezing, Day 7 and 8 blastocysts were equilibrated for 15 min in 1.8 M ethylene glycol as a cryoprotectant. Embryos were loaded into 0.25-mL straws at room temperature, plunged directly into a cooling chamber, kept at -7�C for 10 min, including time for seeding, and further cooled to -35�C at -0.3�C min-1; after 2 min at this temperature, they were plunged into liquid nitrogen. Thawing was performed by keeping straws at room temperature for 10 s, followed by immersion in a water bath at 37�C. The appearance of the embryos was evaluated immediately after warming and again at 24-h intervals for at least 3 days. The development rate was assessed by the re-expansion of the blastocoel and the hatching of blastocysts. Results were compared by ANOVA. The rates of maturation (to metaphase II), cleavage, and development to blastocysts were compared among treatment groups. Furthermore, frozen–thawed blastocysts were in vitro cultured to compare the survivability among groups. The maturation rates in the T1, T2, and T3 groups (24/40, 60.0%; 25/41, 61.0%; and 30/44, 68.2%, respectively) were significantly lower than that in the control group (36/44, 81.8%; P < 0.05). The cleavage rates in the T1 and T2 groups (61/116, 52.6% and 66/121, 54.5%) were significantly lower than that in the control group (112/134, 83.6%; P < 0.05). However, there was no difference in the development rate to blastocysts among all groups (27.9–33.0%; P > 0.05). The survivability of frozen–thawed embryos was significantly lower in the T1 group (6/13, 46.2%) than in the T2 (11/16, 68.8), T3 (13/18, 72.2%), and control groups (19/26, 73.1%; P < 0.05). In conclusion, the results suggest that ovary transport at 26�C may be optimal for better in vitro development and survival of frozen–thawed embryos produced in vitro. Furthermore, exposure of ovaries to temperatures below 10�C during transport may significantly decrease both in vitro development and survivability of frozen-thawed blastocysts.

159 THE EFFECT OF DIFFERENT ZWITTERIONIC BUFFERS AND PBS ON IN VITRO DEVELOPMENT AND MORPHOLOGY OF BOVINE EMBRYOS

2006 ◽  
Vol 18 (2) ◽  
pp. 187
Author(s):  
J. De la Fuente ◽  
A. Gutiérrez-Adán ◽  
P. Beltrán Breña ◽  
S. S. Pérez-Garnelo ◽  
A. T. Palasz
Keyword(s):  
Embryo Development ◽  
Cell Number ◽  
Apoptotic Cells ◽  
Bovine Embryos ◽  
Chi Square ◽  
Oh Groups ◽  
In Vitro Development ◽  
Chi Square Analysis ◽  
Vitro Development

It is assumed that, contrary to phosphate buffers, zwitterionic buffers are neutral. However, zwitterionic buffers containing hydroxymethyl or hydroxyethyl residues may interact with OH-groups in the media and produce formaldehyde (Shiraishi et al. 1993 Free Radic. Res. Commun. 19, 315-321). Also, it was shown that three zwitterionic buffers tested in this study interact with DNA (Stellwagen et al. 2000 Anal. Biochem. 287, 167-175). Our objective was to evaluate the effect of the following buffers: TES (T), MOPS (M), HEPES (H) (pKa values at 20�C: 7.2-7.5), and PBS on in vitro development and morphology of bovine embryos. Zwitterionic buffers and PBS were prepared at a concentration of 10 mM in TALP medium and the final pH was adjusted to 7.2. Bovine follicular fluid was aspirated from abattoir-derived ovaries and evenly divided into four tubes. Collected oocytes (five replicates) from each tube were processed separately through the entire IVM, IVF, and IVC procedures using washing medium buffered with: PBS (n = 490), Group 1; H (n = 438), Group 2; M (n = 440), Group 3; and T (n = 394), Group 4. All buffers contained 4 mg/mL BSA. Oocytes were matured in TCM-199 + 10% FCS and 10 ng/mL of epidermal growth factor and fertilized in Fert-TALP containing 25 mM bicarbonate, 22 mM sodium lactate, 1 mM sodium pyruvate, 6 mg/mL BSA-FAF, and 10 �g/mL heparin with 1 � 106 spermatozoa/mL. After 24 h, oocytes-sperm co-incubation presumptive zygotes were cultured in SOFaa medium with 8 mg/mL BSA at 39�C under paraffin oil and 5% CO2 in humidified air. Cumulus-oocyte complexes and zygotes were held in designated buffers ?16 min before oocyte maturation, ~7 min after IVM and before IVF, and ~18 min after IVF and before culture. The total time of oocyte/embryo exposure to each buffer was ?41 min. Embryo development was recorded on Days 4, 7, 8, and 9. A total of ten, Day 8 blastocysts were taken randomly from each treatment and fixed in 4% paraformaldehyde for total and apoptotic cells counts, and five blastocysts from each replicate and treatment were frozen for later mRNA analysis. Apoptosis were determined by TUNEL, using commercial In situ Cell Death Detection Kit (Roche Diagnostic, SL, Barcelono, Spain). Embryo development among groups was compared by chi-square analysis. The cleavage rates were not different among the groups: PBS, 70.8%; H, 76.5%; M, 77.5% and T, 73.6%. The number of embryos that developed to d8 cells at Day 4 was higher in M, 36.2%, and PBS, 37.6%, than in H, 30.6%, and T, 29.7%, but was not significantly different. However, more (P < 0.05) blastocysts developed at Days 7, 8, and 9 in H and M than in PBS and T groups (21.9% and 22.9% vs. 16.9% and 14.9%, respectively). No difference was found between groups in total cell number (98.8 � 7, PBS; 111.8 � 11.9, M; 106.8 � 12.9, H; and 104.3 � 9.7, T) and the number of apoptotic cells (9.2 � 1.0, P; 9.2 � 0.8, M; 12.9 � 1.8, H; and 9.7 � 0.9, T). Based on the results of this study, we conclude that within our protocol choice of buffer may affect embryo developmental rates but not morphology.

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