scholarly journals Involvement of interferon-tau in the induction of apoptotic, pyroptotic, and autophagic cell death-related signaling pathways in the bovine uterine endometrium during early pregnancy

2018 ◽  
Vol 64 (6) ◽  
pp. 495-502 ◽  
Author(s):  
Toshiyuki SUZUKI ◽  
Ryosuke SAKUMOTO ◽  
Ken-Go HAYASHI ◽  
Takatoshi OGISO ◽  
Hiroki KUNII ◽  
...  
Keyword(s):  
Cell Death ◽  
Signaling Pathways ◽  
Early Pregnancy ◽  
Autophagic Cell Death ◽  
Interferon Tau ◽  
Uterine Endometrium

scholarly journals The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow

1999 ◽  
Vol 160 (1) ◽  
pp. 21-33 ◽  
Author(s):  
RS Robinson ◽  
GE Mann ◽  
GE Lamming ◽  
DC Wathes
Keyword(s):  
Cross Sections ◽  
Early Pregnancy ◽  
In Situ Hybridisation ◽  
Progesterone Receptors ◽  
Prostaglandin F2alpha ◽  
Interferon Tau ◽  
Uterine Endometrium ◽  
Low Concentrations ◽  
Oxytocin Antagonist

The expression of oxytocin receptor (OTR) in the uterine endometrium plays an important role in the initiation of luteolysis. During early pregnancy, the conceptus secretes interferon tau (IFN|gt) which inhibits OTR up-regulation and luteolysis. In this study, uterine horn cross sections were collected on day 16 from 15 pregnant cows (PREG), 9 uninseminated controls and 5 inseminated cows with no embryo present. The latter two groups had similar results and were combined to form a single non-pregnant (NP) group. The animals were given an oxytocin challenge shortly before tissue collection to assess prostaglandin F2alpha (PGF2alpha) release through the measurement of the metabolite 13,14-dihydro-15-keto PGF2alpha (PGFM). The mRNAs for OTR, oestrogen receptor (ER) and progesterone receptor (PR) were localised by in situ hybridisation. The results were quantified by optical density (OD) measurements from autoradiographs using image analysis. OTR protein was measured by autoradiography with iodinated oxytocin antagonist and ER and PR protein was detected by immunocytochemistry. The release of PGFM after the oxytocin challenge was significantly higher in the 14 NP cows (187%+/-15%) compared with the PREG group (131%+/-11%) (P<0.01). Low concentrations of OTR mRNA were localised to the luminal epithelium (LE) in 6 out of the 14 NP cows, of which 2 also expressed OTR protein, while OTR mRNA and protein were undetectable in all the pregnant animals. These results indicated that the sampling time coincided with the onset of the luteolytic mechanism in the NP cows. On day 16 ER mRNA was detectable in both the LE and glands of both PREG and NP animals. There were no differences in either ER mRNA or protein between NP and PREG samples. PR mRNA was moderately expressed in the caruncular stroma, with lower levels in the dense caruncular-like stroma and glands. There were no differences between PREG and NP animals. The expression of PR mRNA and protein in the deep glands was variable between animals. These results suggested that, in cows, the presence of an embryo suppressed the expression of OTR, but had no effect on the expression of the transcriptionally regulated ER on day 16.

scholarly journals N-Adamantyl-4-methylthiazol-2-amine suppresses glutamate-induced autophagic cell death via PI3K/Akt/mTOR signaling pathways in cortical neurons

BMB Reports ◽  
2020 ◽  
Vol 53 (10) ◽  
pp. 527-532
Author(s):  
Seung-Ju Yang ◽  
A Reum Han ◽  
Hye-Rim Choi ◽  
Kyouk Hwang ◽  
Eun-A Kim ◽  
...  
Keyword(s):  
Cell Death ◽  
Signaling Pathways ◽  
Cortical Neurons ◽  
Mtor Signaling ◽  
Autophagic Cell Death

scholarly journals Progesterone and interferon tau-regulated genes in the ovine uterine endometrium: identification of periostin as a potential mediator of conceptus elongation

Reproduction ◽  
2009 ◽  
Vol 138 (5) ◽  
pp. 813-825 ◽  
Author(s):  
Hyo Won Ahn ◽  
Jennifer L Farmer ◽  
Fuller W Bazer ◽  
Thomas E Spencer
Keyword(s):  
Early Pregnancy ◽  
Calcium Binding ◽  
Collagen Type ◽  
Cdna Array ◽  
Interferon Tau ◽  
Uterine Endometrium ◽  
S100 Calcium Binding Protein ◽  
Trophectoderm Cells ◽  
Conceptus Development

During early pregnancy in ruminants, progesterone (P4) and interferon tau (IFNT) act on the endometrium to regulate genes hypothesized to be important for conceptus development and implantation. The present study was conducted to verify several candidate genes (actin α-2, smooth muscle, aorta (ACTA2), collagen, type III, α-1 (COL3A1), periostin (POSTN), secreted protein acidic cysteine-rich (SPARC), S100 calcium-binding protein A2 (S100A2),STAT5Aand transgelin (TAGLN)) regulated by pregnancy, P4, and/or IFNT in the endometrium determined using a custom ovine cDNA array.S100A2mRNA was detected primarily in endometrial epithelia and conceptuses.S100A2mRNA increased in endometrial epithelia from days 10 to 16 in cyclic ewes and from days 10 to 14 in pregnant ewes and declined thereafter. The abundance ofS100A2mRNA was less in endometrial luminal epithelium of IFNT-infused ewes receiving P4. Expression ofCOL3A1, SPARC, ACTA2, andTAGLNwas independent of pregnancy, P4, or IFNT.POSTNmRNA was detected primarily in compact stroma of intercaruncular and caruncular endometria, but not in the conceptus. EndometrialPOSTNmRNA increased between days 12 and 14 in pregnant but not cyclic ewes, andPOSTNmRNA was more abundant in uterine stroma of ewes treated with P4. POSTN protein was detected in uterine flushings of pregnant ewes and found to mediate attachment and stimulate migration of ovine trophectoderm cellsin vitro. These results support the ideas that POSTN and S100A2 are regulated by P4and IFNT respectively, and that POSTN is involved in conceptus elongation during early pregnancy.

Sorafenib induces autophagic cell death and apoptosis in hepatic stellate cell through the JNK and Akt signaling pathways

2016 ◽  
Vol 27 (3) ◽  
pp. 192-203 ◽  
Author(s):  
Huiyao Hao ◽  
Di Zhang ◽  
Junli Shi ◽  
Yan Wang ◽  
Lei Chen ◽  
...  
Keyword(s):  
Cell Death ◽  
Signaling Pathways ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Akt Signaling ◽  
Autophagic Cell Death
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