scholarly journals The Dynamin 2 inhibitor Dynasore affects the actin filament distribution during mouse early embryo development

2015 ◽  
Vol 61 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Qiao-Chu WANG ◽  
Jun LIU ◽  
Xing DUAN ◽  
Xiang-Shun CUI ◽  
Nam-Hyung KIM ◽  
...  
Keyword(s):  
Embryo Development ◽  
Actin Filament ◽  
Early Embryo ◽  
Early Embryo Development ◽  
Mouse Early Embryo ◽  
Dynamin 2

scholarly journals Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage

Cell Research ◽  
2002 ◽  
Vol 12 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Pan YONG ◽  
Zheng GU ◽  
Jin Ping LUO ◽  
Jun Ru WANG ◽  
Jia Ke TSO
Keyword(s):  
Embryo Development ◽  
Early Embryo ◽  
Cell Stage ◽  
Early Embryo Development ◽  
Mouse Early Embryo

scholarly journals The effect of various assisted hatching techniques on the mouse early embryo development

2014 ◽  
Vol 41 (2) ◽  
pp. 68 ◽  
Author(s):  
Sung Baek Park ◽  
Hye Jin Kim ◽  
Young Bae Choi ◽  
Kwang Hwa Ahn ◽  
Kee Hwan Lee ◽  
...  
Keyword(s):  
Embryo Development ◽  
Early Embryo ◽  
Assisted Hatching ◽  
Early Embryo Development ◽  
Mouse Early Embryo

scholarly journals SHP-1 Arrests Mouse Early Embryo Development through Downregulation of Nanog by Dephosphorylation of STAT3

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e86330 ◽  
Author(s):  
Songna Yin ◽  
Haibo Wu ◽  
Jiaxing Lv ◽  
Xinying Wu ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Embryo Development ◽  
Early Embryo ◽  
Early Embryo Development ◽  
Mouse Early Embryo

Minor Splicing Factors Zrsr1 and Zrsr2 Essential for Gametogenesis, Early Embryo Development and Conversion of Stem Cells into 2C-Like

2019 ◽  
Author(s):  
Isabel Gómez-Redondo ◽  
Priscila Ramos-Ibeas ◽  
Eva Pericuesta ◽  
Benjamín Planells ◽  
Raul Fernández-González ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryo Development ◽  
Early Embryo ◽  
Splicing Factors ◽  
Early Embryo Development

scholarly journals LIN28 coordinately promotes nucleolar/ribosomal functions and represses the 2C-like transcriptional program in pluripotent stem cells

2021 ◽  
Author(s):  
Zhen Sun ◽  
Hua Yu ◽  
Jing Zhao ◽  
Tianyu Tan ◽  
Hongru Pan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryo Development ◽  
Pluripotent Stem Cells ◽  
Rna Binding ◽  
Stem Cell Differentiation ◽  
Early Embryo ◽  
Transcriptional Program ◽  
Cell Stage ◽  
Early Embryo Development ◽  
Nucleolar Stress

AbstractLIN28 is an RNA binding protein with important roles in early embryo development, stem cell differentiation/reprogramming, tumorigenesis and metabolism. Previous studies have focused mainly on its role in the cytosol where it interacts with Let-7 microRNA precursors or mRNAs, and few have addressed LIN28’s role within the nucleus. Here, we show that LIN28 displays dynamic temporal and spatial expression during murine embryo development. Maternal LIN28 expression drops upon exit from the 2-cell stage, and zygotic LIN28 protein is induced at the forming nucleolus during 4-cell to blastocyst stage development, to become dominantly expressed in the cytosol after implantation. In cultured pluripotent stem cells (PSCs), loss of LIN28 led to nucleolar stress and activation of a 2-cell/4-cell-like transcriptional program characterized by the expression of endogenous retrovirus genes. Mechanistically, LIN28 binds to small nucleolar RNAs and rRNA to maintain nucleolar integrity, and its loss leads to nucleolar phase separation defects, ribosomal stress and activation of P53 which in turn binds to and activates 2C transcription factor Dux. LIN28 also resides in a complex containing the nucleolar factor Nucleolin (NCL) and the transcriptional repressor TRIM28, and LIN28 loss leads to reduced occupancy of the NCL/TRIM28 complex on the Dux and rDNA loci, and thus de-repressed Dux and reduced rRNA expression. Lin28 knockout cells with nucleolar stress are more likely to assume a slowly cycling, translationally inert and anabolically inactive state, which is a part of previously unappreciated 2C-like transcriptional program. These findings elucidate novel roles for nucleolar LIN28 in PSCs, and a new mechanism linking 2C program and nucleolar functions in PSCs and early embryo development.

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