scholarly journals The Effects of Administering Estradiol Benzoate Plus Progesterone During the Growth or Static Phases of the Dominant Follicle in CIDR-Treated Lactating Dairy Cows

2007 ◽  
Vol 53 (3) ◽  
pp. 591-596 ◽  
Author(s):  
Ui-Hyung KIM ◽  
Guk-Hyun SUH ◽  
Tai-Young HUR ◽  
Seog-Jin KANG ◽  
Hyun-Gu KANG ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Estradiol Benzoate ◽  
Dominant Follicle ◽  
Lactating Dairy Cows

235 FOLLICULAR WAVE EMERGENCE AND DEVELOPMENT FOLLOWING ESTRADIOL PLUS PROGESTERONE AT A GROWING OR A STATIC PHASE OF THE DOMINANT FOLLICLE IN CIDR-TREATED, LACTATING DAIRY COWS

2006 ◽  
Vol 18 (2) ◽  
pp. 225
Author(s):  
U. H. Kim ◽  
G. H. Suh ◽  
I. H. Kim
Keyword(s):  
Dairy Cows ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Exact Test ◽  
Follicular Wave ◽  
Lactating Dairy Cows ◽  
Array Transducer ◽  
Static Phase

Treatment with estradiol benzoate (EB), compared to GnRH, in a controlled internal drug release (CIDR)-based timed AI protocol induced relatively asynchronous follicular wave emergence, resulting in ovulation of smaller ovulating follicles (Kim et al. 2005 Theriogenology 63, 260-268). In this study, we compared new follicular wave emergence and development following treatment with EB (2 mg) plus progesterone (50 mg) at a growing (Day 3 after ovulation) or a static (Day 8) phase of the dominant follicle during the first follicular wave in CIDR-treated, lactating dairy cows. Previously synchronized, lactating dairy cows received CIDRs (InterAg, Hamilton, New Zealand; Day 0) 3 days after ovulation and were assigned to two treatment groups and a control group. Cows in the T1 group (n = 11) received 2 mg EB (SY Esrone; Samyang, Seoul, Korea) and 50 mg P4 (SY Ovaron; Samyang) i.m., and those in the control group (n = 10) received no treatment at that time. Other previously synchronized cows received a CIDR (Day 0) with injections of 2 mg EB plus 50 mg P4 i.m. 8 days after ovulation (T2 group, n = 10). Thereafter, all cows received PGF2� (Lutalyse; Pharmacia & Upjohn, Puurs, Belgium) at the time of CIDR removal on Day 7, and GnRH (Conceral; Dongbang Co., Seoul, Korea) on Day 9. Ovaries of each cow were examined by transrectal ultrasonography (Sonoace 600 with 7.5 MHz linear-array transducer; Medison Co., Ltd, Seoul, Korea) every 24 h from Days 0 to 9, on Day 11, and on Day 14 of treatment to observe changes in ovarian structures (follicles and corpora lutea (CL)). Data were analyzed by Fisher's exact test, Kruskal-Wallis test, and ANOVA using an SAS program (SAS Institute, Inc., Cary, NC, USA). Follicular wave emergence occurred within 7 days in 3/10 of the control group, 9/11 of the T1 group, and 9/10 of the T2 group (P < 0.05). The interval to wave emergence did not differ (P > 0.05) among groups (3.7 � 1.2, 4.8 � 0.5, 5.9 � 0.3 days, respectively). While the diameters of dominant follicles on Day 0 were greater (P < 0.01) in the T2 group (14.3 � 1.2 mm) than in the control (9.4 � 0.6 mm) and T1 (10.5 � 0.7 mm) group, diameters of preovulatory follicles on Day 9 were significantly smaller (P < 0.01) in the T1 (13.6 � 0.7 mm) and T2 (12.3 � 0.5 mm) groups than in the control group (16.4 � 0.8 mm). Synchronized ovulation by 40 h after the GnRH injection (Day 11) occurred in 10/10 of the control group, 10/11 of the T1 group, and 9/10 of the T2 group (P > 0.05). In conclusion, administration of 2 mg EB plus 50 mg P4 at both a growing and a static phase of the dominant follicle during the first follicular wave results in synchronous follicular wave emergence and development and also synchronized ovulation in CIDR-treated, lactating dairy cows.

scholarly journals Prostaglandin F2? as ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome

2021 ◽  
Vol 42 (1) ◽  
pp. 193-208
Author(s):  
Luiz Francisco Pfeifer ◽  
◽  
Natália Castro ◽  
Jamyle Pereira ◽  
Augusto Schneider ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Single Point ◽  
Estradiol Benzoate ◽  
Preovulatory Follicle ◽  
Chi Square ◽  
Lactating Dairy Cows ◽  
Chi Square Test ◽  
The Moment ◽  
Dichotomous Outcomes ◽  
To Receive

This study evaluated the effect of a prostaglandin F2? (PGF) analogue as an ovulatory stimulus in dairy cows and buffaloes raised in the Amazon biome. To this end, three experiments were performed in the state of Rondônia, located in the Amazon biome. In Experiment 1, 22 lactating dairy buffaloes received 2 mg of intramuscular (I.M.) estradiol benzoate (EB) on day 0 and an intravaginal progesterone-releasing device (CIDR) from day 0 to day 9 of the protocol. On days 8 and 9, all cows were given 500 ?g of I.M. d-cloprostenol (PGF analogue). On day 10, buffaloes were divided into two groups to receive 500 ?g of PGF (PGF group, n = 8) or no treatment (CTL group, n = 14), respectively. In Experiment 2, 16 lactating crossbred dairy cows (Holstein x Gir) received 2 mg of EB on day 0 and a CIDR insert from day 0 to day 8. On days 7 and 8, all cows were given 500 ?g of d-cloprostenol. On day 9, cows were divided into two groups to receive 500 ?g of d-cloprostenol (PGF group, n = 8) or no treatment (CTL group, n = 8), respectively. In Experiment 3, 16 lactating crossbred dairy cows (Holstein x Gir) were handled and treated similarly as in Experiment 2, although cows did not receive d-cloprostenol on day 8. Single-point outcome variables were analyzed using one-way analysis of variance (ANOVA), while proportions with dichotomous outcomes were analyzed with the chi-square test. In Experiment 1, there was no difference (P = 0.30) in the ovulation rate between groups, and, on average, 68% of the buffaloes ovulated. Moreover, the treatment did not affect the interval to ovulation (P = 0.61) nor the diameter of the preovulatory follicle (P = 0.47). As for Experiment 2, only one cow, from the PG group, did not ovulate. There were no differences between the CTL and PG groups (P = 0.69) in the moment of ovulation, which occurred in average 82 h after CIDR removal. Finally, in Experiment 3, cows treated with PGF ovulated earlier than those in the CTL group (62.5 ± 5.8 and 94.5 ± 13.5 h, respectively; P = 0.05). Collectively, these results suggest that PGF hastens ovulation in lactating dairy cows, whereas no effect was observed in dairy buffaloes.

13 Pre-synchronization by Induction of a Dominant Follicle Using a Progesterone Device in a GnRH-Based-Ovulation Synchronization Protocol in Lactating Dairy Cows

2018 ◽  
Vol 30 (1) ◽  
pp. 146
Author(s):  
M. P. Bottino ◽  
L. A. C. L. DaSilva ◽  
L. M. S. Simoes ◽  
G. Santos ◽  
I. Y. H. Martinez ◽  
...  
Keyword(s):  
Sustained Release ◽  
Dairy Cows ◽  
Prostaglandin F2α ◽  
Follicular Development ◽  
Experimental Period ◽  
Ovulation Rate ◽  
Dominant Follicle ◽  
Lactating Dairy Cows ◽  
Intravaginal Device ◽  
Synchronization Protocol

The objective was to compare a pre-synchronization protocol by induction of a dominant follicle using a progesterone intravaginal device before an Ovsynch protocol (Presynch) with Double-Ovsynch in lactating dairy cows. Lactating Bos indicus × Bos taurus crossbred cows (n = 440) were randomly allocated to 1 of 2 treatments (all IM injections): (1) Double-Ov (n = 228): GnRH (Day –17), prostaglandin F2α (PGF2α) 7 days later (Day –10) and gonadotropin-releasing hormone (GnRH) 3 days later (Day –7) followed by an Ovsynch protocol 7 days later (GnRH on Day 0, PGF2α on Day 7, GnRH on Day 9); (2) Presynch (n = 212): insertion of a sustained release progesterone intravaginal device (Day –10), 10 days later (Day 0), an Ovsynch protocol was initiated with progesterone device withdrawal on Day 7. All cows were artificially inseminated 15 to 20 h after the second GnRH injection of the Ovsynch protocol and were pregnancy checked by ultrasonography 30 and 60 days later. On a subsample (n = 102), ultrasonography was performed on Days 0, 7, 9, and 24 of the experimental period. On another subsample (n = 42), blood samples for progesterone analysis were taken on Days 0, 7, and 24. There were no differences between pre-synchronization methods on synchronization parameters [presence of a follicle >12 mm on D0, Double-Ov 94.2% (49/52) and Presynch 92.0% (46/50); P = 0.66], follicular diameter on the 1st GnRH (Double-Ov 17.2 ± 0.7 mm and Presynch 18.6 ± 0.9 mm; P = 0.28), ovulation rate to the 1st GnRH [Double-Ov 86.3% (44/51) and Presynch 81.2% (39/48); P = 0.50], synchronization rate [Double-Ov 84.6% (44/52) and Presynch 86.0% (43/50); P = 0.84], follicular diameter on the 2nd GnRH (Double-Ov 17.5 ± 0.6 mm and Presynch 18.0 ± 0.5 mm; P = 0.48), ovulation rate to the 2nd GnRH [Double-Ov 90.9% (40/44) and Presynch 86.0% (37/43); P = 0.48] and CL diameter on Day 24 (Double-Ov 27.9 ± 0.7 mm and Presynch 29.4 ± 0.9 mm; P = 0.19). Corpus luteum presence on Day 0 was different (P = 0.03) between treatments [Double-Ov 57.7% (30/52) and Presynch 36.0% (18/50)]. Moreover, there was no difference (P = 0.85) between pregnancy rates on Day 30 [Double-Ov 39.0% (89/228) and Presynch 40.1% (85/212)] or Day 60 after AI [Double-Ov 34.8% (79/227) and Presynch 38.7% (82/212); P = 0.41] and gestational loss between 30 and 60 days after AI [Double-Ov 7.9% (7/88) and Presynch 3.5% (3/85); P = 0.13]. Proportion of cows with P4 <1 ng mL−1 on Day 0 was similar between treatments [Double-Ov 13.6% (3/22) v. Presynch 5.0% (1/20); P = 0.37]. Likewise, the proportion of cows with P4 >1 ng mL−1 on Day 7 [Double-Ov 77.3% (17/22) v. Presynch 95.0% (19/20); P = 0.14] and P4 concentration on Day 24 (Double-Ov 4.7 ± 0.6 v. P4-Ov 5.9 ± 0.9; P = 0.84) were similar between treatments. In conclusion, pre-synchronization by induction of follicular persistence using a sustained-release progesterone device before Ovsynch yielded similar results to the Double-Ovsynch protocol on follicular development and regression patterns and on the fertility of lactating dairy cows.

Follicular dynamics, corpus luteum growth and regression in lactating dairy cattle

1991 ◽  
Vol 71 (1) ◽  
pp. 61-68 ◽  
Author(s):  
C. Taylor ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Estrous Cycle ◽  
Follicular Growth ◽  
Dominant Follicle ◽  
Lactating Dairy Cows ◽  
Ovulatory Follicle ◽  
Second Wave ◽  
Lactating Dairy Cattle ◽  
Follicular Dynamics

Ultrasound imaging and progesterone (P4) profiles were used to characterize follicular and corpus luteum (CL) growth and regression in 10 cycling dairy cows. Thirteen of sixteen cycles were characterized by two waves of follicular growth, the second wave giving rise to the ovulatory follicle. The remaining three cycles had three waves. There was no difference between two- and three-wave cycles with regard to the day of emergence of the first or second dominant follicle (day 2.5 ± 0.9 vs. day 2.4 ± 1.2 and day 12.4 ± 1.6 vs. day 12.0 ± 2.0, respectively) or the onset of regression of the first dominant follicle (day 12.5 ± 1.5 vs. day 12.3 ± 1.5). In two wave cycles, estrous cycle length was shorter (20.8 ± 1.7 d vs. 29.7 ± 4.9 d), the first day of decline in P4 (day 16.2 ± 1.5 vs. day 23.7 ± 5.5) and the onset of CL regression (day 17.1 ± 1.3 vs. day 24.3 ± 5.5) was earlier. It is concluded that two waves of follicular growth in an estrous cycle is the norm in lactating dairy cows. The presence of an additional wave is due to a prolonged luteal phase. Key words: Follicle, corpus luteum, estrous cycle, dairy cow

scholarly journals Prostaglandin F2α or estradiol benzoate to induce ovulation in timed artificially inseminated dairy cows

2016 ◽  
Vol 51 (6) ◽  
pp. 738-744 ◽  
Author(s):  
Luiz Francisco Machado Pfeifer ◽  
Luiz Gustavo Bruno Siqueira ◽  
Eduardo Kenji Nunes Arashiro ◽  
Natália Ávila de Castro ◽  
João Henrique Moreira Viana
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Crossbred Cows ◽  
Treatment Groups ◽  
Lactating Dairy Cows ◽  
Timed Artificial Insemination

Abstract: The objective of this work was to compare two types of ovulation inducers - estradiol benzoate (EB group) or prostaglandin F2α (PG group) -, in postpartum dairy cows subjected to timed artificial insemination (TAI). Lactating Holstein-Gir crossbred cows (n=118) with 60 to 120 days of milk production were used in this study. All cows were treated with an intravaginal progesterone-releasing device (CIDR) plus 2 mg of estradiol benzoate (EB) on day 0. The CIDR inserts were removed on day 9, and all cows received 500 µg sodium cloprostenol. Twenty-four hours after CIDR removal, cows were randomly assigned into two treatment groups and were given either 1 mg of EB i.m. (EB group) or 500 µg sodium cloprostenol i.m. (PG group). TAI were performed at 52 to 54 hours, in both EB and PG groups, after CIDR removal. No differences were observed in the ovulation (85.2% vs 72.7%) and pregnancy rates (40.7% vs 38.2%) between the EB and PG groups, respectively. Fertility is similar in the TAI protocols that use prostaglandin F2α or EB to induce ovulation in lactating dairy cows.

Managing the dominant follicle in lactating dairy cows

2011 ◽  
Vol 76 (9) ◽  
pp. 1568-1582 ◽  
Author(s):  
M.C. Wiltbank ◽  
R. Sartori ◽  
M.M. Herlihy ◽  
J.L.M. Vasconcelos ◽  
A.B. Nascimento ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Dominant Follicle ◽  
Lactating Dairy Cows
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