scholarly journals Assessment of Urinary Gonadotropin and Steroid Hormone Profiles of Female Cynomolgus Monkeys after Treatment with Pueraria mirifica

2007 ◽  
Vol 53 (2) ◽  
pp. 395-403 ◽  
Author(s):  
Hataitip TRISOMBOON ◽  
Suchinda MALAIVIJITNOND ◽  
Wichai CHERDSHEWASART ◽  
Gen WATANABE ◽  
Kazuyoshi TAYA
Keyword(s):  
Steroid Hormone ◽  
Pueraria Mirifica ◽  
Cynomolgus Monkeys

scholarly journals Histopathologic Characterization of Mifepristone-induced Ovarian Toxicity in Cynomolgus Monkeys

2018 ◽  
Vol 46 (3) ◽  
pp. 283-289 ◽  
Author(s):  
Yoshikazu Taketa ◽  
Kanta Horie ◽  
Tetsuya Goto ◽  
Etsuko Ohta ◽  
Kyoko Nakano-Ito ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Nonhuman Primates ◽  
Endometrial Thickness ◽  
Steroid Hormone ◽  
Antral Follicle ◽  
Sex Steroid ◽  
Sex Steroid Hormone ◽  
Cynomolgus Monkeys ◽  
Ovarian Toxicity ◽  
Precise Knowledge

Mifepristone, which is an orally active synthetic steroid with antiprogesterone activity, is known as an ovarian toxicant. Because the available data regarding the histopathologic characteristics of ovarian toxicity in nonhuman primates are limited, the present study was undertaken in order to investigate detailed histopathologic changes accompanying mifepristone-induced ovarian toxicity and its relationship to changes in menstrual cycle and circulating sex steroid hormone. Twenty mg/kg of mifepristone was orally administered daily to 4 cynomolgus monkeys for 2 months. Mifepristone inhibited the cyclic increases in circulating estradiol-17β and progesterone levels with associated absence of menstruation. Histopathologically, the ovary in the treated animals showed follicular phase without changes in the percentage of atretic antral follicles, and reduced endometrial thickness was noted in the uterus. These changes indicated that a certain degree of antral follicle development had been retained in spite of the menstrual cycle having been arrested in mifepristone-treated animals. Our investigation suggested that it is important to perform detailed histopathologic examination of reproductive organs with precise knowledge of the characteristics of each menstrual stage to detect ovarian toxicity in nonhuman primates. Monitoring menstrual signs and circulating sex steroid hormone levels provides additional evidence for the investigation of the mechanism of ovarian toxicity.

scholarly journals Long-Term Treatment Effects of Pueraria mirifica Phytoestrogens on Parathyroid Hormone and Calcium Levels in Aged Menopausal Cynomolgus Monkeys

2004 ◽  
Vol 50 (6) ◽  
pp. 639-645 ◽  
Author(s):  
Hataitip TRISOMBOON ◽  
Suchinda MALAIVIJITNOND ◽  
Juri SUZUKI ◽  
Yuzuru HAMADA ◽  
Gen WATANABE ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Treatment Effects ◽  
Term Treatment ◽  
Pueraria Mirifica ◽  
Cynomolgus Monkeys ◽  
Long Term Treatment

scholarly journals Effect of Pueraria mirifica on the Sexual Skin Coloration of Aged Menopausal Cynomolgus Monkeys

2006 ◽  
Vol 52 (4) ◽  
pp. 537-542 ◽  
Author(s):  
Hataitip TRISOMBOON ◽  
Suchinda MALAIVIJITNOND ◽  
Wichai CHERDSHEWASART ◽  
Gen WATANABE ◽  
Kazuyoshi TAYA
Keyword(s):  
Pueraria Mirifica ◽  
Cynomolgus Monkeys ◽  
Sexual Skin ◽  
Skin Coloration

scholarly journals Comparative Pharmacokinetics of Puerarin Alone and in Pueraria mirifica Extract in Female Cynomolgus Monkeys

Planta Medica ◽  
2020 ◽  
Author(s):  
Sureerat Namken ◽  
Phanit Songvut ◽  
Nitra Nuengchamnong ◽  
Taratorn Kemthong ◽  
Phisit Khemawoot ◽  
...  
Keyword(s):  
Normal Range ◽  
Pharmacological Activities ◽  
Traditional Use ◽  
Pueraria Mirifica ◽  
Cynomolgus Monkeys ◽  
Pharmacokinetic Profiles ◽  
Liquid Chromatography Tandem Mass ◽  
Absolute Oral Bioavailability ◽  
Human Use

Abstract Pueraria mirifica is an endemic Thai plant that has been used for rejuvenation and in the relief of various aging diseases. Puerarin is one of the major isoflavones found in this plant and shows several pharmacological activities in relation to the Thai traditional use of P. mirifica. Therefore, comparative pharmacokinetics of pure puerarin alone and that in a P. mirifica extract in cynomolgus monkeys were conducted in order to investigate the pharmacokinetic profiles of the 2 preparations. To this end, puerarin and P. mirifica extract, at an equivalent dose of 10 mg/kg of puerarin, were orally dosed to adult female monkeys for 7 consecutive days. A single intravenous injection of puerarin at a dose of 1 mg/kg was also peformed. Serial blood samples and excreta were collected from 0 – 24 h and 0 – 48 h after dosing. Determination of the puerarin levels and its metabolites in biological samples was conducted by liquid chromatography tandem mass spectrometry. Plasma levels of aspartate aminotransferase, alanine aminotransferase, and creatinine fluctuated in the normal range, with no abnormal physical signs in the animal. The absolute oral bioavailability of puerarin was approximately 1% in both preparations. Accumulation of puerarin was found after oral dosing for 7 consecutive days in both groups. Major metabolites of puerarin found in monkeys were hydroxylation and deglycosylation products. A negligible amount of unchanged puerarin was detected in urine and feces. Pharmacokinetic profiles obtained from this study could help to design the prescribed remedy of puerarin and P. mirifica extract phytopharmaceutical products for human use.

Hexachlorobenzene toxicity in the monkey ovary: III. Ultrastructure induced by high (10 mg/kg) dose exposure

1991 ◽  
Vol 49 ◽  
pp. 130-131
Author(s):  
A. Singh ◽  
A. Dykeman ◽  
J. Jarrelf ◽  
D. C. Villeneuve
Keyword(s):  
Present Report ◽  
Reproductive Toxicity ◽  
Control Group ◽  
Primate Model ◽  
Human Follicular Fluid ◽  
Thin Sections ◽  
Cynomolgus Monkeys ◽  
Cacodylate Buffer ◽  
Induction Cycle ◽  
Comprehensive Study

Hexachlorobenzene (HCB), a persistent and mobile organochlorine pesticide, occurs in environment. HCB has been shown to be present in human follicular fluid. An objective of the present report, which is part of a comprehensive study on reproductive toxicity of HCB, was to determine the cytologic effects of the compound on ovarian follicles in a primate model.Materials and Methods. Eight Cynomolgus monkeys were housed under controlled conditions at Animal facility of Health and Welfare, Ottawa. Animals were orally administered gelatin capsules containing HCB mixed with glucose in daily dosages of 0.0 or 10 mg/kg b.w. for 90 days; the former was the control group. On the menstrual period following completion of dosing, the monkeys underwent an induction cycle of superovulation. At necropsy, one-half of an ovary from each animal was diced into ca. 2- to 3-mm cubed specimens that were fixed by immersion in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3). Subsequent procedures followed to obtain thin sections that were examined in a Hitachi H-7000 electron microscope have been described earlier.

Hemodynamic effects of induced fetal anemia in cynomolgus monkeys

1998 ◽  
Vol 5 (1) ◽  
pp. 159A-159A
Author(s):  
W BLOCK ◽  
M OSULLIVAN ◽  
N SMITH ◽  
M DRAPER ◽  
C MIRABILE ◽  
...  
Keyword(s):  
Fetal Anemia ◽  
Hemodynamic Effects ◽  
Cynomolgus Monkeys
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