scholarly journals Evaluation of the spontaneous reversibility of carbon tetrachloride-induced liver cirrhosis in rabbits

2012 ◽  
Vol 46 (2) ◽  
pp. 122-128 ◽  
Author(s):  
E Bravo ◽  
E D'Amore ◽  
F Ciaffoni ◽  
C L Mammola
Keyword(s):  
Liver Cirrhosis ◽  
Carbon Tetrachloride ◽  
Treatment Interruption ◽  
Experimental Models ◽  
Morphometric Study ◽  
Postnecrotic Cirrhosis ◽  
Semiquantitative Evaluation ◽  
Hepatocyte Necrosis ◽  
And Control ◽  
Experimental Liver

There is a general consensus that liver fibrosis in humans is potentially reversible, while scepticism prevails on the concept that cirrhosis can be truly reversed. The availability of suitable experimental models is fundamental for disease research. The experimental murine model of liver cirrhosis induced by carbon tetrachloride (CCl4) reproduces both the histological picture of the postnecrotic cirrhosis and its biochemical and clinical parameters. Normal hepatic structure is modified by formation of regeneration nodules. Fibrosis represents a morphological element of disease and an effect of hepatocyte necrosis. However, the relevance for research of this well-established model of liver cirrhosis is hampered by some spontaneous cirrhosis regression reported in mice and rats. It has been reported that CCl4 also induces experimental liver cirrhosis in rabbits, but it is not known whether the process is reversible in this species. The aim of our study was to investigate this question. Male New Zealand White rabbits were treated intragastrically with CCl4 or the vehicle only for 19 weeks and groups were sacrificed three and five months after treatment interruption. Cirrhotic and control livers were processed for routine light microscopy and for morphometric study of fibrosis by semiquantitative evaluation. The degree of fibrosis was based on the Knodell's scoring system.

scholarly journals Review of experimental models for inducing hepatic cirrhosis by bile duct ligation and carbon tetrachloride injection

2012 ◽  
Vol 27 (8) ◽  
pp. 589-594 ◽  
Author(s):  
Thamirys Guimarães Marques ◽  
Eleazar Chaib ◽  
Juliana Hamati da Fonseca ◽  
Ana Cecília Rodrigues Lourenço ◽  
Felipe Duarte Silva ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Carbon Tetrachloride ◽  
Bile Duct ◽  
Web Sites ◽  
Bile Duct Ligation ◽  
Experimental Models ◽  
Duct Ligation ◽  
Experimental Liver Cirrhosis ◽  
Experimental Liver ◽  
Better Than

PURPOSE: To present a review about a comparative study of bile duct ligation versus carbon tetrachloride Injection for inducing experimental liver cirrhosis. METHODS: This research was made through Medline/PubMed and SciELO web sites looking for papers on the content "induction of liver cirrhosis in rats". We have found 107 articles but only 30 were selected from 2004 to 2011. RESULTS: The most common methods used for inducing liver cirrhosis in the rat were administration of carbon tetrachloride (CCl4) and bile duct ligation (BDL). CCl4 has induced cirrhosis from 36 hours to 18 weeks after injection and BDL from seven days to four weeks after surgery. CONCLUSION: For a safer inducing cirrhosis method BDL is better than CCl4 because of the absence of toxicity for researches and shorter time for achieving it.

scholarly journals Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride

JGH Open ◽  
2018 ◽  
Vol 2 (4) ◽  
pp. 117-123 ◽  
Author(s):  
Silvia Bona ◽  
Graziella Rodrigues ◽  
Andrea J Moreira ◽  
Fábio C Di Naso ◽  
Alexandre S Dias ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Carbon Tetrachloride ◽  
Experimental Liver Cirrhosis ◽  
Antifibrogenic Effect ◽  
Experimental Liver

Role of water channel AQP-CD in water retention in SIADH and cirrhotic rats

1995 ◽  
Vol 269 (6) ◽  
pp. F926-F931 ◽  
Author(s):  
N. Fujita ◽  
S. E. Ishikawa ◽  
S. Sasaki ◽  
G. Fujisawa ◽  
K. Fushimi ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Carbon Tetrachloride ◽  
Arginine Vasopressin ◽  
Water Retention ◽  
Collecting Duct ◽  
Water Channel ◽  
Experimental Models ◽  
Inappropriate Secretion ◽  
Observation Period

We determined whether aquaporin of collecting duct (AQP-CD) is involved in pathogenesis of water retention in rats with experimental models of syndrome of inappropriate secretion of antidiuretic hormone (SIADH) and liver cirrhosis. SIADH rats were made by administering 1-desamino-8-D-arginine vasopressin (DDAVP) subcutaneously and providing them with a liquid diet. Serum Na levels decreased to < 120 meq/l on day 2, and hyponatremia persisted throughout the rest of observation period. Six hours after the DDAVP infusion, the expression of AQP-CD mRNA significantly increased by 198%, followed by > 144% increases in its expression during the 14-day observation period. On day 7, the increased expression of AQP-CD mRNA was abolished after the administration of an antidiuretic, nonpeptide arginine vasopressin (AVP) antagonist, OPC-31260, which was closely related to a marked diuresis and a prompt normalization of serum Na levels in SIADH rats. Rats were made cirrhotic by injecting a mixture of carbon tetrachloride and olive oil subcutaneously for 3 mo. The expression of AQP-CD mRNA was increased by 164% in the decompensated cirrhotic rats. The blockade of AVP action by OPC-31260 significantly diminished its expression. These results indicate that water channel AQP-CD plays an important role in water retention in pathological states of SIADH and liver cirrhosis.

Protective and Therapeutic Effects of Huanglian-Jie-Du-Tang on Hepatotoxin-induced Liver Injuries

1996 ◽  
Vol 24 (03n04) ◽  
pp. 219-229 ◽  
Author(s):  
Song-Chow Lin ◽  
Chun-Ching Lin ◽  
Fung-Jou Lu ◽  
Yun-Ho Lin ◽  
Ching-Hsein Chen
Keyword(s):  
Carbon Tetrachloride ◽  
Experimental Models ◽  
Hepatoprotective Effect ◽  
Therapeutic Effects ◽  
Liver Injuries ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Hepatoprotective Effects ◽  
Experimental Liver ◽  
Serum Glutamate

The hepatoprotective effect of Huanglian-Jie-Du-Tang (HLJDT), a Chinese medicinal prescription, was investigated in three kinds of experimental models. The animals were treated with HLJDT (300 mg/kg, p.o.) thrice at 2, 4 and 10 hours after administration with carbon tetrachloride (32 μl/kg, i.p.), acetaminophen (600 mg/kg, i.p.) and β-D-galactosamine (188 mg/kg, i.p.). Significant hepatoprotective effects on carbon tetrachloride and actaminophen induced liver injuries were noted, but no significant effect on β-D-galactosamine induced liver injury was observed. These hepatoprotective effects were evidenced by comparing the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels in HLJDT treated and untreated groups. Serum enzyme activities in the carbon tetrachloride and acetaminophen experiments were significantly lower in the treated groups while the herbal prescription has no effect on the β-D-galactosamine experiment. These results demonstrated that Huanglian-Jie-Du-Tang has a hepatoprotective effect against experimental liver injuries induced by specific hepatotoxins, and therefore may be useful in treating some, but not all, liver injuries.

The efficacy of fp 736-04 in experimental liver cirrhosis

1998 ◽  
Vol 39 (5) ◽  
pp. 568-571
Author(s):  
A. Bergman ◽  
A. Magnusson ◽  
K. Moore ◽  
A. Sundin ◽  
S. Davies ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Experimental Liver Cirrhosis ◽  
Experimental Liver

Renal effects of nitric oxide synthesis inhibition in cirrhotic rats

1994 ◽  
Vol 267 (6) ◽  
pp. R1454-R1460 ◽  
Author(s):  
N. M. Atucha ◽  
J. Garcia-Estan ◽  
A. Ramirez ◽  
M. C. Perez ◽  
T. Quesada ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Renal Excretion ◽  
Renal Plasma Flow ◽  
Tubular Reabsorption ◽  
Nitric Oxide Synthesis ◽  
Important Mediator ◽  
Experimental Liver Cirrhosis ◽  
Endogenous Nitric Oxide ◽  
And Control ◽  
Experimental Liver

In the present study, we have characterized the renal response to inhibition of endogenous nitric oxide (NO) synthesis [intravenous NG-nitro-L-arginine methyl ester (L-NAME) for 3 h] in anesthetized cirrhotic rats, with (ASC) and without (CIR) ascites, at doses that do not change blood pressure (BP). Administration of L-NAME induced opposite effects on water (UV) and sodium (UNaV) excretion in cirrhotic and control animals. Infusion of 1 microgram.kg-1.min-1 of L-NAME in CIR (n = 5) decreased renal plasma flow (RPF) at the end of the 3-h period, whereas UV, UNaV, and glomerular filtration rate (GFR) were unaltered. In contrast, infusion of L-NAME at 10 micrograms.kg-1.min-1 in six more CIR increased UV and UNaV significantly by the 1st h, without changes in BP or GFR, and these parameters remained elevated throughout the experiment. Infusion of 1 microgram.kg-1.min-1 in ASC (n = 6) did not change BP or GFR but significantly enhanced UV and UNaV after the 1st h. These effects were prevented by pretreatment with L-arginine (0.1 mg.kg-1.min-1) in another group of ASC infused with 1 microgram.kg-1.min-1 of L-NAME. These results indicate that, in ASC and CIR cirrhotic rats, inhibition of NO synthesis at nonpressor does improves renal excretion of sodium and water via a decrease in tubular reabsorption. NO is an important mediator of the renal excretory and hemodynamic alterations of experimental liver cirrhosis.

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