Ultrasonographic detection and characterization of asymptomatic ductal carcinoma in situ with histopathologic correlation

2011 ◽  
Vol 52 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Yeon Ju Gwak ◽  
Hye Jung Kim ◽  
Jin Young Kwak ◽  
Sang Kwon Lee ◽  
Kyung Min Shin ◽  
...  
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma

scholarly journals Characterization of ductal carcinoma in situ cell lines established from breast tumor of a Singapore Chinese patient

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Jacklyn WY Yong ◽  
Meng Ling Choong ◽  
SiFang Wang ◽  
Yu Wang ◽  
Shermaine QY Lim ◽  
...  
Keyword(s):  
Cell Lines ◽  
Ductal Carcinoma In Situ ◽  
Breast Tumor ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Chinese Patient ◽  
Singapore Chinese

Characterization of ductal carcinoma in situ on diffusion weighted breast MRI

2011 ◽  
Vol 21 (9) ◽  
pp. 2011-2019 ◽  
Author(s):  
Habib Rahbar ◽  
Savannah C. Partridge ◽  
Peter R. Eby ◽  
Wendy B. DeMartini ◽  
Robert L. Gutierrez ◽  
...  
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Breast Mri ◽  
Diffusion Weighted

scholarly journals Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Julia Samson ◽  
Magdalina Derlipanska ◽  
Oza Zaheed ◽  
Kellie Dean
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Line ◽  
Cell Lines ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Anchorage Independent Growth

Abstract Background Currently it is unclear how in situ breast cancer progresses to invasive disease; therefore, a better understanding of the events that occur during the transition to invasive carcinoma is warranted. Here we have conducted a detailed molecular and cellular characterization of two, patient-derived, ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010. Methods Human DCIS cell lines, ETCC-006 and ETCC-010, were compared against a panel of cell lines including the immortalized, breast epithelial cell line, MCF10A, breast cancer cell lines, MCF7 and MDA-MB-231, and another DCIS line, MCF10DCIS.com. Cell morphology, hormone and HER2/ERBB2 receptor status, cell proliferation, survival, migration, anchorage-independent growth, indicators of EMT, cell signalling pathways and cell cycle proteins were examined using immunostaining, immunoblots, and quantitative, reverse transcriptase PCR (qRT-PCR), along with clonogenic, wound-closure and soft agar assays. RNA sequencing (RNAseq) was used to provide a transcriptomic profile. Results ETCC-006 and ETCC-010 cells displayed notable differences to another DCIS cell line, MCF10DCIS.com, in terms of morphology, steroid-receptor/HER status and markers of EMT. The ETCC cell lines lack ER/PR and HER, form colonies in clonogenic assays, have migratory capacity and are capable of anchorage-independent growth. Despite being isogenic, less than 30% of differentially expressed transcripts overlapped between the two lines, with enrichment in pathways involving receptor tyrosine kinases and DNA replication/cell cycle programs and in gene sets responsible for extracellular matrix organisation and ion transport. Conclusions For the first time, we provide a molecular and cellular characterization of two, patient-derived DCIS cell lines, ETCC-006 and ETCC-010, facilitating future investigations into the molecular basis of DCIS to invasive ductal carcinoma transition.

Characterization of High-grade Ductal Carcinoma In Situ With and Without Regressive Changes

2009 ◽  
Vol 17 (6) ◽  
pp. 495-499 ◽  
Author(s):  
Mamatha Chivukula ◽  
Akosua Domfeh ◽  
Gloria Carter ◽  
George Tseng ◽  
David J. Dabbs
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
High Grade ◽  
Regressive Changes

Histopathological characterization of ductal carcinoma in situ (DCIS) of the breast according to HER2 amplification status and molecular subtype

2014 ◽  
Vol 465 (3) ◽  
pp. 275-289 ◽  
Author(s):  
Mieke Van Bockstal ◽  
Kathleen Lambein ◽  
Hannelore Denys ◽  
Geert Braems ◽  
Ann Nuyts ◽  
...  
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Molecular Subtype ◽  
Her2 Amplification
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