A Tandem Time-of-Flight Mass Spectrometer: Combination of a Multi-Turn Time-of-Flight and a Quadratic Field Mirror

2005 ◽  
Vol 11 (2) ◽  
pp. 181-187 ◽  
Author(s):  
Michisato Toyoda ◽  
Anastassios E. Giannakopulos ◽  
Alex W. Colburn ◽  
Peter J. Derrick
Keyword(s):  
High Resolution ◽  
Mass Spectrometer ◽  
Mass Spectra ◽  
Quadratic Field ◽  
Time Of Flight ◽  
Tandem Mass ◽  
Unique Combination ◽  
Tandem Mass Spectra ◽  
Fragment Ions ◽  
Temporal Focus

A tandem time-of-flight (ToF) mass spectrometer consisting of a multi-turn ToF and a quadratic-field ion mirror has been designed and constructed. The instrument combines the unique capabilities of both ToF instruments, namely high-resolution and monoisotopic precursor ion selection from the multi-turn ToF and temporal focus for fragment ions with different kinetic energies from the quadratic-field mirror. The first tandem mass spectra for this unique combination of ToF systems are presented.

scholarly journals A Computational Framework for Heparan Sulfate Sequencing Using High-resolution Tandem Mass Spectra

2014 ◽  
Vol 13 (9) ◽  
pp. 2490-2502 ◽  
Author(s):  
Han Hu ◽  
Yu Huang ◽  
Yang Mao ◽  
Xiang Yu ◽  
Yongmei Xu ◽  
...  
Keyword(s):  
High Resolution ◽  
Heparan Sulfate ◽  
Mass Spectra ◽  
Tandem Mass ◽  
Computational Framework ◽  
Tandem Mass Spectra

scholarly journals De Novo Sequencing of Peptides from High-Resolution Bottom-Up Tandem Mass Spectra using Top-Down Intended Methods

PROTEOMICS ◽  
2017 ◽  
Vol 17 (23-24) ◽  
pp. 1600321 ◽  
Author(s):  
Kira Vyatkina ◽  
Lennard J. M. Dekker ◽  
Si Wu ◽  
Martijn M. VanDuijn ◽  
Xiaowen Liu ◽  
...  
Keyword(s):  
High Resolution ◽  
Mass Spectra ◽  
De Novo ◽  
De Novo Sequencing ◽  
Tandem Mass ◽  
Top Down ◽  
Bottom Up ◽  
Tandem Mass Spectra

Predicting Molecular Formulas of Fragment Ions with Isotope Patterns in Tandem Mass Spectra

2005 ◽  
Vol 2 (3) ◽  
pp. 217-230 ◽  
Author(s):  
Jingfen Zhang ◽  
Wen Gao ◽  
Jinjin Cai ◽  
Simin He ◽  
Rong Zeng ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Tandem Mass ◽  
Tandem Mass Spectra ◽  
Fragment Ions

Unexpected peaks in tandem mass spectra due to reaction of product ions with residual water in mass spectrometer collision cells

2014 ◽  
Vol 28 (23) ◽  
pp. 2645-2660 ◽  
Author(s):  
Pedatsur Neta ◽  
Mahnaz Farahani ◽  
Yamil Simón-Manso ◽  
Yuxue Liang ◽  
Xiaoyu Yang ◽  
...  
Keyword(s):  
Mass Spectrometer ◽  
Mass Spectra ◽  
Residual Water ◽  
Tandem Mass ◽  
Tandem Mass Spectra ◽  
Product Ions

pClean: An Algorithm To Preprocess High-Resolution Tandem Mass Spectra for Database Searching

2019 ◽  
Vol 18 (9) ◽  
pp. 3235-3244
Author(s):  
Yamei Deng ◽  
Zhe Ren ◽  
Qingfei Pan ◽  
Da Qi ◽  
Bo Wen ◽  
...  
Keyword(s):  
High Resolution ◽  
Mass Spectra ◽  
Database Searching ◽  
Tandem Mass ◽  
Tandem Mass Spectra

scholarly journals Features-Based Deisotoping Method for Tandem Mass Spectra

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
Zheng Yuan ◽  
Jinhong Shi ◽  
Wenjun Lin ◽  
Bolin Chen ◽  
Fang-Xiang Wu
Keyword(s):  
Mass Spectra ◽  
Protein Identification ◽  
Score Function ◽  
Tandem Mass ◽  
Isotopic Cluster ◽  
Tandem Mass Spectra ◽  
Fragment Ions ◽  
The Relationship ◽  
Cluster Graphs

For high-resolution tandem mass spectra, the determination of monoisotopic masses of fragment ions plays a key role in the subsequent peptide and protein identification. In this paper, we present a new algorithm for deisotoping the bottom-up spectra. Isotopic-cluster graphs are constructed to describe the relationship between all possible isotopic clusters. Based on the relationship in isotopic-cluster graphs, each possible isotopic cluster is assessed with a score function, which is built by combining nonintensity and intensity features of fragment ions. The non-intensity features are used to prevent fragment ions with low intensity from being removed. Dynamic programming is adopted to find the highest score path with the most reliable isotopic clusters. The experimental results have shown that the average Mascot scores and F-scores of identified peptides from spectra processed by our deisotoping method are greater than those by YADA and MS-Deconv software.

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