Age-Related Changes in the Lateral Lipid Distribution in a Human Lens Described by Mass Spectrometry Imaging

2015 ◽  
Vol 21 (3) ◽  
pp. 297-303 ◽  
Author(s):  
Jaroslav Pol ◽  
Helena Faltyskova ◽  
Lukas Krasny ◽  
Michael Volný ◽  
Ondrej Vlacil ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Human Lens ◽  
Lipid Distribution ◽  
Age Related ◽  
Age Related Changes

Age-Related Changes in Human Lens Crystallins Identified by HPLC and Mass Spectrometry

1998 ◽  
Vol 67 (1) ◽  
pp. 21-30 ◽  
Author(s):  
ZHIXIANG MA ◽  
STACY R.A. HANSON ◽  
KIRSTEN J. LAMPI ◽  
LARRY L. DAVID ◽  
DAVID L. SMITH ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Lens ◽  
Lens Crystallins ◽  
Age Related ◽  
Age Related Changes

Age-related Changes in Human Lens Crystallins Identified by Two-dimensional Electrophoresis and Mass Spectrometry

1998 ◽  
Vol 67 (1) ◽  
pp. 31-43 ◽  
Author(s):  
KIRSTEN J. LAMPI ◽  
ZHIXIANG MA ◽  
STACY R.A. HANSON ◽  
MISUYOSHI AZUMA ◽  
MARJORIE SHIH ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Lens ◽  
Two Dimensional ◽  
Lens Crystallins ◽  
Two Dimensional Electrophoresis ◽  
Age Related ◽  
Dimensional Electrophoresis ◽  
Age Related Changes

scholarly journals Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1473
Author(s):  
Belal I. Hanafy ◽  
Gareth W. V. Cave ◽  
Yvonne Barnett ◽  
Barbara K. Pierscionek
Keyword(s):  
Protein Glycation ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Oxide Nanoparticles ◽  
Lens Protein ◽  
Oxygen Species ◽  
Human Lens Epithelial Cells ◽  
Age Related ◽  
Age Related Changes

Cerium oxide nanoparticles (nanoceria) are generally known for their recyclable antioxidative properties making them an appealing biomaterial for protecting against physiological and pathological age-related changes that are caused by reactive oxygen species (ROS). Cataract is one such pathology that has been associated with oxidation and glycation of the lens proteins (crystallins) leading to aggregation and opacification. A novel coated nanoceria formulation has been previously shown to enter the human lens epithelial cells (HLECs) and protect them from oxidative stress induced by hydrogen peroxide (H2O2). In this work, the mechanism of nanoceria uptake in HLECs is studied and multiple anti-cataractogenic properties are assessed in vitro. Our results show that the nanoceria provide multiple beneficial actions to delay cataract progression by (1) acting as a catalase mimetic in cells with inhibited catalase, (2) improving reduced to oxidised glutathione ratio (GSH/GSSG) in HLECs, and (3) inhibiting the non-enzymatic glucose-induced glycation of the chaperone lens protein α-crystallin. Given the multifactorial nature of cataract progression, the varied actions of nanoceria render them promising candidates for potential non-surgical therapeutic treatment.

Age-related changes of alpha-crystallin aggregate in human lens

Amino Acids ◽  
2006 ◽  
Vol 32 (1) ◽  
pp. 87-94 ◽  
Author(s):  
N. Fujii ◽  
Y. Shimmyo ◽  
M. Sakai ◽  
Y. Sadakane ◽  
T. Nakamura ◽  
...  
Keyword(s):  
Human Lens ◽  
Alpha Crystallin ◽  
Age Related ◽  
Age Related Changes

scholarly journals Distribution of Glycerophospholipids in the Adult Human Lens

Biomolecules ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 156 ◽  
Author(s):  
Jo Seng ◽  
Jessica Nealon ◽  
Stephen Blanksby ◽  
Todd Mitchell
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Imaging Mass Spectrometry ◽  
Human Lens ◽  
Limited Data ◽  
Outer Cortex ◽  
Adult Human ◽  
Cell Age ◽  
Human Lenses ◽  
The Relationship

In humans, the age of fibre cells differs across the ocular lens, ranging from those formed before birth in the core of the lens to those formed just prior to death in the outer cortex. The distribution of glycerophospholipids in the adult human lens should reflect this range; however, limited data currently exists to confirm this hypothesis. Accordingly, this study aimed to determine the distribution of glycerophospholipids in adult human lens using mass spectrometry imaging. To achieve this, 20-µm thick slices of two human lenses, aged 51 and 67 were analysed by matrix-assisted laser desorption ionisation imaging mass spectrometry. The data clearly indicate that intact glycerophospholipids such as phosphatidylethanolamine, phosphatidylserine, and phosphatidic acid are mainly present in the outer cortex region, corresponding to the youngest fibre cells, while lyso-phosphatidylethanolamine, likely produced by the degradation of phosphatidylethanolamine, is present in the nucleus (older fibre cells). This study adds further evidence to the relationship between fibre cell age and glycerophospholipid composition.

Age-Related Changes of Human Lens Epithelial Cells in vivo

2001 ◽  
Vol 33 (6) ◽  
pp. 363-366 ◽  
Author(s):  
Hideaki Oharazawa ◽  
Nobuhiro Ibaraki ◽  
Hironori Matsui ◽  
Kunitoshi Ohara
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Age Related ◽  
Age Related Changes

Age-related changes of anisotropic properties of water self-diffusion in human lens: Diffusion anisotropy in lens with aging

2001 ◽  
Vol 21 (1) ◽  
pp. 89-95
Author(s):  
I. V. Nesmelova ◽  
D. Sh. Idiyatullin ◽  
Z. G. Kamalov ◽  
V. D. Fedotov ◽  
K. H. Mayo
Keyword(s):  
Human Lens ◽  
Diffusion Anisotropy ◽  
Anisotropic Properties ◽  
Self Diffusion ◽  
Age Related ◽  
Age Related Changes
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