Age-Related Changes in Normal and Cataractous Human Lens Crystallins, Separated by Fast-Performance Liquid Chromatography

1994 ◽  
Vol 26 (3) ◽  
pp. 149-157 ◽  
Author(s):  
P.C. Pereira ◽  
J.S. Ramalho ◽  
C.J. Faro ◽  
M.C. Mota
Keyword(s):  
Liquid Chromatography ◽  
Human Lens ◽  
Lens Crystallins ◽  
Age Related ◽  
Fast Performance Liquid Chromatography ◽  
Age Related Changes

Age-Related Changes in Human Lens Crystallins Identified by HPLC and Mass Spectrometry

1998 ◽  
Vol 67 (1) ◽  
pp. 21-30 ◽  
Author(s):  
ZHIXIANG MA ◽  
STACY R.A. HANSON ◽  
KIRSTEN J. LAMPI ◽  
LARRY L. DAVID ◽  
DAVID L. SMITH ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Lens ◽  
Lens Crystallins ◽  
Age Related ◽  
Age Related Changes

Spatial and temporal mapping of the age-related changes in human lens crystallins

1985 ◽  
Vol 41 (6) ◽  
pp. 745-758 ◽  
Author(s):  
M.J. McFall-Ngai ◽  
L.-L. Ding ◽  
L.J. Takemoto ◽  
J. Horwitz
Keyword(s):  
Human Lens ◽  
Lens Crystallins ◽  
Age Related ◽  
Age Related Changes

Age-related Changes in Human Lens Crystallins Identified by Two-dimensional Electrophoresis and Mass Spectrometry

1998 ◽  
Vol 67 (1) ◽  
pp. 31-43 ◽  
Author(s):  
KIRSTEN J. LAMPI ◽  
ZHIXIANG MA ◽  
STACY R.A. HANSON ◽  
MISUYOSHI AZUMA ◽  
MARJORIE SHIH ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Lens ◽  
Two Dimensional ◽  
Lens Crystallins ◽  
Two Dimensional Electrophoresis ◽  
Age Related ◽  
Dimensional Electrophoresis ◽  
Age Related Changes

scholarly journals Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1473
Author(s):  
Belal I. Hanafy ◽  
Gareth W. V. Cave ◽  
Yvonne Barnett ◽  
Barbara K. Pierscionek
Keyword(s):  
Protein Glycation ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Oxide Nanoparticles ◽  
Lens Protein ◽  
Oxygen Species ◽  
Human Lens Epithelial Cells ◽  
Age Related ◽  
Age Related Changes

Cerium oxide nanoparticles (nanoceria) are generally known for their recyclable antioxidative properties making them an appealing biomaterial for protecting against physiological and pathological age-related changes that are caused by reactive oxygen species (ROS). Cataract is one such pathology that has been associated with oxidation and glycation of the lens proteins (crystallins) leading to aggregation and opacification. A novel coated nanoceria formulation has been previously shown to enter the human lens epithelial cells (HLECs) and protect them from oxidative stress induced by hydrogen peroxide (H2O2). In this work, the mechanism of nanoceria uptake in HLECs is studied and multiple anti-cataractogenic properties are assessed in vitro. Our results show that the nanoceria provide multiple beneficial actions to delay cataract progression by (1) acting as a catalase mimetic in cells with inhibited catalase, (2) improving reduced to oxidised glutathione ratio (GSH/GSSG) in HLECs, and (3) inhibiting the non-enzymatic glucose-induced glycation of the chaperone lens protein α-crystallin. Given the multifactorial nature of cataract progression, the varied actions of nanoceria render them promising candidates for potential non-surgical therapeutic treatment.

Age-related changes of alpha-crystallin aggregate in human lens

Amino Acids ◽  
2006 ◽  
Vol 32 (1) ◽  
pp. 87-94 ◽  
Author(s):  
N. Fujii ◽  
Y. Shimmyo ◽  
M. Sakai ◽  
Y. Sadakane ◽  
T. Nakamura ◽  
...  
Keyword(s):  
Human Lens ◽  
Alpha Crystallin ◽  
Age Related ◽  
Age Related Changes

Age-Related Changes of Human Lens Epithelial Cells in vivo

2001 ◽  
Vol 33 (6) ◽  
pp. 363-366 ◽  
Author(s):  
Hideaki Oharazawa ◽  
Nobuhiro Ibaraki ◽  
Hironori Matsui ◽  
Kunitoshi Ohara
Keyword(s):  
Epithelial Cells ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Age Related ◽  
Age Related Changes

Age-related changes of anisotropic properties of water self-diffusion in human lens: Diffusion anisotropy in lens with aging

2001 ◽  
Vol 21 (1) ◽  
pp. 89-95
Author(s):  
I. V. Nesmelova ◽  
D. Sh. Idiyatullin ◽  
Z. G. Kamalov ◽  
V. D. Fedotov ◽  
K. H. Mayo
Keyword(s):  
Human Lens ◽  
Diffusion Anisotropy ◽  
Anisotropic Properties ◽  
Self Diffusion ◽  
Age Related ◽  
Age Related Changes

Age-related changes in protein conformation in bovine lens crystallins

1985 ◽  
Vol 40 (3) ◽  
pp. 461-469 ◽  
Author(s):  
J.N. Liang ◽  
S.K. Bose ◽  
B. Chakrabarti
Keyword(s):  
Protein Conformation ◽  
Lens Crystallins ◽  
Bovine Lens ◽  
Age Related ◽  
Age Related Changes
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