scholarly journals Metabolic Effects of Glibenclamide in Isolated Rat Hepatocytes in the Absence of Extracellular Ca2+

1995 ◽  
Vol 69 (2) ◽  
pp. 173-176
Author(s):  
Takahide Nomura ◽  
Masatsugu Ohtsuki ◽  
Toshiko Watanabe ◽  
Seiko Hasegawa ◽  
Shigeru Matsui ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Metabolic Effects ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Potentiation by ammonia of the metabolic effects of pent-4-enoate in isolated rat hepatocytes

1984 ◽  
Vol 224 (1) ◽  
pp. 263-267 ◽  
Author(s):  
F X Coudé ◽  
G Grimber ◽  
P Parvy ◽  
D Rabier ◽  
J Bardet
Keyword(s):  
Cellular Metabolism ◽  
Rat Hepatocytes ◽  
Metabolic Effects ◽  
Maximum Effect ◽  
Half Maximum ◽  
Isolated Rat Hepatocytes ◽  
Glucose Synthesis ◽  
Beta Hydroxybutyrate ◽  
Isolated Rat ◽  
Pyruvate Ratio

The metabolic effects of pent-4-enoate were studied in isolated rat hepatocytes; 1 mM-pent-4-enoate did not significantly inhibit gluconeogenesis from lactate, alanine and glycerol, but significantly decreased glucose synthesis from pyruvate. The addition of 1 mM-NH4Cl led to a drastic inhibition of glucose synthesis from all these substrates. In hepatocytes incubated with 10 mM-alanine and 1 mM-oleate, pent-4-enoate at 0.05-1 mM slightly inhibited glucose synthesis and ketogenesis. The addition of ammonia resulted in a dramatic potentiation of the metabolic effects of pent-4-enoate. Half-maximum effect of ammonia was observed at 0.2 mM concentration. Concomitant cellular concentrations of ATP and acetyl-CoA were also decreased by the addition of ammonia, as were lactate/pyruvate ratio and beta-hydroxybutyrate/acetoacetate ratio. These data suggest that ammonia seriously interferes with the cellular metabolism of pent-4-enoate and leads to a dramatic potentiation of its effects.

scholarly journals Effects of phorbol esters on α1-adrenergic-mediated and glucagon-mediated actions in isolated rat hepatocytes

1985 ◽  
Vol 228 (1) ◽  
pp. 277-280 ◽  
Author(s):  
J A García-Sáinz ◽  
F Mendlovic ◽  
M A Martínez-Olmedo
Keyword(s):  
Cyclic Amp ◽  
Dose Response ◽  
Response Curve ◽  
Phorbol Esters ◽  
Rat Hepatocytes ◽  
Metabolic Effects ◽  
Isolated Rat Hepatocytes ◽  
The Right ◽  
Isolated Rat ◽  
Stimulation Of

Phorbol 12-myristate 13-acetate (PMA) inhibited the stimulation of ureogenesis produced by adrenaline, but produced a minimal displacement to the right of the dose-response curve for glucagon. However, PMA diminished the accumulation of cyclic AMP induced by glucagon. Dissociation between the cyclic AMP concentrations and the metabolic effects induced by glucagon is evidenced in the presence of phorbol esters.

scholarly journals Lysosomal triacylglycerol lipase and lipolysis in isolated rat hepatocytes.

1979 ◽  
Vol 254 (18) ◽  
pp. 8841-8846
Author(s):  
L.J. Debeer ◽  
J. Thomas ◽  
P.J. De Schepper ◽  
G.P. Mannaerts
Keyword(s):  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Triacylglycerol Lipase ◽  
Isolated Rat
Sign in / Sign up

Export Citation Format

Share Document