scholarly journals Electron Microscopic Studies on the Inhibition of Degranulation of Rat Mast Cells by a Novel Anti-Allergic Agent, PTPC

1994 ◽  
Vol 66 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Tetsuo Ohashi ◽  
Akihiko Watanabe ◽  
Toshiaki Nishigaki ◽  
Hiroshi Azuma
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Rat Mast Cells

Immuno-electron microscopic localization of fibronectin on rat mast cells

1982 ◽  
Vol 38 (4) ◽  
pp. 495-496
Author(s):  
J. Sasaki ◽  
M. Imanaka ◽  
S. Watanabe ◽  
N. Otsuka ◽  
K. Sugiyama
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Localization ◽  
Rat Mast Cells ◽  
Electron Microscopic Localization

scholarly journals Conjugated avidin binds to mast cell granules.

1985 ◽  
Vol 33 (1) ◽  
pp. 27-32 ◽  
Author(s):  
M D Tharp ◽  
L L Seelig ◽  
R E Tigelaar ◽  
P R Bergstresser
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Horseradish Peroxidase ◽  
Staining Technique ◽  
Cellular Structures ◽  
Clinical Tool ◽  
Electron Microscopic ◽  
Simple Method ◽  
Human Mast Cells ◽  
Microscopic Studies

The glycoprotein, avidin, conjugated either to the enzyme horseradish peroxidase, or to the fluorochrome dyes, fluorescein or rhodamine, identifies the granules of mast cells in both tissues and cell suspensions. In the absence of prior fixation, mast cells were not identified with conjugated avidin; however, granules released from these cells were stained with this labeled glycoprotein. The specificity of avidin for mast cells was confirmed by the absence of conjugated avidin-positive cells in the skin of mice (S1/S1d) deficient in mature dermal mast cells. Electron microscopic studies confirmed that avidin binds specifically to individual mast cell granules rather than to other cellular structures. Rodent and human mast cells were readily stained with avidin conjugated to horseradish peroxidase or to either of the fluorochrome dyes. The conjugated avidin staining technique is a reliable and simple method for identifying rodent and human mast cells, one that is useful as both an investigative and a clinical tool.

Electron microscopic demonstration of metals in rat mast cells

1980 ◽  
Vol 66 (3) ◽  
pp. 293-300 ◽  
Author(s):  
G. Danscher ◽  
J. Obel ◽  
O. Thorlacius-Ussing
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Demonstration ◽  
Electron Microscopic Demonstration ◽  
Rat Mast Cells

scholarly journals T cell-dependent mast cell degranulation and release of serotonin in murine delayed-type hypersensitivity.

1980 ◽  
Vol 152 (5) ◽  
pp. 1358-1374 ◽  
Author(s):  
P W Askenase ◽  
S Bursztajn ◽  
M D Gershon ◽  
R K Gershon
Keyword(s):  
Mast Cell ◽  
T Cell ◽  
Mast Cells ◽  
Mast Cell Degranulation ◽  
Delayed Type Hypersensitivity ◽  
Surface Activation ◽  
Ultrastructural Examination ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Endothelial Gaps

We have previously suggested that the release of serotonin (5-hydroxytryptamine) (5-HT) by local tissue mast cells is required for the elicitation of delayed-type hypersensitivity (DTH) in mice. In the current study, light microscopic radioautographs from animals treated with [3H]5-HT indicated that local mast cells released 5-HT between 6 and 18 h during the evolution of DTH. Ultrastructural examination of mast cells revealed surface activation, indicated by extension of surface filopodia, and degranulation by fusion and exocytosis. Light and electron microscopic studies of the endothelium of postcapillary venules at sites of DTH revealed the development of gaps between adjacent cells. The development of gaps permitted extravasation of tracers that was abolished by depletion or antagonism of 5-HT. Thus mast cells degranulated and released 5-HT in DTH, and this 5-HT acted on local vessels. Recipients of nonadherent, non-immunoglobulin-bearing sensitized lymphocytes also demonstrated similar mast cell degranulation and the formation of endothelial gaps. This indicated that mast cell degranulation and 5-HT release in murine DTH were probably T cell dependent.

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