Characteristics of Mast Cells in Chediak-Higashi Mice: Light and Electron Microscopic Studies of Connective Tissue and Mucosal Mast Cells

Pathobiology ◽  
1983 ◽  
Vol 51 (3) ◽  
pp. 130-139
Author(s):  
Patricia K. Crowle ◽  
Dwight E. Phillips
Keyword(s):  
Mast Cells ◽  
Connective Tissue ◽  
Electron Microscopic ◽  
Mucosal Mast Cells ◽  
Microscopic Studies

Mast cell heterogeneity

1984 ◽  
Vol 62 (6) ◽  
pp. 734-737 ◽  
Author(s):  
F. Shanahan ◽  
J. A. Denburg ◽  
J. Bienenstock ◽  
A. D. Befus
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Connective Tissue ◽  
Regulatory Peptides ◽  
Cell Heterogeneity ◽  
Cell Secretion ◽  
Biochemical Basis ◽  
Mucosal Mast Cells ◽  
Mast Cell Heterogeneity

Increasing evidence for the existence of inter- and intra-species mast cell heterogeneity has expanded the potential biological role of this cell. Early studies suggesting that mast cells at mucosal sites differ morphologically and histochemically from connective tissue mast cells have been confirmed using isolated intestinal mucosal mast cells in the rat and more recently in man. These studies also established that mucosal mast cells are functionally distinct from connective tissue mast cells. Thus, mucosal and connective tissue mast cells differ in their responsiveness to a variety of mast cell secretagogues and antiallergic agents. Speculation about the therapeutic use of antiallergic drugs in disorders involving intestinal mast cells cannot, therefore, be based on extrapolation from studies of their effects on mast cells from other sites. Regulatory mechanisms for mast cell secretion may also be heterogeneous since mucosal mast cells differ from connective tissue mast cells in their response to a variety of physiologically occurring regulatory peptides. The development of techniques to purify isolated mast cell sub-populations will facilitate future analysis of the biochemical basis of the functional heterogeneity of mast cells.

Mutual Phenotypic Changes between Connective Tissue Type and Mucosal Mast Cells

1987 ◽  
Vol 82 (3-4) ◽  
pp. 244-248 ◽  
Author(s):  
Yukihiko Kitamura ◽  
Yuzuru Kanakura ◽  
Sanae Sonoda ◽  
Hidekazu Asai ◽  
Toru Nakano
Keyword(s):  
Mast Cells ◽  
Connective Tissue ◽  
Tissue Type ◽  
Phenotypic Changes ◽  
Mucosal Mast Cells

Zymogen Secreting Cells in the Hepatopancreatic Duct of Pagurus Bernhardus (Linnaeus, 1758) and Clibanarius Longitarsus (De Haan, 1849) (Anomura)

Crustaceana ◽  
1995 ◽  
Vol 68 (5) ◽  
pp. 616-628
Author(s):  
C. Manjulatha ◽  
D. Erri Babu
Keyword(s):  
Connective Tissue ◽  
Secretory Activity ◽  
Secretory Cells ◽  
Main Duct ◽  
Zymogen Granules ◽  
Electron Microscopic ◽  
Food Material ◽  
Acidic Polysaccharide ◽  
Microscopic Studies ◽  
Pagurus Bernhardus

AbstractHistological and histochemical observations reveal that there are two clusters of acidic polysaccharide secreting glands at the opening of the mouth into the oesophagus in Pagurus bernhardus and Clibanarius longitarsus. Below these there are structurally similar glands in the connective tissue of the oesophagus. The epithelial cells lining the hepatopancreatic main duct show secretory activity and they secrete acidophilic granular secretions, which are chemically similar to vertebrate pancreatic zymogen granules. Electron microscopic studies reveal the presence of secretory cells in the hepatopancreatic duct and the synthesis of zymogens within these cells. The time of release of these granular secretions synchronizes with the release of the food material into the midgut.

scholarly journals Electron Microscopic Studies on the Inhibition of Degranulation of Rat Mast Cells by a Novel Anti-Allergic Agent, PTPC

1994 ◽  
Vol 66 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Tetsuo Ohashi ◽  
Akihiko Watanabe ◽  
Toshiaki Nishigaki ◽  
Hiroshi Azuma
Keyword(s):  
Mast Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Rat Mast Cells

scholarly journals Conjugated avidin binds to mast cell granules.

1985 ◽  
Vol 33 (1) ◽  
pp. 27-32 ◽  
Author(s):  
M D Tharp ◽  
L L Seelig ◽  
R E Tigelaar ◽  
P R Bergstresser
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Horseradish Peroxidase ◽  
Staining Technique ◽  
Cellular Structures ◽  
Clinical Tool ◽  
Electron Microscopic ◽  
Simple Method ◽  
Human Mast Cells ◽  
Microscopic Studies

The glycoprotein, avidin, conjugated either to the enzyme horseradish peroxidase, or to the fluorochrome dyes, fluorescein or rhodamine, identifies the granules of mast cells in both tissues and cell suspensions. In the absence of prior fixation, mast cells were not identified with conjugated avidin; however, granules released from these cells were stained with this labeled glycoprotein. The specificity of avidin for mast cells was confirmed by the absence of conjugated avidin-positive cells in the skin of mice (S1/S1d) deficient in mature dermal mast cells. Electron microscopic studies confirmed that avidin binds specifically to individual mast cell granules rather than to other cellular structures. Rodent and human mast cells were readily stained with avidin conjugated to horseradish peroxidase or to either of the fluorochrome dyes. The conjugated avidin staining technique is a reliable and simple method for identifying rodent and human mast cells, one that is useful as both an investigative and a clinical tool.

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