scholarly journals Actions of a newly synthesized compound (711389-S) on various types of experimentally induced arrhythmias in mammalian species in situ.

1988 ◽  
Vol 46 (4) ◽  
pp. 359-372 ◽  
Author(s):  
Yoshihiro KADOWAKI ◽  
Tetsuro OHTA ◽  
Nobuo HOTOKEBUCHI ◽  
Sadatoshi KIMOTO ◽  
Masao HARUNA ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Experimentally Induced

scholarly journals Actions of a Newly Synthesized Compound (711389-S) on Various Types of Experimentally Induced Arrhythmias in Mammalian Species In Situ

1988 ◽  
Vol 46 (4) ◽  
pp. 359-372
Author(s):  
Yoshihiro KADOWAKI ◽  
Tetsuro OHTA ◽  
Nobuo HOTOKEBUCHI ◽  
Sadatoshi KIMOTO ◽  
Masao HARUNA ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Experimentally Induced

scholarly journals Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm

Open Biology ◽  
2015 ◽  
Vol 5 (8) ◽  
pp. 150080 ◽  
Author(s):  
Catherine E. Au ◽  
Louis Hermo ◽  
Elliot Byrne ◽  
Jeffrey Smirle ◽  
Ali Fazel ◽  
...  
Keyword(s):  
Membrane Trafficking ◽  
Glucose Transporter ◽  
Molecular Level ◽  
Mammalian Species ◽  
Cytoskeletal Proteins ◽  
Epididymal Sperm ◽  
Quantitative Electron Microscopy ◽  
Cytoplasmic Droplet ◽  
Germ Cell Differentiation

Discovered in 1909 by Retzius and described mainly by morphology, the cytoplasmic droplet of sperm (renamed here the Hermes body) is conserved among all mammalian species but largely undefined at the molecular level. Tandem mass spectrometry of the isolated Hermes body from rat epididymal sperm characterized 1511 proteins, 43 of which were localized to the structure in situ by light microscopy and two by quantitative electron microscopy localization. Glucose transporter 3 (GLUT-3) glycolytic enzymes, selected membrane traffic and cytoskeletal proteins were highly abundant and concentrated in the Hermes body. By electron microscope gold antibody labelling, the Golgi trafficking protein TMED7/p27 localized to unstacked flattened cisternae of the Hermes body, as did GLUT-3, the most abundant protein. Its biogenesis was deduced through the mapping of protein expression for all 43 proteins during male germ cell differentiation in the testis. It is at the terminal step 19 of spermiogenesis that the 43 characteristic proteins accumulated in the nascent Hermes body.

A rapid procedure for the isolation of C0t-1 DNA from plants

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Michael S. Zwick ◽  
Robert E. Hanson ◽  
M. Nurul Islam-Faridi ◽  
David M. Stelly ◽  
Rod A. Wing ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Dna ◽  
Copy Number ◽  
Genomic Dna ◽  
Mammalian Species ◽  
Repetitive Dnas ◽  
Rapid Procedure ◽  
Dna Elements ◽  
Repetitive Dna Elements

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or "blocking" of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.Key words: C0t-1 DNA, in situ hybridization, BACs, plants.

scholarly journals Identification of KiSS-1 Product Kisspeptin and Steroid-Sensitive Sexually Dimorphic Kisspeptin Neurons in Medaka (Oryzias latipes)

Endocrinology ◽  
2008 ◽  
Vol 149 (5) ◽  
pp. 2467-2476 ◽  
Author(s):  
Shinji Kanda ◽  
Yasuhisa Akazome ◽  
Takuya Matsunaga ◽  
Naoyuki Yamamoto ◽  
Shunji Yamada ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Sexually Dimorphic ◽  
Neuronal System ◽  
Anatomical Distribution ◽  
Neuronal Populations ◽  
Hypothalamic Nuclei ◽  
Steroid Sensitive ◽  
Neuronal Systems ◽  
The Brain

Recently, a novel physiologically active peptide, kisspeptin (metastin), has been reported to facilitate sexual maturation and ovulation by directly stimulating GnRH neurons in several mammalian species. Despite its importance in the neuroendocrine regulation of reproduction, kisspeptin neurons have only been studied in mammals, and there has been no report on the kisspeptin or kisspeptin neuronal systems in nonmammalian vertebrates. We used medaka for the initial identification of the KiSS-1 gene and the anatomical distribution of KiSS-1 mRNA expressing neurons (KiSS-1 neurons) in the brain of nonmammalian species. In situ hybridization for the medaka KiSS-1 gene cloned here proved that two kisspeptin neuronal populations are localized in the hypothalamic nuclei, the nucleus posterioris periventricularis and the nucleus ventral tuberis (NVT). Furthermore, NVT KiSS-1 neurons were sexually dimorphic in number (male neurons ≫ female neurons) under the breeding conditions. We also found that the number of KiSS-1 neurons in the NVT but not that in the nucleus posterioris periventricularis was positively regulated by ovarian estrogens. The fact that there were clear differences in the number of NVT KiSS-1 neurons between the fish under the breeding and nonbreeding conditions strongly suggests that the steroid-sensitive changes in the KiSS-1 mRNA expression in the NVT occur physiologically, according to the changes in the reproductive state. From the present results, we conclude that the medaka KiSS-1 neuronal system is involved in the central regulation of reproductive functions, and, given many experimental advantages, the medaka brain may serve as a good model system to study its physiology.

scholarly journals Behaviour and mortality of benthic crustaceans in response to experimentally induced hypoxia and anoxia in situ

2010 ◽  
Vol 414 ◽  
pp. 195-208 ◽  
Author(s):  
A Haselmair ◽  
M Stachowitsch ◽  
M Zuschin ◽  
B Riedel
Keyword(s):  
Experimentally Induced

scholarly journals Autosomal assignment of OTC in marsupials and monotremes: implications for the evolution of sex chromosomes

1987 ◽  
Vol 50 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Andrew H. Sinclair ◽  
Jacyln M. Wrigley ◽  
Jennifer A. Marshall Graves
Keyword(s):  
Sex Chromosomes ◽  
Mammalian Species ◽  
Cdna Probe ◽  
Ornithine Transcarbamylase ◽  
Gene Coding ◽  
Inactivation Mechanism ◽  
Marsupial Species ◽  
Gene Maps ◽  
Eutherian Evolution

SummaryThe OTC gene coding for ornithine transcarbamylase is sex linked and subject to X inactivation in humans and mice. We have used a rat cDNA probe to localize OTC by in situ hybridization in marsupials and monotremes. The gene maps to an autosomal site in two distantly related marsupial species and in one monotreme (the platypus); the first demonstration that a gene X-linked in one mammalian species may be autosomal in another. Since the conservation of the mammalian X is thought to be a consequence of its isolation by the inactivation mechanism, we propose that an autosomal or pseudoautosomal segment containing OTC has been recruited into the inactivated region of the X rather recently in eutherian evolution while it remained autosomal, or was translocated to an autosome, in metatherian and prototherian mammals.

scholarly journals A primary linkage map of the porcine genome reveals a low rate of genetic recombination.

Genetics ◽  
1994 ◽  
Vol 137 (4) ◽  
pp. 1089-1100 ◽  
Author(s):  
H Ellegren ◽  
B P Chowdhary ◽  
M Johansson ◽  
L Marklund ◽  
M Fredholm ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Recombination ◽  
Mammalian Species ◽  
Porcine Genome ◽  
Large White ◽  
European Wild Boar ◽  
Domestic Breed ◽  
Polymerase Chain ◽  
Map Data

Abstract A comprehensive genetic linkage map of the porcine genome has been developed by typing 128 genetic markers in a cross between the European Wild Boar and a domestic breed (Large White). The marker set includes 68 polymerase chain reaction-formatted microsatellites, 60 anchored reference markers informative for comparative mapping and 47 markers which have been physically assigned by in situ hybridization. Novel multipoint assignments are provided for 54 of the markers. The map covers about 1800 cM, and the average spacing between markers is 11 cM. We used the map data to estimate the genome size in pigs, thereby addressing the total recombination distance in a third mammalian species. A sex-average genome length of 1873 +/- 139 cM was obtained by comparing the recombinational and physical distances in defined regions of the genome. This is strikingly different from the length of the human genome (3800-4000 cM) and is more similar to the mouse estimate (1600 cM). The recombination rate in females was significantly higher than in males.

scholarly journals Foraminiferal survival after long-term in situ experimentally induced anoxia

2013 ◽  
Vol 10 (11) ◽  
pp. 7463-7480 ◽  
Author(s):  
D. Langlet ◽  
E. Geslin ◽  
C. Baal ◽  
E. Metzger ◽  
F. Lejzerowicz ◽  
...  
Keyword(s):  
Benthic Foraminifera ◽  
Initial Conditions ◽  
Standing Stock ◽  
Labile Organic Matter ◽  
Northern Adriatic ◽  
Benthic Chambers ◽  
Standing Stocks ◽  
Experimentally Induced

Abstract. Anoxia was successfully induced in four benthic chambers installed at 24 m depth on the northern Adriatic seafloor from 9 days to 10 months. To accurately determine whether benthic foraminifera can survive experimentally induced prolonged anoxia, the CellTrackerTM Green method was applied and calcareous and agglutinated foraminifera were analyzed. Numerous individuals were found living at all sampling times and at all sampling depths (to 5 cm), supported by a ribosomal RNA analysis that revealed that certain benthic foraminifera were active after 10 months of anoxia. The results show that benthic foraminifera can survive up to 10 months of anoxia with co-occurring hydrogen sulfides. However, foraminiferal standing stocks decrease with sampling time in an irregular manner. A large difference in standing stock between two cores sampled under initial conditions indicates the presence of a large spatial heterogeneity of the foraminiferal faunas. An unexpected increase in standing stocks after one month is tentatively interpreted as a reaction to increased food availability due to the massive mortality of infaunal macrofaunal organisms. After this, standing stocks decrease again in cores sampled after 2 months of anoxia to then attain a minimum in the cores sampled after 10 months. We speculate that the trend of overall decrease of standing stocks is not due to the adverse effects of anoxia and hydrogen sulfides but rather due to a continuous diminution of labile organic matter.

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