scholarly journals Studies on Responsiveness of Hepatoma Cells to Catecholamines III. Difference between the Receptor-Adenylate Cyclase Regulating Systems in AH130 Cells and Cultured Normal Rat Liver Cells

1986 ◽  
Vol 42 (2) ◽  
pp. 281-288 ◽  
Author(s):  
Fujiko SANAE ◽  
Takayuki MATSUNAGA ◽  
Kenichi MIYAMOTO ◽  
Ryozo KOSHIURA
Keyword(s):  
Adenylate Cyclase ◽  
Rat Liver ◽  
Hepatoma Cells ◽  
Liver Cells ◽  
Rat Liver Cells ◽  
Normal Rat

scholarly journals A METHOD FOR THE QUANTITATIVE ESTIMATION OF CYTOPLASMIC STRUCTURES

1962 ◽  
Vol 15 (3) ◽  
pp. 481-487 ◽  
Author(s):  
Alden V. Loud
Keyword(s):  
Endoplasmic Reticulum ◽  
Rat Liver ◽  
Electron Micrographs ◽  
Quantitative Estimation ◽  
Liver Cells ◽  
Sampling Procedure ◽  
Rat Liver Cells ◽  
Normal Rat ◽  
Cytoplasmic Structures

A sampling procedure and calculations are described by which electron micrographs of cytoplasmic structures may be quantitatively analyzed. The relative areas occupied by formed bodies and by the "membrane space," the remainder of the cytoplasm, are evaluated. A method for making a measurement of the quantity of endoplasmic reticulum or other membrane profiles is described. The technic and results are illustrated with normal rat liver cells.

Role of cell surface receptor mobility in concanavalin A-induced agglutination of Novikoff hepatoma and normal rat liver cells

1980 ◽  
Vol 42 (1) ◽  
pp. 169-182
Author(s):  
P. Mastromarino ◽  
G. Neri ◽  
A. Serra ◽  
E.F. Walborg
Keyword(s):  
Cell Surface ◽  
Rat Liver ◽  
Liver Cells ◽  
Cell Surface Receptor ◽  
Lateral Mobility ◽  
Con A ◽  
Novikoff Hepatoma ◽  
Surface Fluorescence ◽  
Rat Liver Cells ◽  
Normal Rat

The relationshio between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepatoma and normal rat liver cells was investigated. Novikoff cells, incubated with fluorescein-labelled Con A at 3 degrees C displayed uniform, ring-like surface fluorescence. Increasing the temperature of the cells to 37 degrees C caused capping of Con A receptors in approximately 65% of the cells, a phenomenon that could be prevented by prefixing the cells with glutaraldehyde. In spite of these variations in Con A-receptor distribution, Con A-induced agglutination was remarkably constant over a temperature range from 3 to 37 degrees C. In contrast to Novikoff cells, normal hepatocytes displayed a uniform, ringlike surface fluorescence at both 3 and 37 degrees C. No capping was observed. However, hepatocytes, similar to Novikoff cells, were agglutinable by low concentrations of Con A. These findings indicate that, in this model system, Con A-induced cytoagglutination is not dependent upon long-range lateral mobility of Con A receptors. The qualitative differences in the lateral mobility of cell-surface Con A receptors of normal and malignant rat liver cells may represent a marker for neoplastioc transformation during hepatocarcinogenesis, adaptation to growth in ascitic form, or progression of a tumour to a more malignant state.

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