scholarly journals Effect of Extract of Pomegranate (Punica granatum L.) on Glycated Protein-iron Chelate-induced Toxicity: An In Vitro Study on Human Umbilical-vein Endothelial Cells

2008 ◽  
Vol 54 (4) ◽  
pp. 441-449 ◽  
Author(s):  
Ikuo Nishigaki ◽  
Peramaiyan Rajendran ◽  
Ramachandran Venugopal ◽  
Ganapathy Ekambaram ◽  
Dhanapal Sakthisekaran ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Iron Chelate ◽  
Human Umbilical Vein ◽  
Glycated Protein ◽  
Umbilical Vein Endothelial Cells ◽  
Punica Granatum L

scholarly journals Effect of TNFα stimulation on expression of kidney risk inflammatory proteins in human umbilical vein endothelial cells cultured in hyperglycemia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zaipul I. Md Dom ◽  
Caterina Pipino ◽  
Bozena Krolewski ◽  
Kristina O’Neil ◽  
Eiichiro Satake ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Systemic Exposure ◽  
In Vitro Study ◽  
Human Umbilical Vein ◽  
Intracellular Fraction ◽  
Inflammatory Proteins ◽  
Umbilical Vein Endothelial Cells ◽  
Extracellular Fraction

AbstractWe recently identified a kidney risk inflammatory signature (KRIS), comprising 6 TNF receptors (including TNFR1 and TNFR2) and 11 inflammatory proteins. Elevated levels of these proteins in circulation were strongly associated with risk of the development of end-stage kidney disease (ESKD) during 10-year follow-up. It has been hypothesized that elevated levels of these proteins in circulation might reflect (be markers of) systemic exposure to TNFα. In this in vitro study, we examined intracellular and extracellular levels of these proteins in human umbilical vein endothelial cells (HUVECs) exposed to TNFα in the presence of hyperglycemia. KRIS proteins as well as 1300 other proteins were measured using the SOMAscan proteomics platform. Four KRIS proteins (including TNFR1) were down-regulated and only 1 protein (IL18R1) was up-regulated in the extracellular fraction of TNFα-stimulated HUVECs. In the intracellular fraction, one KRIS protein was down-regulated (CCL14) and 1 protein was up-regulated (IL18R1). The levels of other KRIS proteins were not affected by exposure to TNFα. HUVECs exposed to a hyperglycemic and inflammatory environment also showed significant up-regulation of a distinct set of 53 proteins (mainly in extracellular fraction). In our previous study, circulating levels of these proteins were not associated with progression to ESKD in diabetes.

Protective Effect of Fresh Apple Skin Extract on Human Umbilical Vein Endothelial Cells Against Glycated Protein-iron Chelate Induced Toxicity

2007 ◽  
Vol 2 (2) ◽  
pp. 34-42
Author(s):  
Ikuo Nishigaki ◽  
Balasubramanian Rajkapoor ◽  
Ramachandran Venugopal ◽  
Palaniyandi Senthilnat ◽  
Hao Qian Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Protective Effect ◽  
Umbilical Vein ◽  
Skin Extract ◽  
Iron Chelate ◽  
Human Umbilical Vein ◽  
Glycated Protein ◽  
Umbilical Vein Endothelial Cells

Cytoprotective role of astaxanthin against glycated protein/iron chelate-induced toxicity in human umbilical vein endothelial cells

2009 ◽  
Vol 24 (1) ◽  
pp. 54-59 ◽  
Author(s):  
Ikuo Nishigaki ◽  
Peramaiyan Rajendran ◽  
Ramachandran Venugopal ◽  
Gnapathy Ekambaram ◽  
Dhanapal Sakthisekaran ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Iron Chelate ◽  
Human Umbilical Vein ◽  
Glycated Protein ◽  
Umbilical Vein Endothelial Cells
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