scholarly journals Stimulation of Proteoglycan Release from Cultured Vascular Endothelial Cell Layers by Sodium Spirulan

2004 ◽  
Vol 50 (6) ◽  
pp. 654-659 ◽  
Author(s):  
Toshiyuki Kaji ◽  
Satomi Shimada ◽  
Chika Yamamoto ◽  
Yasuyuki Fujiwara ◽  
Jung-Bum Lee ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Cell Layers ◽  
Proteoglycan Release ◽  
Stimulation Of

scholarly journals Divergent effects of extracellular and intracellular alkalosis on Ca2+ entry pathways in vascular endothelial cells

1997 ◽  
Vol 323 (2) ◽  
pp. 567-573 ◽  
Author(s):  
Ichiro WAKABAYASHI ◽  
Klaus GROSCHNER
Keyword(s):  
Endothelial Cells ◽  
Membrane Potential ◽  
Endothelial Cell ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Free Solution ◽  
Store Depletion ◽  
Acute Elevation ◽  
Stimulation Of

Modulation by alkalosis of basal leak Ca2+ entry and store-depletion-induced Ca2+ entry was investigated in the vascular endothelial cell line ECV 304. Ca2+ entry was monitored as the increase in the intracellular free Ca2+ concentration ([Ca2+]i) induced by elevation of the extracellular Ca2+ concentration. When ECV 304 cells were challenged with 100 nM thapsigargin in nominally Ca2+-free solution, [Ca2+]i increased transiently, and the increase in [Ca2+]i during a subsequent cumulative elevation of extracellular Ca2+ (from nominally Ca2+-free up to 5 mM) was markedly enhanced compared with non-stimulated cells (i.e. basal Ca2+ leak). Prolonged elevation of the extracellular pH (pHo) from 7.4 to 7.9 did not affect resting [Ca2+]i or the thapsigargin-induced [Ca2+]i transient evoked in nominally Ca2+-free solution, but increased leak Ca2+ entry as well as store-depletion-activated Ca2+ entry significantly. Basal Ca2+ leak and store-depletion-activated Ca2+ entry were enhanced either by acute elevation of pHo from 7.4 to 7.9 or by chronic alkalosis (pHo = 7.9). Stimulation of Ca2+ entry by extracellular alkalosis was observed both in normal and in high extracellular K+ (110 mM) solution, suggesting that the effects of alkalosis are independent of membrane potential. The intracellular pH (pHi) increased slightly during both acute and chronic extracellular alkalosis (from 7.22±0.01 to 7.37±0.04 and 7.45±0.05 respectively). Elevation of pHi to 7.60±0.06 at constant pHo by administration of 20 mM NH4Cl failed to stimulate, and in fact inhibited, store-depletion-activated Ca2+ entry. Our results demonstrate that a decrease in the extracellular but not the intracellular proton concentration promotes both basal and stimulated Ca2+ entry into endothelial cells.

scholarly journals Zn(ii)2,9-dimethyl-1,10-phenanthroline stimulates cultured bovine aortic endothelial cell proliferation

RSC Advances ◽  
2020 ◽  
Vol 10 (69) ◽  
pp. 42327-42337
Author(s):  
Takehiro Nakamura ◽  
Eiko Yoshida ◽  
Takato Hara ◽  
Tomoya Fujie ◽  
Chika Yamamoto ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Endothelial Cell Proliferation ◽  
Bovine Aortic Endothelial Cell ◽  
Vascular Endothelial ◽  
Aortic Endothelial Cell ◽  
Stimulation Of ◽  
Aortic Endothelial

Stimulation of vascular endothelial cell proliferation by Zn-12 can be mediated by the ERK1/2 activation independently of the FGF-2-FGFR pathway. Additionally, there may be other pathways involved in the Zn-12 stimulation.

scholarly journals Inhibition of the Association of Proteoglycans with Cultured Vascular Endothelial Cell Layers by Calcium and Sodium Spirulan

2002 ◽  
Vol 48 (3) ◽  
pp. 250-255 ◽  
Author(s):  
Toshiyuki Kaji ◽  
Satomi Shimada ◽  
Chika Yamamoto ◽  
Yasuyuki Fujiwara ◽  
Jung-Bum Lee ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Cell Layers

Effect of angiotensin(1-7) on the expression of E-selectin induced by angiotensinII and its mechanism in vascular endothelial cell

2010 ◽  
Vol 34 (8) ◽  
pp. S71-S71
Author(s):  
Xiaohui Shen ◽  
Zhi‑Bin Wen ◽  
Na Li ◽  
Qingmei Cheng ◽  
Xiaofan He ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial
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