scholarly journals Establishment of a Rat Hepatic Cell Line, KanR2-XL8, for a Reporter Gene Assay of Aryl Hydrocarbon Receptor Ligands

2004 ◽  
Vol 50 (5) ◽  
pp. 530-536 ◽  
Author(s):  
Masashi Sekimoto ◽  
Miho Iwamoto ◽  
Shoji Miyajima ◽  
Kiyomitsu Nemoto ◽  
Masakuni Degawa
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Cell Line ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Hepatic Cell ◽  
Receptor Ligands ◽  
Aryl Hydrocarbon ◽  
Hepatic Cell Line ◽  
Gene Assay

scholarly journals Detection of Aryl Hydrocarbon Receptor Activation by Some Chemicals in Food Using a Reporter Gene Assay

Foods ◽  
2016 ◽  
Vol 5 (1) ◽  
pp. 15 ◽  
Author(s):  
Yoshiaki Amakura ◽  
Tomoaki Tsutsumi ◽  
Morio Yoshimura ◽  
Masafumi Nakamura ◽  
Hiroshi Handa ◽  
...  
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Reporter Gene ◽  
Receptor Activation ◽  
Reporter Gene Assay ◽  
Aryl Hydrocarbon ◽  
Gene Assay

scholarly journals Activation of the Aryl Hydrocarbon Receptor by Some Vegetable Constituents Determined Using in Vitro Reporter Gene Assay

2003 ◽  
Vol 26 (4) ◽  
pp. 532-539 ◽  
Author(s):  
Yoshiaki Amakura ◽  
Tomoaki Tsutsumi ◽  
Masafumi Nakamura ◽  
Hiroko Kitagawa ◽  
Junko Fujino ◽  
...  
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Aryl Hydrocarbon ◽  
Gene Assay

scholarly journals In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Alberto Celma ◽  
Geeta Mandava ◽  
Agneta Oskarsson ◽  
Juan Vicente Sancho ◽  
Lubertus Bijlsma ◽  
...  
Keyword(s):  
Water Quality ◽  
Reporter Gene ◽  
Biological Activities ◽  
Reporter Gene Assay ◽  
Water Bodies ◽  
Good Tool ◽  
Adverse Health Effects ◽  
Aryl Hydrocarbon ◽  
Gene Assay

Abstract Background Fresh water bodies represent less than 1% of overall amount of water on earth and ensuring their quality and sustainability is pivotal. Although several campaigns have been performed to monitor the occurrence of micropollutants by means of chemical analysis, this might not cover the whole set of chemicals present in the sample nor the potential toxic effects of mixtures of natural and anthropogenic chemicals. In this sense, by selecting relevant toxicity endpoints when performing in vitro bioanalysis, effect-based methodologies can be of help to perform a comprehensive assessment of water quality and reveal biological activities relevant to adverse health effects. However, no prior bioanalytical study was performed in wetland water samples from the Spanish Mediterranean coastline. Methods Eleven samples from relevant water bodies from the Spanish Mediterranean coastline were collected to monitor water quality on 8 toxicity endpoints. Aryl hydrocarbon receptor (AhR), androgenicity (AR+ and AR−), estrogenicity (ER+ and ER−), oxidative stress response (Nrf2) and vitamin D receptor (VDR+ and VDR−) reporter gene assays were evaluated. Results AhR was the reporter gene assay showing a more frequent response over the set of samples (activated by 9 out of 11 samples), with TCDD-eq in the range 7.7–22.2 pM. For AR, ER and VDR assays sporadic activations were observed. Moreover, no activity was observed on the Nrf2 reporter gene assay. Wastewater and street runaway streams from Valencia could be responsible for enhanced activities in one of the water inputs in the Natural Park ‘L’Albufera’. Conclusions Water quality of relevant wetlands from the Spanish Mediterranean coastline has been evaluated. The utilization of a panel of 5 different bioassays to cover for different toxicity endpoints has demonstrated to be a good tool to assess water quality.

scholarly journals Selective Activation of Zebrafish Estrogen Receptor Subtypes by Chemicals by Using Stable Reporter Gene Assay Developed in a Zebrafish Liver Cell Line

2011 ◽  
Vol 125 (2) ◽  
pp. 439-449 ◽  
Author(s):  
Anne Cosnefroy ◽  
François Brion ◽  
Emmanuelle Maillot-Maréchal ◽  
Jean-Marc Porcher ◽  
Farzad Pakdel ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Cell Line ◽  
Reporter Gene ◽  
Liver Cell ◽  
Reporter Gene Assay ◽  
Receptor Subtypes ◽  
Selective Activation ◽  
Liver Cell Line ◽  
Gene Assay ◽  
Zebrafish Liver

scholarly journals Recruitment of the NCoA/SRC-1/p160 Family of Transcriptional Coactivators by the Aryl Hydrocarbon Receptor/Aryl Hydrocarbon Receptor Nuclear Translocator Complex

2002 ◽  
Vol 22 (12) ◽  
pp. 4319-4333 ◽  
Author(s):  
Timothy V. Beischlag ◽  
Song Wang ◽  
David W. Rose ◽  
Joseph Torchia ◽  
Suzanne Reisz-Porszasz ◽  
...  
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Reporter Gene ◽  
Transcriptional Activation ◽  
Mammalian Cells ◽  
Luciferase Reporter ◽  
Pas Domain ◽  
Transactivation Domain ◽  
Aryl Hydrocarbon ◽  
Transcriptional Coactivators ◽  
Gene Assay

ABSTRACT The aryl hydrocarbon receptor complex heterodimeric transcription factor, comprising the basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS) domain aryl hydrocarbon receptor (AHR) and aryl hydrocarbon receptor nuclear translocator (ARNT) proteins, mediates the toxic effects of TCDD (2,3,7,8 tetrachlorodibenzo-p-dioxin). The molecular events underlying TCDD-inducible gene activation, beyond the activation of the AHRC, are poorly understood. The SRC-1/NCoA-1, NCoA-2/GRIP-1/TIF-2, and p/CIP/AIB/ACTR proteins have been shown to act as mediators of transcriptional activation. In this report, we demonstrate that SRC-1, NCoA-2, and p/CIP are capable of independently enhancing TCDD-dependent induction of a luciferase reporter gene by the AHR/ARNT dimer. Furthermore, injection of anti-SRC-1 or anti-p/CIP immunoglobulin G into mammalian cells abolishes the transcriptional activity of a TCDD-dependent reporter gene. We demonstrate by coimmunoprecipitation and by a reporter gene assay that SRC-1 and NCoA-2 but not p/CIP are capable of interacting with ARNT in vivo after transient transfection into mammalian cells, while AHR is capable of interacting with all three coactivators. We confirm the interactions of ARNT and AHR with SRC-1 with immunocytochemical techniques. Furthermore, SRC-1, NCoA-2, and p/CIP all associate with the CYP1A1 enhancer region in a TCDD-dependent fashion, as demonstrated by chromatin immunoprecipitation assays. We demonstrate by yeast two-hybrid, glutathione S-transferase pulldown, and mammalian reporter gene assays that ARNT requires its helix 2 domain but not its transactivation domain to interact with SRC-1. This indicates a novel mechanism of action for SRC-1. SRC-1 does not require its bHLH-PAS domain to interact with ARNT or AHR, but utilizes distinct domains proximal to its p300/CBP interaction domain. Taken together, these data support a role for the SRC family of transcriptional coactivators in TCDD-dependent gene regulation.

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