scholarly journals Peroxidase-Like Catalytic Activity of Aqueous- and Immobilized-Mn3+-octabromo-porphyrins on Ion-Exchange Resin Supplied as Mimetic of Horseradish Peroxidase

2008 ◽  
Vol 56 (9) ◽  
pp. 1364-1366 ◽  
Author(s):  
Youji Kitamura ◽  
Katsuya Mori ◽  
Makiko Yamamoto ◽  
Akira Nozaki ◽  
Madoka Saito ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Ion Exchange ◽  
Horseradish Peroxidase ◽  
Exchange Resin ◽  
Ion Exchange Resin

Effect of Ion Exchange Resin Functionality on Catalytic Activity and Leaching of Palladium Nanoparticles in Suzuki Cross-Coupling Reactions

ChemCatChem ◽  
2016 ◽  
Vol 9 (3) ◽  
pp. 451-457 ◽  
Author(s):  
Beau Van Vaerenbergh ◽  
Jeroen Lauwaert ◽  
Wim Bert ◽  
Joris W. Thybaut ◽  
Jeriffa De Clercq ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Ion Exchange ◽  
Palladium Nanoparticles ◽  
Exchange Resin ◽  
Cross Coupling ◽  
Ion Exchange Resin ◽  
Coupling Reactions ◽  
Cross Coupling Reactions

scholarly journals INVESTIGATION OF REGULARITY OF USING IMMOBILIZED PEROXIDASE FOR OXIDATION OF PHENOL AND ITS DERIVATIVES

2020 ◽  
pp. 14-22
Author(s):  
Борис Борисович Тихонов ◽  
Полина Юрьевна Стадольникова ◽  
Александр Иванович Сидоров ◽  
Валентина Геннадьевна Матвеева
Keyword(s):  
Titanium Dioxide ◽  
Ion Exchange ◽  
Horseradish Peroxidase ◽  
Sodium Alginate ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Oxidation Reactions ◽  
Main Regularity ◽  
Oxidation Of Phenol

В статье проведен синтез и исследование активности и стабильности биокатализаторов на основе пероксидазы хрена, иммобилизованной на различных носителях - ионообменной смоле Amberlite IR-120, диоксиде титана и микросферах из альгината натрия. Доказана эффективность синтезированных биокатализаторов в реакциях окисления фенола и 4-хлорфенола в концентрациях до 2 ммоль/л. Выявлены основные закономерности, позволяющие максимально повысить эффективность иммобилизации пероксидазы хрена на исследованных носителях. In the article the synthesis and investigation of activity and stability of biocatalysts based on horseradish peroxidase immobilized on various carriers - ion-exchange resin Amberlite IR-120, titanium dioxide and microspheres from sodium alginate were carried out. The effectiveness of synthesized biocatalysts in the oxidation reactions of phenol and 4-chlorophenol in concentrations up to 2 mmol/l has been proven. The main regularity allowing to maximize efficiency of horseradish peroxidase immobilization on the investigated carriers are revealed.

The catalytic evaluation of bimetallic Pd-based nanocatalysts supported on ion exchange resin in nitro and alkyne reduction reactions

2019 ◽  
Vol 43 (18) ◽  
pp. 7083-7092 ◽  
Author(s):  
Tábata R. Silva ◽  
Daniela C. de Oliveira ◽  
Tarasankar Pal ◽  
Josiel B. Domingos
Keyword(s):  
Catalytic Activity ◽  
Ion Exchange ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Reduction Reactions ◽  
Simple Protocol ◽  
Opening Up

A simple protocol to produce bimetallic Pd-based nanocatalysts supported on Amberlite resin is presented. Superior catalytic activity and selectivity in nitro and alkyne reduction reactions was achieved, opening up new possibilities in the development of cheaper and selective catalysts for these important industrial reactions.

OPTIMIZATION OF DNA EXTRACTION METHODS FROM BIOLOGICAL MATERIALS OF HONEY BEES IN A DIFFERENT METAMOTPHOSIS PHASE

2016 ◽  
Vol 52 ◽  
pp. 171-176
Author(s):  
M. Palkina ◽  
O. Metlitska
Keyword(s):  
Ion Exchange ◽  
Dna Extraction ◽  
Honey Bees ◽  
Biological Material ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Extraction Methods ◽  
Chelex 100 ◽  
Drone Brood ◽  
Dna Extraction Methods

The aim of the research – adaptation, optimization and using of existing DNA extraction methods from bees’ biological material with the reagent «Chelex-100" under complex economic conditions of native laboratories, which will optimize labour costs and improve the economic performance of DNA extraction protocol. Materials and methods. In order to conduct the research the samples of honey bees’ biological material: queen pupae exuviae, larvae of drone brood, some adult bees’ bodies (head and thorax) were selected. Bowl and drone brood were obtained from the experimental bee hives of Institute of Apiculture nd. a. P. I. Prokopovich of NAAS. DNA extraction from biosamples of Apis mellifera ssp. was carried out using «Chelex-100®» ion exchange resin in different concentrations and combinations. Before setting tests for determination of quantitative and quality indexes, dilution of DNA samples of the probed object was conducted in ratio 1:40. The degree of contamination with protein and polysaccharide fractions (OD 260/230), quantitative content of DNA (OD 260/280) in the extracted tests were conducted using spectrophotometer of «Biospec – nano» at the terms of sample volume in 2 µl and length of optical way in 0,7 mm [7]. Verification of DNA samples from biological material of bees, isolated by «Chelex-100®», was conducted after cold keeping during 24 hours at 20°C using PСR with primaries to the fragment of gene of quantitative trait locus (QTL) Sting-2 of next structure [8]:  3' – CTC GAC GAG ACG ACC AAC TTG – 5’; 3' – AAC CAG AGT ATC GCG AGT GTT AC – 5’ Program of amplification: 94 °C – 5 minutes – 1 cycle; 94 °C – 1 minute, 57°C – 1 minute, 72 °C – 2 minutes – 30 cycles; elongation after 72°C during 2 minutes – 1 cycle. The division of obtained amplicons was conducted by gel electrophoresis at a low current – 7 µÀ, in 1,5 % agarose gel (Sigma ®) in TAE buffer [7]. The results. At the time of optimization of DNA isolation methods, according to existing methods of foreign experts, it was found optimal volume of ion exchange resin solution was in the proposed concentration: instead of 60 µl of solution used 120 µl of «Chelex-100®», time of incubation was also amended from 30 minutes to 180 minutes [9]. The use of the author's combination of method «Chelex-100®» with lysis enzymes, proteinase K and detergents (1M dithiothreitol), as time of incubation was also amended, which was reduced to 180 minutes instead of the proposed 12 hours [10]. Changes in quality characteristics of obtained DNA in samples after reduction in incubation time were not found. Conclusions. The most economical method of DNA isolation from bees’ biological material is 20% solution of «Chelex-100» ion exchange resin with the duration of the incubation period of 180 minutes. It should also be noted that the best results can be obtained from exuviae, selected immediately after the queen’s exit from bowl, that reduces the likelihood of DNA molecules destruction under the influence of nucleases activation, but not later than 12 hours from release using the technology of isolated obtain of queens.

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