scholarly journals Withanolide A Biogeneration in in Vitro Shoot Cultures of Ashwagandha (Withania somnifera DUNAL), a Main Medicinal Plant in Ayurveda

2007 ◽  
Vol 55 (9) ◽  
pp. 1371-1375 ◽  
Author(s):  
Rajender Singh Sangwan ◽  
Narayan Das Chaurasiya ◽  
Payare Lal ◽  
Laxminarain Misra ◽  
Girish Chandra Uniyal ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
Shoot Cultures ◽  
Withanolide A ◽  
In Vitro Shoot Cultures

scholarly journals Glycowithanolides accumulation in in Vitro Shoot Cultures of Indian Ginseng (Withania somnifera Dunal)

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ashok Ahuja ◽  
Devinder Kaur ◽  
Mallubhotla Sharada ◽  
Arun Kumar ◽  
Krishan Avtar Suri ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Withania Somnifera ◽  
Renewable Resource ◽  
Multiple Shoot ◽  
Biologically Active ◽  
Shoot Cultures ◽  
Indian Ginseng ◽  
Multiple Shoot Cultures ◽  
In Vitro Shoot Cultures

Phytochemical investigations of multiple shoot cultures of selected accessions AGB002 and AGB025 of Withania somnifera. established in vitro utilizing shoot tip apices cultured on Murashige and Skoog's medium supplemented with BAP (1 mg/L) have been carried out. This has lead to isolation of four glycowithanolides viz. Withanoside IV (WSG-3), Withanoside VI (WSG-3A), Physagulin D (WSG-P) and Withastraronolide (WSC-O). The structures of these have been confirmed on the basis of spectroscopic data. Multiple shoot cultures could be an alternative renewable resource for production of these biologically active molecules.

scholarly journals Cryopreservation of aromatic ginger Kaempferia galanga L. by encapsulation-dehydration

2021 ◽  
Vol 13 (4) ◽  
pp. 11024
Author(s):  
Thankappan S. PREETHA ◽  
Achuthan S. HEMANTHAKUMAR ◽  
Peringatulli N. KRISHNAN
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Calcium Alginate ◽  
Apical Meristem ◽  
Shoot Tips ◽  
Rapid Freezing ◽  
Shoot Cultures ◽  
Kaempferia Galanga ◽  
In Vitro Shoot Cultures

Kaempferia galanga L. is an endangered multi-purpose medicinal plant in Family Zingiberaceae, the rhizomes of which are used for several ayurvedic formulations. Encapsulation-dehydration (ED) method was optimized for cryopreservation of shoot tips of K. galanga. Shoot tips (STs) bearing the apical meristem dissected from the established in vitro shoot cultures were preconditioned in MS+0.4 M sucrose prior to encapsulation in calcium alginate and the beads subsequently transferred to MS liquid+0.3 M sucrose for 3 days afterward dehydration inside the laminar airflow for 4 hours upon rapid freezing in LN and rapid thawing produced maximum 62.2% survival and 46.7% regeneration rates. Shoot regeneration was observed from the apical meristems exclusive of intermediary callus phase. The plantlets regenerated from cryopreserved STs transferred to the field were phenotypically analogous with the mother plant.

scholarly journals Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 670
Author(s):  
Katalin Magyar-Tábori ◽  
Nóra Mendler-Drienyovszki ◽  
Alexandra Hanász ◽  
László Zsombik ◽  
Judit Dobránszki
Keyword(s):  
Adverse Effects ◽  
Physiological Condition ◽  
Harmful Effect ◽  
Shoot Cultures ◽  
Virus Elimination ◽  
In Vitro Shoots ◽  
Further Development ◽  
In Vitro Shoot Cultures ◽  
Harmful Effects

In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice.

The Effectiveness of Selection for Salinity Tolerance Using In vitro Shoot Cultures

1988 ◽  
Vol 149 (2) ◽  
pp. 166-172 ◽  
Author(s):  
Stephen F. Chandler ◽  
Kee Yoeup Paek ◽  
Eng-Chong Pua ◽  
Elena Ragolsky ◽  
Binay B. Mandal ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
Shoot Cultures ◽  
Selection For ◽  
In Vitro Shoot Cultures

scholarly journals In Vitro Plant Growth Promoting Activity of Phyllocladane Diterpenoids Isolated from Callicarpa macrophylla Vahl. in Shoot Cultures of Rauwolfia serpentina

2007 ◽  
Vol 2 (8) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Manoj K Goel ◽  
Arun K Kukreja ◽  
Anil K Singh ◽  
Suman Preet S Khanuja
Keyword(s):  
Plant Growth ◽  
Shoot Cultures ◽  
Rauwolfia Serpentina ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Plant Tissue Cultures ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting ◽  
In Vitro Shoot Cultures

Phyllocladane diterpenoids, particularly calliterpenone (1) and calliterpenone monoacetate (2), isolated from leaves of Callicarpa macrophylla, produced significantly higher growth and multiplication of in vitro shoot cultures of Rauwolfia serpentina at 0.25 and 0.5 mg/L concentrations, respectively, compared to certain other plant growth regulators (0.1-5.0 mg/L) tested under in vitro conditions. This is the first report of the plant growth promoting activities of 1 and 2 in plant tissue cultures.

Biochemical study of root extract of Withania somnifera (L.)plant through HPLC analysis

2016 ◽  
Vol 36 (4) ◽  
Author(s):  
Naveen Gaurav ◽  
Arun Kumar ◽  
Aditi Grover ◽  
Deepak Som ◽  
U. K. Chauhan ◽  
...  
Keyword(s):  
High Performance ◽  
Withania Somnifera ◽  
Biochemical Study ◽  
Bioactive Metabolites ◽  
Root Extract ◽  
Ex Vitro ◽  
Withanolide A ◽  
Shoot Tip Explants ◽  
Quality Production

Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and withanolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).

Sign in / Sign up

Export Citation Format

Share Document