Transfer of Exogenous Macromolecules from Rat Stomach Wall to Blood and Lymph is Dependent on Molecular Weight.

1992 ◽  
Vol 40 (5) ◽  
pp. 1277-1279 ◽  
Author(s):  
Hiroshi YOSHIKAWA ◽  
Kanji TAKADA ◽  
Shozo MURANISHI
Keyword(s):  
Molecular Weight ◽  
Stomach Wall ◽  
Rat Stomach

Hemorrhagic Shock Activates Mast Cells in the Rat Stomach Wall

2000 ◽  
Vol 10 (03) ◽  
pp. 155-161 ◽  
Author(s):  
W. Debek ◽  
M. Barczyk ◽  
L. Chyczewski ◽  
W. Roszkowska-Jakimiec
Keyword(s):  
Mast Cells ◽  
Hemorrhagic Shock ◽  
Stomach Wall ◽  
Rat Stomach

scholarly journals Arteriovenous differences for amino acids across control and acid-secreting rat stomach in vivo

1983 ◽  
Vol 210 (2) ◽  
pp. 451-455 ◽  
Author(s):  
N G Anderson ◽  
P J Hanson
Keyword(s):  
Amino Acids ◽  
Ammonia Concentration ◽  
Branched Chain Amino Acids ◽  
Stomach Wall ◽  
Rat Stomach ◽  
Branched Chain Amino Acid ◽  
Acid Secreting ◽  
Branched Chain ◽  
Stimulation Of

1. A method is described for measuring arteriovenous differences across the rat stomach in vivo. 2. Notable results were the uptake of branched-chain amino acids, the uptake of arginine, which was approximately balanced by an output of ornithine, and the output of alanine. 3. The fractional extraction of glutamine from the blood by the stomach wall of pentagastrin-stimulated rats was 4.7%. 4. The arteriovenous differences for ammonia depended upon the blood ammonia concentration. 5. Arteriovenous differences were not affected by the stimulation of acid secretion with pentagastrin. 6. It is concluded that the high activity of branched-chain-amino-acid aminotransferase (EC 2.6.1.42) in the gastric mucosa is associated with metabolism of these amino acids, but that the stomach wall is a less avid user of glutamine than is the small intestine.

Angiotensin metabolism in rat stomach wall: prevalence of angiotensin-(1-7) formation

2009 ◽  
Author(s):  
Maciej Suski ◽  
Rafał Olszanecki ◽  
Józef Madej ◽  
Anna Gebska ◽  
Beata Bujak-Giżycka ◽  
...  
Keyword(s):  
Stomach Wall ◽  
Rat Stomach

QUANTITATIVE EFFECT OF SOME STEROID HORMONES ON THE MUCOSAL MAST CELLS AND TISSUE EOSINOPHILS IN THE RAT STOMACH WALL

1961 ◽  
Vol 37 (1) ◽  
pp. 153-160 ◽  
Author(s):  
Toimi Räsänen
Keyword(s):  
Mast Cells ◽  
Gastric Mucosa ◽  
Steroid Hormones ◽  
Lamina Propria ◽  
Stomach Wall ◽  
Rat Stomach ◽  
Intestinal Canal ◽  
Tissue Eosinophilia ◽  
Mucosal Mast Cells ◽  
Higher Doses

ABSTRACT Prednisolone (11β,17,21-trihydroxy-pregna-1,4-diene-3,20-dione) injected into rats at 6 hour intervals for 48 hours causes degranulation of the mast cells of the gastric mucosa to an extent dependent on the dose. Tissue eosinophilia in the gastric lamina propria decreased more slowly with the smallest dose, but clearly with the higher doses. Deoxycorticosterone (21-hydroxy-pregn-4-ene-3,20-dione) in doses of varying size produced no definite degranulation of the mast cells of the gastric mucosa, nor any changes in the tissue eosinophilia of the lamina propria. It is suggested that glucocorticoids conjugate in the lamina propria of the gastro-intestinal canal with the polysaccharides of the mast granules, and that the tissue eosinophils eliminate the histamine and serotonin liberated.

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

The External Shape of Human γ GI Immunoglobulin from Crystal Sections

1971 ◽  
Vol 29 ◽  
pp. 428-429
Author(s):  
L. W. Labaw
Keyword(s):  
Molecular Weight ◽  
Sodium Phosphate ◽  
Osmium Tetroxide ◽  
Unit Cell ◽  
Monoclinic Space Group ◽  
X Ray ◽  
Large Molecular Weight ◽  
Cell Dimensions ◽  
Unit Cell Dimensions ◽  
Crystallographic Axes

Crystals of a human γGl immunoglobulin have the external morphology of diamond shaped prisms. X-ray studies have shown them to be monoclinic, space group C2, with 2 molecules per unit cell. The unit cell dimensions are a = 194.1, b = 91.7, c = 51.6Å, 8 = 102°. The relatively large molecular weight of 151,000 and these unit cell dimensions made this a promising crystal to study in the EM.Crystals similar to those used in the x-ray studies were fixed at 5°C for three weeks in a solution of mother liquor containing 5 x 10-5M sodium phosphate, pH 7.0, and 0.03% glutaraldehyde. They were postfixed with 1% osmium tetroxide for 15 min. and embedded in Maraglas the usual way. Sections were cut perpendicular to the three crystallographic axes. Such a section cut with its plane perpendicular to the z direction is shown in Fig. 1.This projection of the crystal in the z direction shows periodicities in at least four different directions but these are only seen clearly by sighting obliquely along the micrograph.

Conformation of eukaryotic ribosomal RNAs

1983 ◽  
Vol 41 ◽  
pp. 592-593
Author(s):  
M. Boublik ◽  
G. Thornton ◽  
G. Oostergetel ◽  
J.F. Hainfeld ◽  
J.S. Wall
Keyword(s):  
Molecular Weight ◽  
Mass Measurement ◽  
Carbon Film ◽  
Structural Complexity ◽  
Scanning Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Pure Nitrogen ◽  
Freeze Dried ◽  
Scanning Transmission ◽  
Partially Unfolded

Understanding the structural complexity of ribosomes and their role in protein synthesis requires knowledge of the conformation of their components - rRNAs and proteins. Application of dedicated scanning transmission electron microscope (STEM), electrical discharge of the support carbon film in an atmosphere of pure nitrogen, and determination of the molecular weight of individual rRNAs enabled us to obtain high resolution electron microscopic images of unstained freeze-dried rRNA molecules from BHK cells in a form suitable for evaluation of their 3-D structure. Preliminary values for the molecular weight of 28S RNA from the large and 18S RNA from the small ribosomal subunits as obtained by mass measurement were 1.84 x 106 and 0.97 x 106, respectively. Conformation of rRNAs consists, in general, of alternating segments of intramolecular hairpin stems and single stranded loops in a proportion which depends on their ionic environment, the Mg++ concentration in particular. Molecules of 28S RNA (Fig. 1) and 18S RNA (not shown) obtained by freeze-drying from a solution of 60 mM NH+4 acetate and 2 mM Mg++ acetate, pH 7, appear as partially unfolded coils with compact cores suggesting a high degree of ordered secondary structure.

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