Angiotensin metabolism in rat stomach wall: prevalence of angiotensin-(1-7) formation

2009 ◽  
Author(s):  
Maciej Suski ◽  
Rafał Olszanecki ◽  
Józef Madej ◽  
Anna Gebska ◽  
Beata Bujak-Giżycka ◽  
...  
Keyword(s):  
Stomach Wall ◽  
Rat Stomach

Hemorrhagic Shock Activates Mast Cells in the Rat Stomach Wall

2000 ◽  
Vol 10 (03) ◽  
pp. 155-161 ◽  
Author(s):  
W. Debek ◽  
M. Barczyk ◽  
L. Chyczewski ◽  
W. Roszkowska-Jakimiec
Keyword(s):  
Mast Cells ◽  
Hemorrhagic Shock ◽  
Stomach Wall ◽  
Rat Stomach

scholarly journals Arteriovenous differences for amino acids across control and acid-secreting rat stomach in vivo

1983 ◽  
Vol 210 (2) ◽  
pp. 451-455 ◽  
Author(s):  
N G Anderson ◽  
P J Hanson
Keyword(s):  
Amino Acids ◽  
Ammonia Concentration ◽  
Branched Chain Amino Acids ◽  
Stomach Wall ◽  
Rat Stomach ◽  
Branched Chain Amino Acid ◽  
Acid Secreting ◽  
Branched Chain ◽  
Stimulation Of

1. A method is described for measuring arteriovenous differences across the rat stomach in vivo. 2. Notable results were the uptake of branched-chain amino acids, the uptake of arginine, which was approximately balanced by an output of ornithine, and the output of alanine. 3. The fractional extraction of glutamine from the blood by the stomach wall of pentagastrin-stimulated rats was 4.7%. 4. The arteriovenous differences for ammonia depended upon the blood ammonia concentration. 5. Arteriovenous differences were not affected by the stimulation of acid secretion with pentagastrin. 6. It is concluded that the high activity of branched-chain-amino-acid aminotransferase (EC 2.6.1.42) in the gastric mucosa is associated with metabolism of these amino acids, but that the stomach wall is a less avid user of glutamine than is the small intestine.

QUANTITATIVE EFFECT OF SOME STEROID HORMONES ON THE MUCOSAL MAST CELLS AND TISSUE EOSINOPHILS IN THE RAT STOMACH WALL

1961 ◽  
Vol 37 (1) ◽  
pp. 153-160 ◽  
Author(s):  
Toimi Räsänen
Keyword(s):  
Mast Cells ◽  
Gastric Mucosa ◽  
Steroid Hormones ◽  
Lamina Propria ◽  
Stomach Wall ◽  
Rat Stomach ◽  
Intestinal Canal ◽  
Tissue Eosinophilia ◽  
Mucosal Mast Cells ◽  
Higher Doses

ABSTRACT Prednisolone (11β,17,21-trihydroxy-pregna-1,4-diene-3,20-dione) injected into rats at 6 hour intervals for 48 hours causes degranulation of the mast cells of the gastric mucosa to an extent dependent on the dose. Tissue eosinophilia in the gastric lamina propria decreased more slowly with the smallest dose, but clearly with the higher doses. Deoxycorticosterone (21-hydroxy-pregn-4-ene-3,20-dione) in doses of varying size produced no definite degranulation of the mast cells of the gastric mucosa, nor any changes in the tissue eosinophilia of the lamina propria. It is suggested that glucocorticoids conjugate in the lamina propria of the gastro-intestinal canal with the polysaccharides of the mast granules, and that the tissue eosinophils eliminate the histamine and serotonin liberated.

Transfer of Exogenous Macromolecules from Rat Stomach Wall to Blood and Lymph is Dependent on Molecular Weight.

1992 ◽  
Vol 40 (5) ◽  
pp. 1277-1279 ◽  
Author(s):  
Hiroshi YOSHIKAWA ◽  
Kanji TAKADA ◽  
Shozo MURANISHI
Keyword(s):  
Molecular Weight ◽  
Stomach Wall ◽  
Rat Stomach

Electron-Microscopic localization of biotinylated gastrin bound to eosinophils in the rat stomach

1994 ◽  
Vol 52 ◽  
pp. 300-301
Author(s):  
Caroline A. Miller ◽  
David H. Nichols ◽  
Richard F. Murphy
Keyword(s):  
Electron Microscope ◽  
Phosphate Buffer ◽  
Uranyl Acetate ◽  
List Type ◽  
Cerium Chloride ◽  
Rat Stomach ◽  
Electron Microscopic ◽  
Human Sequence ◽  
Acetate Lead ◽  
Microscope Slides

Gastrin is a small peptide capable of both stimulating gastric acid secretion and acting as an enteric growth factor. Known functions of eosinophils in the rat stomach are related to immunological defense. Here we demonstrate the binding of biotinylated gastrin to rat stomach eosinophils in the electron microscope. Small pieces of stomach were fixed by immersion in 4% paraformaldehyde/0.1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4 for 1 hour. The tissue was then cryoprotected in 30% sucrose/0.1 M phosphate buffer, transferred to Tissue Tek OCT compound and frozen in isopentane cooled with liquid nitrogen. Transverse cryostat sections were cut at 25 μm, thawed in PBS and free floating sections exposed to 10−5 M biotinylated 1-17 gastrin (human sequence; Peninsula Labs) for 1 hour. Controls omitted the biotinylated gastrin from this step. Sections were then rinsed 3X in PBS and exposed to either:1).a 1:50 dilution of 10 nm Extravidin colloidal gold (Sigma) for 2 hours, or2).an avidin-biotin-alkaline phosphatase complex (ABC-AP;Vector) for 1 hour. A substrate solution containing cerium chloride was used to generate an electron dense reaction product.Sections from both procedures were postfixed in 1% OsO4 in 0.1 M phosphate buffer, rinsed and dehydrated. These were then flat embedded in EMbed 812 between two microscope slides coated with Liquid Release (both from Electron Microscopy Sciences).Polymerized sections were adhered to resin blocks using super glue, cut at 70-90 nm, stained with uranyl acetate/lead citrate and observed in a Philips CM-10 electron microscope.

Effect of Interleukin-8 on histamine release from isolated rat stomach

2001 ◽  
Vol 120 (5) ◽  
pp. A159-A159
Author(s):  
S RO ◽  
K YAKABI ◽  
T NAKAMURA
Keyword(s):  
Histamine Release ◽  
Interleukin 8 ◽  
Rat Stomach ◽  
Isolated Rat

Effect of galanin on gastrin release from the isolated rat stomach

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S31
Author(s):  
TH. SEUFFERLEIN ◽  
V. SCHUSDZIARRA ◽  
S. MADAUS ◽  
M. CLASSEN
Keyword(s):  
Gastrin Release ◽  
Rat Stomach ◽  
Isolated Rat
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