scholarly journals Inhibition of succinoxidase and reduced nicotinamide adenine dinucleotide (NADH) oxidase by 2,3-ethlenedioxy-1,4-benzoquinones having alkylthio and arythio side chains.

1989 ◽  
Vol 37 (1) ◽  
pp. 221-225 ◽  
Author(s):  
Koichi MORI ◽  
Chieko TERASHITA ◽  
Yuko FUKUNAGA ◽  
Tadashi OKAMOTO ◽  
Taeko KISHI ◽  
...  
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Nadh Oxidase ◽  
Nicotinamide Adenine ◽  
Side Chains ◽  
Reduced Nicotinamide Adenine Dinucleotide

LOCATION OF PROTEINASE, PEPTIDASE, CATALASE, AND NADH OXIDASE IN CELLS OF STAPHYLOCOCCUS LACTIS

1964 ◽  
Vol 10 (2) ◽  
pp. 197-200 ◽  
Author(s):  
I. J. McDonald
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Membrane Fraction ◽  
Nadh Oxidase ◽  
Nicotinamide Adenine ◽  
Differential Centrifugation ◽  
Reduced Nicotinamide Adenine Dinucleotide ◽  
In Cells

Cells of a strain of Staphylococcus lactis were disrupted by lysozyme treatment and fractionated by differential centrifugation. Proteinase and reduced nicotinamide adenine dinucleotide oxidase were found in the membrane fraction; peptidase and catalase were found in the cytoplasm. Similar results were obtained when cells were broken by sonic oscillation. Proteinase of this organism is considered to be a surface-bound enzyme.

scholarly journals Cytochemical localization of NADH oxidase in Candida albicans.

1977 ◽  
Vol 25 (3) ◽  
pp. 193-199 ◽  
Author(s):  
M Borgers ◽  
S De Nollin ◽  
F Thoné ◽  
H Van Belle
Keyword(s):  
Candida Albicans ◽  
Nicotinamide Adenine Dinucleotide ◽  
Oxidase Activity ◽  
Nadh Oxidase ◽  
Lethal Effect ◽  
Nicotinamide Adenine ◽  
Strong Increase ◽  
Cytochemical Localization ◽  
Prolonged Incubation ◽  
Reduced Nicotinamide Adenine Dinucleotide

The application of a recently published technique to localize reduced nicotinamide adenine dinucleotide oxidase activity is described in glutaraldehyde-fixed Candida albicans. The reaction product appears as a finely granular precipitate on the mitochondrial cristae and on the central vacuolar membrane, and, if present, on the vacuolar contents. Fixation should be kept to a minimum and prolonged incubation times up to 2 hr are necessary to show these reactive sites. The reaction appears to be strongly substrate-dependent and not affected by cyanide. Exposure of C. albicans cells to the antimycotic miconazole resulted in a strong increase in reduced nicotinamide, adenine dinucleotide and oxidase activity. The hypothesis is put forward that this enzyme, together with peroxidative and catalatic enzymes, may be implicated in the mechanism by which miconazole exerts its lethal effect on C. albicans.

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