scholarly journals Synthesis of a 37-residue peptide amide corresponding to the entire amino acid sequence of .ALPHA.-form of rat calcitonin gene-related peptide (.ALPHA.-rCGRP).

1987 ◽  
Vol 35 (1) ◽  
pp. 60-71 ◽  
Author(s):  
KENSHI ANDO ◽  
KOUKI KITAGAWA ◽  
SHINYA KIYAMA ◽  
TATSUHIKO KAWAMOTO ◽  
TADASHI AKITA ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Peptide Amide

Isolation and amino acid sequence of calcitonin gene related peptide from porcine spinal cord

Neuropeptides ◽  
1987 ◽  
Vol 9 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Sadao Kimura ◽  
Yoshiki Sugita ◽  
Ichiro Kanazawa ◽  
Akira Saito ◽  
Katsutoshi Golo
Keyword(s):  
Spinal Cord ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

scholarly journals Selective Inactivation of Adrenomedullin over Calcitonin Gene-related Peptide Receptor Function by the Deletion of Amino Acids 14-20 of the Mouse Calcitonin-like Receptor

2004 ◽  
Vol 279 (19) ◽  
pp. 20387-20391 ◽  
Author(s):  
Daniela Koller ◽  
Lars M. Ittner ◽  
Roman Muff ◽  
Knut Husmann ◽  
Jan A. Fischer ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Peptide Hormone ◽  
Receptor Function ◽  
Cgrp Receptor ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
N Terminus ◽  
Camp Formation

The receptors for the neuropeptide calcitonin (CT) gene-related peptide (CGRP) and the multifunctional peptide hormone adrenomedullin (AM) are calcitonin-like receptor (CLR)/receptor-activity-modifying protein (RAMP) 1 and CLR/RAMP2 heterodimers, respectively. Here, the amino acid sequence TRNKIMT, corresponding to the residues 14-20 of the N terminus of the mouse (m) CLR, was found to be required for a functional mCLR/RAMP2 AM receptor. The deletion of amino acids 14-20 (Δ14-20) or their substitution by alanine (14-20A) did not affect the heterodimerization of the mCLR with mRAMP1 or mRAMP2, and the levels of expression at the surface of transiently transfected COS-7 cells were not altered. In mRAMP1/mCLR- or mRAMP1/mCLR-(Δ14-20)-expressing cells CGRP stimulated cAMP formation with EC50values of 0.12 ± 0.01 and 1.5 ± 0.4 nm, respectively. In mRAMP2/mCLR-expressing cells the EC50of AM was 0.8 ± 0.2 nm. However, in cells expressing mRAMP2/mCLR-(Δ14-20) up to 10-6mAM failed to stimulate cAMP production. In mRAMP2/mCLR-(14-20A) expressing cells the cAMP response to AM was minimally restored, and the EC50was >100 nm. In conclusion, the deletion of the amino acid sequence TRNKIMT of the extreme N terminus of the mCLR maintained CGRP receptor function of mRAMP1/receptor heterodimers, but AM no longer activated the mutant mCLR-(Δ14-20) in the presence of mRAMP2. The TRNKIMT sequence is required for normal mCLR/mRAMP2 association, and as a consequence, high affinity AM binding signaling the activation of adenylyl cyclase.

Cellular distribution of PACAP-38 and PACAP receptors in the rat brain: Relation to migraine activated regions

Cephalalgia ◽  
2019 ◽  
Vol 40 (6) ◽  
pp. 527-542 ◽  
Author(s):  
Karin Warfvinge ◽  
Lars Edvinsson
Keyword(s):  
Amino Acid ◽  
Rat Brain ◽  
Vasoactive Intestinal Polypeptide ◽  
Calcitonin Gene Related Peptide ◽  
Amino Acid Sequences ◽  
Primary Headaches ◽  
Functional Responses ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Intestinal Polypeptide

Background Pituitary adenylate cyclase-activating polypeptide (PACAP) occurs as either a 27- or 38-amino acid neuropeptide and belongs to the vasoactive intestinal polypeptide/glucagon/secretin family of peptides. PACAP and vasoactive intestinal polypeptide have a 68% homology of their amino acid sequences and share three B-type G-protein coupled receptors: VPAC1, VPAC2 and PAC1 receptors. Methods/results The distribution of PACAP-38 and its receptors in the brain is only partly described in the literature. Here, we have performed a study to provide the more general picture of this system in rat brain in order to understand a putative role in primary headaches and partly in relation to the calcitonin gene-related peptide system. We observed a rich expression of PACAP-38 and PAC1 receptor immunoreactivity in many regions throughout the cerebrum, cerebellum and brainstem. The expression pattern points to multiple functions, not least associated with pain and reactions to pain. The expression of VPAC1 and VPAC2 receptor immunoreactivity was very sparse. In several regions such as the cerebral cortex, trigeminal nucleus caudalis, hypothalamus and pons there was a close relation to calcitonin gene-related peptide expression. Conclusion The findings suggest that the rich supply of PACAP-38 and PAC1 receptors is associated with basic functional responses in the central nervous system (CNS), and there are important close anatomical relations with calcitonin gene-related peptide in CNS regions associated with migraine pathophysiology.

Receptors for calcitonin, calcitonin gene related peptide, amylin, and adrenomedullin

1995 ◽  
Vol 73 (7) ◽  
pp. 963-967 ◽  
Author(s):  
Roman Muff ◽  
Walter Born ◽  
Jan A. Fischer
Keyword(s):  
Amino Acid ◽  
Disulfide Bridge ◽  
Calcitonin Gene Related Peptide ◽  
Ring Structure ◽  
Cross Reactivity ◽  
Related Peptide ◽  
C Terminus ◽  
Calcitonin Gene ◽  
G Protein Coupled ◽  
Biological Actions

Calcitonin, calcitonin gene related peptide, amylin, and adrenomedullin are structurally related polypeptides characterized by a six or seven amino acid ring structure linked by a disulfide bridge and an amidated C-terminus. They exhibit overlapping biological actions as a result of cross-reactivity between the different receptors. In this article, the respective receptors and G-protein-coupled postreceptor events are reviewed in relation to some of the biological actions of the peptides.Key words: adrenomedullin, amylin, calcitonin gene related peptide, cyclic AMP, receptors.

Calcitonin gene-related peptide regulates amino acid absorption across rat jejunum

2000 ◽  
Vol 90 (1-3) ◽  
pp. 39-45 ◽  
Author(s):  
Kassem A Barada ◽  
Nayef.E Saadé ◽  
Samir F Atweh ◽  
Carmen I Khoury ◽  
Camille F Nassar
Keyword(s):  
Amino Acid ◽  
Calcitonin Gene Related Peptide ◽  
Rat Jejunum ◽  
Acid Absorption ◽  
Related Peptide ◽  
Amino Acid Absorption ◽  
Calcitonin Gene
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