Studies on peptides. CXXXVI. Solution-phase synthesis of a 37-residue peptide amide corresponding to the entire amino acid sequence of human calcitonin gene-related peptide (hCGRP).

1986 ◽  
Vol 34 (2) ◽  
pp. 613-620 ◽  
Author(s):  
NOBUTAKA FUJII ◽  
AKIRA OTAKA ◽  
SUSUMU FUNAKOSHI ◽  
MOTOYOSHI NOMIZU ◽  
KENICHI AKAJI ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Calcitonin Gene Related Peptide ◽  
Solution Phase ◽  
Human Calcitonin ◽  
Phase Synthesis ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Solution Phase Synthesis ◽  
Peptide Amide

Isolation and amino acid sequence of calcitonin gene related peptide from porcine spinal cord

Neuropeptides ◽  
1987 ◽  
Vol 9 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Sadao Kimura ◽  
Yoshiki Sugita ◽  
Ichiro Kanazawa ◽  
Akira Saito ◽  
Katsutoshi Golo
Keyword(s):  
Spinal Cord ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

scholarly journals Calcitonin gene-related peptide receptor antagonist human CGRP-(8-37)

1989 ◽  
Vol 256 (2) ◽  
pp. E331-E335 ◽  
Author(s):  
T. Chiba ◽  
A. Yamaguchi ◽  
T. Yamatani ◽  
A. Nakamura ◽  
T. Morishita ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Adenylate Cyclase ◽  
Calcitonin Gene Related Peptide ◽  
Human Calcitonin ◽  
Liver Plasma Membrane ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Liver Membranes ◽  
Calcitonin Receptors ◽  
Stimulation Of

From this study, we predicted that the human calcitonin gene-related peptide (hCGRP) fragment hCGRP-(8-37) would be a selective antagonist for CGRP receptors but an agonist for calcitonin (CT) receptors. In rat liver plasma membrane, where CGRP receptors predominate and CT appears to act through these receptors, hCGRP-(8-37) dose dependently displaced 125I-[Tyr0]rat CGRP binding. However, hCGRP-(8-37) had no effect on adenylate cyclase activity in liver plasma membrane. Furthermore, hCGRP-(8-37) inhibited adenylate cyclase activation induced not only by hCGRP but also by hCT. On the other hand, in LLC-PK1 cells, where calcitonin receptors are abundant and CGRP appears to act via these receptors, the bindings of 125I-[Tyr0]rat CGRP and 125I-hCT were both inhibited by hCGRP-(8-37). In contrast to liver membranes, interaction of hCGRP-(8-37) with these receptors led to stimulation of adenosine 3',5'-cyclic monophosphate (cAMP) production in LLC-PK1 cells, and moreover, this fragment did not inhibit the increased production of cAMP induced not only by hCT but also by hCGRP. Thus hCGRP-(8-37) appears to be a useful tool for determining whether the action of CGRP as well as that of CT is mediated via specific CGRP receptors or CT receptors.

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