scholarly journals Analytical Studies on Esterases using Gas-Liquid Chromatography. II. On the Alcoholytic and Hydrolytic Activities of Esterases from Swine Liver and Mucor javanicus

1974 ◽  
Vol 22 (2) ◽  
pp. 413-420 ◽  
Author(s):  
HIDEKO ISHIHARA ◽  
HIROH IKEZAWA ◽  
SETSUZO TEJIMA
Keyword(s):  
Liquid Chromatography ◽  
Gas Liquid Chromatography ◽  
Analytical Studies ◽  
Hydrolytic Activities ◽  
Swine Liver ◽  
Mucor Javanicus

Confirmatory Identification of Carbadox-Related Residues in Swine Liver by Gas-Liquid Chromatography/Mass Spectrometry with Selected Ion Monitoring

1982 ◽  
Vol 65 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Martin J Lynch ◽  
S Richard Bartolucci
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Carboxylic Acid ◽  
Gas Liquid Chromatography ◽  
Target Tissue ◽  
Liquid Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Methyl Ester Derivative ◽  
Chromatography Mass Spectrometry ◽  
Swine Liver

Abstract A confirmatory method has been developed to identify quinoxaline-2-carboxylic acid, the carbadox tissue residue, in swine liver, a target tissue, at the regulatory level of 30 μg/kg. Quinoxaline-2-carboxylic acid (QCA) is isolated from liver hydrolysates by solvent extraction and ion-exclusion chromatography, and a methyl ester derivative (CP-25,536 or QME) is identified by gas-liquid chromatography/ mass spectrometry with selected ion monitoring. The relative intensities of 3 ions: the base peak at m/z = 130, a second significant mass at m/z = 158, and the molecular ion (M+) at m/z = 188, are monitored simultaneously with a quadrupole mass spectrometer. Validation studies consisting of the analysis of liver fortified with QCA at the regulatory level and analysis of swine specimens containing physiologically incurred carbadox residues demonstrated that peak height ratios of ions in these tissue extracts corresponded to ion intensities of standards monitored at m/z = 188,158, and 130.

A study of asphalt-aggregate interactions using inverse gas-liquid chromatography

1974 ◽  
Vol 24 (11) ◽  
pp. 645-654 ◽  
Author(s):  
F. Alan Barbour ◽  
Richard V. Barbour ◽  
J. Claine Peterson
Keyword(s):  
Liquid Chromatography ◽  
Gas Liquid Chromatography

GASCHROMATOGRAPHIC DETERMINATION OF STEROIDS IN THE URINE OF PATIENTS WITH CUSHING'S SYNDROME

1971 ◽  
Vol 67 (2) ◽  
pp. 303-315 ◽  
Author(s):  
A. J. Moolenaar ◽  
A. P. van Seters
Keyword(s):  
Liquid Chromatography ◽  
Cushing’S Syndrome ◽  
Normal Subjects ◽  
Diagnostic Value ◽  
Cushing's Syndrome ◽  
Gas Liquid Chromatography ◽  
Obese Subjects

ABSTRACT The 17-oxosteroids were estimated in the urine of 27 patients with Cushing's syndrome by gas-liquid chromatography (G. L. C.). The values of the various steroid fractions are compared with those of normal subjects, patients with thyrotoxicosis and obese subjects. The effect of the age of the patients on the diagnostic value of the invidual 17-oxosteroids and their ratios is discussed.

Simultaneous Determination of Lactulose and Mannitol in Urine of Burn Patients by Gas-Liquid Chromatography

1992 ◽  
Author(s):  
Ronald L. Shippee ◽  
Avery A. Johnson ◽  
William G. Cioffi ◽  
James Lasko ◽  
Thomas E. LeVoyer
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Gas Liquid Chromatography ◽  
Burn Patients

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

Concentrations of non-glucose polyols in serum and cerebrospinal fluid from apparently healthy adults and children.

1984 ◽  
Vol 30 (2) ◽  
pp. 188-191 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
S Seki ◽  
K Seki
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
Metabolic Diseases ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Adults And Children ◽  
Healthy Persons ◽  
Apparently Healthy

Abstract Six non-glucose polyols--mannose, fructose, 1-deoxyglucose, mannitol, glucitol, and inositol--were identified and evaluated in human serum and cerebrospinal fluid by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Concentrations of fructose, mannose, and inositol in the serum of healthy persons or children without metabolic diseases varied with age, as already reported for 1-deoxyglucose. Fructose, inositol, and glucitol concentrations in cerebrospinal fluid significantly exceeded those in serum. The method described here for determining polyols and for evaluating polyol patterns in serum, as well as the resulting data on children and healthy subjects, should be useful in investigations of the clinical and physiological significance of polyols.

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