Simultaneous Determination of Lactulose and Mannitol in Urine of Burn Patients by Gas-Liquid Chromatography

1992 ◽  
Author(s):  
Ronald L. Shippee ◽  
Avery A. Johnson ◽  
William G. Cioffi ◽  
James Lasko ◽  
Thomas E. LeVoyer
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Gas Liquid Chromatography ◽  
Burn Patients

Simultaneous Determination of Lactulose and Mannitol in Urine of Burn Patients by Gas-Liquid Chromatography

1992 ◽  
Vol 38 (3) ◽  
pp. 343-345 ◽  
Author(s):  
R L Shippee ◽  
A A Johnson ◽  
W G Cioffi ◽  
J Lasko ◽  
T E LeVoyer ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Urine Samples ◽  
Gas Liquid Chromatography ◽  
Burn Patients ◽  
Mucosal Permeability ◽  
Severe Burns ◽  
Urine Concentrations ◽  
Enzymatic Methods

Abstract Ratios of lactulose/mannitol excretion in urine have been used to assess the extent of intestinal permeability in various disease and trauma conditions. Reported studies have used this technique to correlate altered gastrointestinal mucosal permeability to translocation of bacteria and endotoxin, leading to occult sepsis in burn patients. Enzymatic methods of analysis for urine concentrations of mannitol and lactulose were used in these studies. We have found that urine from patients with severe burns frequently contains compounds that interfere with the enzymatic methods. We describe using gas-liquid chromatography to determine mannitol and lactulose simultaneously in the urine of burn patients. To avoid the multiple peaks for the anomeric forms of the reducing sugars during precolumn trimethylsilyl derivatization, we converted the sugars to oximes before the silylation step. The method gave good recoveries of mannitol and lactulose added to burn patients' urine samples. Unlike the enzymatic methods, gas-liquid chromatography eliminates the effect of interfering compounds and allows for the simultaneous determination of both sugars in urine samples.

Simultaneous Determination of Trimethadione and Its Metabolite, Dimethadione, by Gas—Liquid Chromatography

1971 ◽  
Vol 17 (7) ◽  
pp. 607-609 ◽  
Author(s):  
H E Booker ◽  
B Darcey
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Major Metabolite ◽  
Gas Liquid Chromatography ◽  
Single Extraction ◽  
Chromatographic Procedure

Abstract A simple gas chromatographic procedure is described for the measurement of trimethadione and its major metabolite, dimethadione, in serum. The method, with which compounds can be determined on a single extraction and injection, is reliable and free from interference caused by other drugs, particularly those that the patient is likely to be taking in combination with trimethadione.

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