scholarly journals Studies on Metabolism of Drugs. XIII. Quantitative Separation of Metabolites in Human Urine after Oral Administration of Sulfisomezole and Sulfaphenazole

1972 ◽  
Vol 20 (9) ◽  
pp. 2047-2050 ◽  
Author(s):  
MICHIHIRO UEDA ◽  
KIYOSHI ORITA ◽  
TAMOTSU KOIZUMI
Keyword(s):  
Oral Administration ◽  
Human Urine ◽  
Quantitative Separation

Effect of Pretreatment of Rats with an Urinary Preparation on Liver Injuries Induced by Carbon Tetrachloride and α-Naphthylisothiocyanate

1998 ◽  
Vol 26 (03n04) ◽  
pp. 343-351 ◽  
Author(s):  
Tung-Yuan Lai ◽  
Yueh-Wern Wu ◽  
Jaung-Geng Lin ◽  
Wen-Chuan Lin
Keyword(s):  
Lipid Peroxidation ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Oral Administration ◽  
Human Urine ◽  
Acute Liver Injury ◽  
Indirect Pathway ◽  
Liver Injuries ◽  
Hepatic Glutathione ◽  
Biochemical Observation

The effect of oral administration of a preparation of human urine (PHU) on the progression of acute liver injury was examined in rats intoxicated with carbon tetrachloride (CCl4) and α-naphthylisothiocyanate (ANIT). PHU protected the liver from CCl4-induced injury as judged by morphological and biochemical observations. In contrast, PHU aggravated ANIT-induced injury as judged also by morphological and biochemical observation. PHU prevented the increase in hepatic glutathione (GSH) and lipid peroxidation induced by CCl4. But PHU enhanced the increase in hepatic GSH caused by ANIT. These results indicate that the effect of PHU on hepatic GSH concentrations is through an indirect pathway. Clinical application of PHU on hepatitis should be explored further.

scholarly journals Studies on Metabolism of Drugs. IX. On the Excreted Substances in the Human Urine after Oral Administration of Sulfamethomidine

1968 ◽  
Vol 16 (2) ◽  
pp. 352-356 ◽  
Author(s):  
MICHIHIRO UEDA ◽  
NORIHIKO MURAKAMI ◽  
KAZUKO FURUKI ◽  
HARUMI ATSUMURA
Keyword(s):  
Oral Administration ◽  
Human Urine

scholarly journals Fast and Sensitive Screening of Oxandrolone and Its Major Metabolite 17-Epi-Oxandrolone in Human Urine by UHPLC—MS/MS with On-Line SPE Sample Pretreatment

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 480
Author(s):  
Jaroslav Galba ◽  
Juraj Piešťanský ◽  
Andrej Kováč ◽  
Dominika Olešová ◽  
Ondrej Cehlár ◽  
...  
Keyword(s):  
Oral Administration ◽  
Mass Spectrometer ◽  
Human Urine ◽  
Solid Phase ◽  
Major Metabolite ◽  
Renal Elimination ◽  
Time Interval ◽  
Highly Sensitive ◽  
On Line

Oxandrolone, a synthetic testosterone analog, is used for the treatment of several diseases associated with weight loss. Unfortunately, oxandrolone is abused by many athletes and bodybuilders due to its strong anabolic effect. We have developed and validated a highly sensitive and rapid on-line SPE-UHPLC-MS/MS method for the determination of oxandrolone and simultaneous identification of its major metabolite 17-epi-oxandrolone in urine matrices. Enrichment of the analytes via an integrated solid-phase extraction was achieved using an Acquity UPLC BEH C18 Column. Subsequently, the chromatographic separation of the on-line preconcentrated sample fraction was achieved using an Acquity HSS T3 C18 Column. For the structural identification of these analytes, a high-resolution mass spectrometer Synapt-G2Si coupled to the Acquity M-class nano-LC system with ionKey source was used. A highly sensitive determination of oxandrolone was achieved using a tandem quadrupole mass spectrometer XEVO TQD. The method was successfully validated in the linear range of oxandrolone from 81.63 pg·mL−1 (limit of quantification, LOQ) to 5000 pg·mL−1 in the human urine matrix. It was applied to the analysis of real urine samples obtained from a healthy volunteer after the oral administration of one dose (10 mg) of oxandrolone. Concentration vs. time dependence was tested in the time interval of 4 h–12 days (after oral administration) to demonstrate the ability of the method to detect the renal elimination of oxandrolone from the human body. Favorable performance parameters along with successful application indicate the usefulness of the proposed method for its routine use in antidoping control labs.

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