scholarly journals Studies on Bile Acids and Bile Alcohols. I. Gas Liquid Chromatography of Human Bile Acids.

1964 ◽  
Vol 12 (6) ◽  
pp. 710-713 ◽  
Author(s):  
Kyosuke Tsuda ◽  
Yoshihiro Sato ◽  
Sayoko Tanaka ◽  
Hideaki Higashikuze ◽  
Nobuo Ikekawa ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Bile Acids ◽  
Gas Liquid Chromatography ◽  
Human Bile

scholarly journals Measurement of human serum bile acids by gas–liquid chromatography

1965 ◽  
Vol 6 (2) ◽  
pp. 182-192 ◽  
Author(s):  
D.H. Sandberg ◽  
J. Sjövall ◽  
K. Sjövall ◽  
D.A. Turner
Keyword(s):  
Liquid Chromatography ◽  
Human Serum ◽  
Bile Acids ◽  
Gas Liquid Chromatography ◽  
Serum Bile Acids

Carbohydrate Moieties of Glycoproteins in Human Hepatic and Gall-Bladder Bile, Gall-Bladder Mucosa and Gall Stones

1979 ◽  
Vol 56 (6) ◽  
pp. 533-538 ◽  
Author(s):  
S. P. Lee ◽  
T. H. Lim ◽  
A. J. Scott
Keyword(s):  
Liquid Chromatography ◽  
Gall Bladder ◽  
Mixed Micelles ◽  
Gas Liquid Chromatography ◽  
Variable Amount ◽  
Bladder Mucosa ◽  
Human Bile ◽  
Acetylneuraminic Acid ◽  
Gall Stones ◽  
Cholesterol Solubilization

1. The soluble glycoproteins of human bile, gall-bladder mucosa and gall stones have been extracted and hydrolysed, and the monosaccharides analysed by gas-liquid chromatography. 2. Human biliary glycoproteins contained 55–75% of carbohydrate, the major monosaccharide components being galactose, fucose and N-acetylglucosamine, accounting for 70–85% of all the monosaccharides. Mannose, glucose, N-acetylgalactosamine and N-acetylneuraminic acid (sialic acid) were also present. N-Acetylneuraminic acid was present in large amounts in the gall-bladder mucosa and bile of one ulcerated and markedly inflamed gall bladder. 3. The proportion of monosaccharides in soluble glycoproteins of mucosa and bile were not different in samples from subjects with or without gall stones. 4. Gall stones were analysed for cholesterol, calcium and bilirubin and classified as ‘cholesterol stones’ (7/10) and ‘pigment stones’ (3/10). Both cholesterol and pigment stones contain a variable amount of glycoprotein. The pattern of carbohydrate constituents was similar to that present in the gall-bladder mucosa and bile in the same subject. There was also no major difference between the pattern found in ‘cholesterol’ and ‘pigment’ stones. 5. Evidence and argument are presented suggesting that some glycoprotein is secreted by the gall bladder and incorporated into gall stones. This calls for further work upon the influence of these carbohydrate-rich macromolecules on cholesterol solubilization in mixed micelles.

DISULPHATES OF NEUTRAL STEROIDS IN THE AMNIOTIC FLUID FROM ANENCEPHALIC PREGNANCIES

1971 ◽  
Vol 51 (1) ◽  
pp. 109-115 ◽  
Author(s):  
T. LUUKKAINEN ◽  
E. A. MICHIE ◽  
R. VIHKO
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Amniotic Fluid ◽  
Normal Pregnancy ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Human Bile ◽  
Normal Term ◽  
Pregnancy Urine

SUMMARY Steroid disulphate fractions were obtained by chromatography on Sephadex LH-20 from samples of amniotic fluid from three pregnancies complicated by anencephaly. Several of the steroids with a 3β-hydroxy-5-ene structure found in normal amniotic fluid could not be detected. 5-Androstene-3β,17α-diol, the main steroid disulphate in normal amniotic fluid was present in concentrations of <5% of the values at normal term. The disulphates of neutral steroids found in highest concentrations in the amniotic fluid of anencephalic pregnancies were those of isomeric pregnanediols; 5α-pregnane-3α,20α-diol, 5α-pregnane-3β,20α-diol and 5β-pregnane-3α,20α-diol. The quantities of these metabolites of progesterone in the amniotic fluid of anencephalic pregnancies were similar to those in normal pregnancies. A saturated 18-hydroxylated C19 steroid, most probably 3α,18-dihydroxy-5α-androstan-17-one, was identified by gas—liquid chromatography and gas chromatography—mass spectrometry. Although this compound has been previously identified in human bile and in normal pregnancy urine, this appears to be the first identification of a saturated C19 steroid in amniotic fluid.

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