Differential determination of bile acids in human bile with partition chromatography and gas liquid chromatography

1967 ◽  
Vol 2 (1) ◽  
pp. 74-74
Author(s):  
R. Torigoe ◽  
K. Uchida ◽  
Y. Chinen
Keyword(s):  
Liquid Chromatography ◽  
Bile Acids ◽  
Gas Liquid Chromatography ◽  
Partition Chromatography ◽  
Human Bile

scholarly journals Studies on Bile Acids and Bile Alcohols. I. Gas Liquid Chromatography of Human Bile Acids.

1964 ◽  
Vol 12 (6) ◽  
pp. 710-713 ◽  
Author(s):  
Kyosuke Tsuda ◽  
Yoshihiro Sato ◽  
Sayoko Tanaka ◽  
Hideaki Higashikuze ◽  
Nobuo Ikekawa ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Bile Acids ◽  
Gas Liquid Chromatography ◽  
Human Bile

EXCRETION OF BILE ACIDS BY THREE MEN ON A FAT-FREE DIET

1966 ◽  
Vol 44 (6) ◽  
pp. 957-969 ◽  
Author(s):  
S. S. Ali ◽  
A. Kuksis ◽  
J. M. R. Beveridge
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Bile Acids ◽  
The Other ◽  
Total Output ◽  
Gas Liquid Chromatography ◽  
Fecal Bile Acids ◽  
Layer Chromatography ◽  
Total Excretion

A combination of thin-layer chromatography and gas–liquid chromatography was used for the separation and determination of fecal bile acids in three middle-aged men during the last 4 days of three 8-day periods on a fat-free diet. The range of the total output was 130–650 mg/day. Lithocholic and deoxycholic acids were the major components and accounted for 65–80% of the total excretion of bile acids. Other common bile acids were present in considerably smaller quantities (0.5–11% each). In addition, evidence was obtained for the presence of a number of as yet unreported fecal bile acids. These were tentatively identified as 3α,12β-dihydroxy-, 3β,12β-dihydroxy-, 3α,7β-dihydroxy-, 3α-monohydroxy-7-keto-, 12α-monohydroxy-3-keto-, and 7β-monohydroxy-cholanic and cholanic acids. The latter three acids occurred in traces only, but the other uncommon fecal bile acids each accounted for 0.5–7% of the total excretion of bile acids. Significant variations were found in the concentrations of several of the bile acids both between different samples from the same subject and between samples from different subjects.

Determination of serum bile acids in the patients with hepatobiliary diseases with gas liquid chromatography

1967 ◽  
Vol 2 (4) ◽  
pp. 365-366
Author(s):  
I. Makino ◽  
H. Taniguchi ◽  
T. Ishiya ◽  
S. Nakagawa ◽  
K. Mashimo
Keyword(s):  
Liquid Chromatography ◽  
Bile Acids ◽  
Gas Liquid Chromatography ◽  
Serum Bile Acids ◽  
Hepatobiliary Diseases

Determination of Vitamins D2 and D3 in Pharmaceuticals by Gas-Liquid Chromatography

1968 ◽  
Vol 51 (4) ◽  
pp. 839-840
Author(s):  
T K Murray ◽  
P Erdody ◽  
T Panalaks
Keyword(s):  
Vitamin D ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Coefficient Of Variation ◽  
Internal Standard ◽  
Antimony Trichloride ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Partition Chromatography

Abstract A method is described for determining vitamin D in multivitamin preparations; in the method, vitamins D2 and D3 are isomerized with antimony trichloride and separated by GLC. Vitamins D,2 and D3 are differentiated and measured separately and one vitamin may be used as an internal standard for the other. Vitamin A is largely removed by partition chromatography but can be tolerated in the final dilution in a ratio of 1:1 with vitamin D. When the method was used for the assay of multivitamin preparations, the coefficient of variation was 3.2%.

Determination of individual human faecal bile acids by gas-liquid chromatography after enzymatic deconjugation and simultaneous solvolysis and methylation using dimethoxypropane

1985 ◽  
Vol 152 (1-2) ◽  
pp. 231-239 ◽  
Author(s):  
Arendina van Faassen ◽  
Fokko M. Nagengast ◽  
Magda Hectors ◽  
Wim J.M. van den Broek ◽  
Anton W.M. Huijbregts ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Bile Acids ◽  
Gas Liquid Chromatography ◽  
Individual Human
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