scholarly journals Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain

2011 ◽  
Vol 34 (10) ◽  
pp. 1648-1651 ◽  
Author(s):  
Kosuke Nakamura ◽  
Hiroshi Akiyama ◽  
Kiyomi Ohmori ◽  
Yuki Takahashi ◽  
Reona Takabatake ◽  
...  
Keyword(s):  
Detection Method ◽  
Genetically Modified ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

scholarly journals Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Guangyuan Zhao ◽  
Pu Yan ◽  
Wentao Shen ◽  
Decai Tuo ◽  
Xiaoying Li ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Isolate ◽  
Genetically Modified ◽  
Hainan Island ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Rt Pcr ◽  
Sequence Identity

The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.

scholarly journals Geographical and Genetic Divergence Among Papaya ringspot virus Populations Within Hainan Province, China

2016 ◽  
Vol 106 (8) ◽  
pp. 937-944 ◽  
Author(s):  
Hui Zhao ◽  
Rui Zong Jia ◽  
Yu-Liang Zhang ◽  
Yun Judy Zhu ◽  
Hui-Cai Zeng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Geographical Distribution ◽  
Genetic Divergence ◽  
Genetically Modified ◽  
Hainan Island ◽  
Ringspot Virus ◽  
Hainan Province ◽  
Papaya Ringspot Virus ◽  
Frequent Mutations ◽  
Specific Virus

Papaya ringspot virus (PRSV) severely affects the global papaya industry. Transgenic papaya has been proven to have effective resistance to PRSV isolates from Hawaii, Thailand, Taiwan, and other countries. However, those transgenic cultivars failed to show resistance to Hainan Island isolates. Some 76 PRSV samples, representative of all traditional papaya planting areas across five cities (Wen Chang, n = 13; Cheng Mai, n = 14; Chang Jiang, n = 11; Le Dong, n = 25; and San Ya, n = 13) within Hainan Province, were investigated. Results revealed three genetic diversity groups (Hainan I, II, and III) that correlated with geographical distribution. Frequent mutations among PRSV isolates from Hainan were also observed. The high genetic divergence in PRSV isolates from Hainan is likely to be the cause of the failure of genetically modified papaya that targets sequence-specific virus.

Repeated Dose 90-Day Feeding Study of Whole Fruits of Genetically Modified Papaya Resistant to Papaya Ringspot Virus in Rats

2015 ◽  
Vol 63 (4) ◽  
pp. 1286-1292 ◽  
Author(s):  
Hsin-Tang Lin ◽  
Gow-Chin Yen ◽  
Wei-Cheng Lee ◽  
Yi-Ting Tsai ◽  
Jhaol-Huei Wu ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Ringspot Virus ◽  
Repeated Dose ◽  
Papaya Ringspot Virus ◽  
Feeding Study

scholarly journals Identification and Detection of Genetically Modified Papaya Resistant to Papaya Ringspot Virus Strains in Thailand

2014 ◽  
Vol 37 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Kosuke Nakamura ◽  
Kazunari Kondo ◽  
Tomoko Kobayashi ◽  
Akio Noguchi ◽  
Kiyomi Ohmori ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Strains

scholarly journals Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat

2018 ◽  
Vol 14 (3) ◽  
pp. 89
Author(s):  
Irsan Nuhantoro ◽  
Sri Hendrastuti Hidayat ◽  
Kikin Hamzah Mutaqin
Keyword(s):  
Nucleic Acid ◽  
Detection Method ◽  
Blot Hybridization ◽  
High Specificity ◽  
Dna Probe ◽  
Ringspot Virus ◽  
Nucleic Acid Hybridization ◽  
Disease Spread ◽  
Papaya Ringspot Virus ◽  
Dot Blot

Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya. The disease had not been found in Indonesia, until disease outbreak in Nangroe Aceh Darussalam was reported in 2012. Since then, the disease spread rapidly in most papaya growing areas in Sumatera, Java and Bali. Papaya ringspot virus (PRSV) is generally detected using serological or polymerase chain reaction methods. Improvement in detection method is necessary to facilitate a more reliable tool for controlling the spread of PRSV. The aim of the research was to construct DNA probe for development of detection method based on nucleic acid hybridization. Molecular characterization based on HCPro gene sequence indicated high homology (97.88 to 99.05%) among PRSV isolates from Boyolali (Central Java), Medan (North Sumatera), Sleman (Yogyakarta) and Tabanan (Bali). Two DNA clones of HCPro gene were selected for probe construction and the probes were then labeled using PCR DIG-dioxigenin. Optimization of nucleic acid dot blot hybridization method to achieve strongest positive reaction was developed, i.e. using stringency washes at 1×SSC, 0.1% SDS, incubation at 60 oC for 15’. The DNA probe for PRSV has a high specificity and sensitifity; it could detect PRSV at the lowest concentration of nucleic acid (0.062 µg µL-1).

Screening the Watermelon Germplasm Collection for Resistance to Papaya Ringspot Virus Type‐W

Crop Science ◽  
2002 ◽  
Vol 42 (4) ◽  
pp. 1324-1330 ◽  
Author(s):  
E. Bruton Strange ◽  
Nihat Guner ◽  
Zvezdana Pesic‐VanEsbroeck ◽  
Todd C. Wehner
Keyword(s):  
Virus Type ◽  
Germplasm Collection ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

scholarly journals Erratum to: Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates

Virus Genes ◽  
2007 ◽  
Vol 35 (2) ◽  
pp. 431-431
Author(s):  
Juan Carlos Noa-Carrazana ◽  
Diego González-de-León ◽  
Laura Silva-Rosales
Keyword(s):  
Molecular Characterization ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Severe Isolate

Transgene-specific and event-specific molecular markers for characterization of transgenic papaya lines resistant to Papaya ringspot virus

2009 ◽  
Vol 18 (6) ◽  
pp. 971-986 ◽  
Author(s):  
Ming-Jen Fan ◽  
Shu Chen ◽  
Yi-Jung Kung ◽  
Ying-Huey Cheng ◽  
Huey-Jiunn Bau ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Transgenic Papaya

Generation of mild recombinants of papaya ringspot virus to minimize the problem of strain-specific cross protection

2021 ◽  
Author(s):  
Thi-Thu-Yen Tran ◽  
Tzu-Tung Lin ◽  
Chung-Ping Chang ◽  
Chun-Hung Chen ◽  
Van-Hoa Nguyen ◽  
...  
Keyword(s):  
Nitrous Acid ◽  
Carica Papaya ◽  
Genomic Sequence ◽  
Cross Protection ◽  
Ringspot Virus ◽  
Limiting Factor ◽  
Papaya Ringspot Virus ◽  
Severe Damage ◽  
Mild Strain ◽  
Geographic Regions

Papaya ringspot virus (PRSV) causes severe damage to papaya (Carica papaya L.) and is the primary limiting factor for papaya production worldwide. A nitrous acid-induced mild strain PRSV HA 5-1, derived from Hawaii strain HA, has been applied to control PRSV by cross protection for decades. However, the problem of strain-specific protection hampers its application in Taiwan and other geographic regions outside Hawaii. Here, sequence comparison of the genomic sequence of HA 5-1 with that of HA revealed 69 nucleotide changes, resulting in 31 aa changes in which 16 aa are structurally different. The multiple mutations of HA 5-1 are considered resulting from nitrous-acid induction since 86% of nucleotide changes are transition mutations. The stable HA 5-1 was used as a backbone to generate recombinants carrying individual 3’ fragments of Vietnam severe strain TG5, including NIa, NIb, and CP3’ regions, individually or in combination. Our results indicated that the best heterologous fragment for the recombinant is the region of CP3’, with which symptom attenuation of the recombinant is like that of HA 5-1. This mild recombinant HA51/TG5-CP3’ retained high levels of protection against the homologous HA in papaya plants and significantly increased the protection against the heterologous TG-5. Similarly, HA 5-1 recombinants carrying individual CP3’ fragments from Thailand SMK, Taiwan YK, and Vietnam ST2 severe strains also significantly increase the protection against the corresponding heterologous strains in papaya plants. Thus, our recombinant approach for mild strain generation is a fast and effective way to minimize the problem of strain-specific protection.

scholarly journals Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

2009 ◽  
Vol 35 (3) ◽  
pp. 223-225 ◽  
Author(s):  
José Segundo Giampan ◽  
Jorge Alberto Marques Rezende ◽  
Sônia Maria De Stefano Piedade
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

O ZLCV é um tospovírus encontrado com freqüência causando severos danos em cucurbitáceas. Nesse trabalho avaliaram-se os danos causados pelo ZLCV em abobrinha de moita 'Caserta', em campo na ESALQ/USP, Piracicaba-SP, onde esse vírus é freqüente. Plantas obtidas pela semeadura direta foram monitoradas periodicamente quanto à infecção pelo ZLCV por meio dos sintomas e por PTA-ELISA. Monitorou-se ainda a contaminação com Papaya ringspot virus - type W e Zucchini yellow mosaic virus, desconsiderando a produção dessas plantas. As plantas foram agrupadas em função da época de aparecimento dos sintomas do ZLCV, avaliando a produção de frutos comerciais (FC) e não comerciais (FNC) de cada grupo e comparando com a de plantas que permaneceram sem sintomas até o final do experimento. As plantas que apresentaram sintomas até os 23 dias após a emergência (DAE) não produziram qualquer tipo de frutos. FC foram colhidos de plantas que apresentaram sintomas a partir dos 42 DAE. Mesmo assim, houve redução de 78,5 % na produção de FC. Plantas que mostraram sintomas por ocasião da última colheita (55 DAE) apresentaram redução na produção de FC de 9,6 %. A infecção com o ZLCV até o início da frutificação inviabiliza a produção de FC de abobrinha de moita 'Caserta'.

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