scholarly journals Multiple Target Cell Extraction and LC-MS Analysis for Predicting Bioactive Components in Radix Salviae Miltiorrhizae

2008 ◽  
Vol 31 (3) ◽  
pp. 501-506 ◽  
Author(s):  
Fan-Na Qu ◽  
Lian-Wen Qi ◽  
Ying-Jie Wei ◽  
Xiao-Dong Wen ◽  
Ling Yi ◽  
...  
Keyword(s):  
Target Cell ◽  
Multiple Target ◽  
Bioactive Components ◽  
Radix Salviae Miltiorrhizae ◽  
Cell Extraction ◽  
Salviae Miltiorrhizae ◽  
Ms Analysis

scholarly journals Rapid identification of direct-acting pancreatic protectants from Cyclocarya paliurus leaves tea by the method of serum pharmacochemistry combined with target cell extraction

2020 ◽  
Vol 18 (1) ◽  
pp. 265-274
Author(s):  
Wei-hong Chen ◽  
Zhen Luo ◽  
Zi-Wan Ning ◽  
Jiao Peng ◽  
Xiao-peng Hu ◽  
...  
Keyword(s):  
Target Cell ◽  
Rapid Identification ◽  
Target Cells ◽  
Bioactive Components ◽  
Β Cell ◽  
Cyclocarya Paliurus ◽  
Cell Extraction ◽  
Direct Acting ◽  
Apoptotic Effects ◽  
Tof Ms

AbstractExtracts of Cyclocarya paliurus (CP) leaves, a popular sweet tea, inhibit pancreatic β cell apoptosis and have potent hypoglycemic effects, but the identities of the anti-apoptotic bioactive components are still unknown. In the present study, a method using UPLC-Q-TOF/MS based on serum pharmacochemistry combined with target cell extraction was established to rapidly identify direct-acting pancreatic protectants from CP. After orally administering a set amount of CP extract to rats, blood samples were collected to characterize the components that can be absorbed into the blood using UPLC-Q-TOF/MS. Also, target cells (pancreatic β NIT-1 cells) were incubated with CP extract for 24 hours, and cells were collected to identify the components that can bind to the cells using UPLC-Q-TOF/MS. Finally, to evaluate the protective effect of the bioactive components of CP, MTT and TUNEL assays were performed on treated NIT-1 cell induced by streptozotocin (STZ). Three potential direct-acting pancreatic protectants -- kaempferol, quercetin, quadranoside IV -- were identified, and anti-apoptotic effects of kaempferol and quercetin were confirmed in STZ-induced NIT-1 cells. The findings indicate that this combined approach is a feasible, rapid, and expedient tool for capturing potential direct-acting components from natural products such as those from CP leaves.

Improved stability of salvianolic acid B from Radix Salviae miltiorrhizae in deep eutectic solvents

2016 ◽  
Vol 8 (11) ◽  
pp. 2502-2509 ◽  
Author(s):  
Jue Chen ◽  
Shi-fei Li ◽  
Zhi-feng Yao ◽  
Da-wei Yang ◽  
Li-wei Zhang
Keyword(s):  
Environmentally Friendly ◽  
Deep Eutectic Solvents ◽  
Salvianolic Acid B ◽  
Radix Salviae Miltiorrhizae ◽  
Salvianolic Acid ◽  
Salviae Miltiorrhizae ◽  
Improved Stability ◽  
The Stability

Environmentally friendly deep eutectic solvents were developed for enhancing the stability of salvianolic acid B from Radix Salviae miltiorrhizae.

scholarly journals Simultaneous Determination and Pharmacokinetic Comparisons of Multi-Ingredients after Oral Administration ofRadix Salviae MiltiorrhizaeExtract, Hawthorn Extract, and a Combination of Both Extracts to Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Qiang Liu ◽  
Qian Cai ◽  
Chang Liu ◽  
Feng-Wei Bao ◽  
Zhen-Qiu Zhang
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rosmarinic Acid ◽  
Rat Plasma ◽  
Hplc Method ◽  
Radix Salviae Miltiorrhizae ◽  
Salviae Miltiorrhizae ◽  
Hawthorn Extract

A simple and sensitive HPLC method was developed for simultaneous determination of danshensu (DSS), rosmarinic acid (RA), lithospermic acid (LA), salvianolic acid B (SAB), and hyperoside (HP) in rat plasma. This method validated was successfully applied to the pharmacokinetic study of the main active ingredients after oral administration ofRadix Salviae Miltiorrhizaeextract (SME), hawthorn extract (HTE), and a combination of both extracts (2.5 : 1) to rats. The results indicated that there have been great differences in pharmacokinetics between a single extract and a combination of both extracts. A combination of both extracts can enhance their bioavailabilities and delay the elimination of SAB and DSS in rats.

Sign in / Sign up

Export Citation Format

Share Document