Target cell extraction coupled with LC-MS/MS analysis for screening potential bioactive components inGinkgo bilobaextract with preventive effect against diabetic nephropathy

2014 ◽  
Vol 29 (2) ◽  
pp. 226-232 ◽  
Author(s):  
Jing-ying Qiu ◽  
Xu Chen ◽  
Xiao-xiao Zheng ◽  
Xiang-lan Jiang ◽  
Dong-zhi Yang ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Target Cell ◽  
Bioactive Components ◽  
Preventive Effect ◽  
Cell Extraction ◽  
Ms Analysis

scholarly journals Rapid identification of direct-acting pancreatic protectants from Cyclocarya paliurus leaves tea by the method of serum pharmacochemistry combined with target cell extraction

2020 ◽  
Vol 18 (1) ◽  
pp. 265-274
Author(s):  
Wei-hong Chen ◽  
Zhen Luo ◽  
Zi-Wan Ning ◽  
Jiao Peng ◽  
Xiao-peng Hu ◽  
...  
Keyword(s):  
Target Cell ◽  
Rapid Identification ◽  
Target Cells ◽  
Bioactive Components ◽  
Β Cell ◽  
Cyclocarya Paliurus ◽  
Cell Extraction ◽  
Direct Acting ◽  
Apoptotic Effects ◽  
Tof Ms

AbstractExtracts of Cyclocarya paliurus (CP) leaves, a popular sweet tea, inhibit pancreatic β cell apoptosis and have potent hypoglycemic effects, but the identities of the anti-apoptotic bioactive components are still unknown. In the present study, a method using UPLC-Q-TOF/MS based on serum pharmacochemistry combined with target cell extraction was established to rapidly identify direct-acting pancreatic protectants from CP. After orally administering a set amount of CP extract to rats, blood samples were collected to characterize the components that can be absorbed into the blood using UPLC-Q-TOF/MS. Also, target cells (pancreatic β NIT-1 cells) were incubated with CP extract for 24 hours, and cells were collected to identify the components that can bind to the cells using UPLC-Q-TOF/MS. Finally, to evaluate the protective effect of the bioactive components of CP, MTT and TUNEL assays were performed on treated NIT-1 cell induced by streptozotocin (STZ). Three potential direct-acting pancreatic protectants -- kaempferol, quercetin, quadranoside IV -- were identified, and anti-apoptotic effects of kaempferol and quercetin were confirmed in STZ-induced NIT-1 cells. The findings indicate that this combined approach is a feasible, rapid, and expedient tool for capturing potential direct-acting components from natural products such as those from CP leaves.

Finding diabetic nephropathy biomarkers in the plasma peptidome by high-throughput magnetic bead processing and MALDI-TOF-MS analysis

2010 ◽  
Vol 4 (8-9) ◽  
pp. 697-705 ◽  
Author(s):  
Henning G. Hansen ◽  
Julie Overgaard ◽  
Maria Lajer ◽  
Frantisek Hubalek ◽  
Peter Højrup ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
High Throughput ◽  
Magnetic Bead ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

GC–MS analysis of bioactive components and synthesis of gold nanoparticle using Chloroxylon swietenia DC leaf extract and its larvicidal activity

2015 ◽  
Vol 148 ◽  
pp. 1-8 ◽  
Author(s):  
Govindasamy Balasubramani ◽  
Rajendiran Ramkumar ◽  
Narayanaswamy Krishnaveni ◽  
Rajamani Sowmiya ◽  
Paramasivam Deepak ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Larvicidal Activity ◽  
Leaf Extract ◽  
Bioactive Components ◽  
Ms Analysis ◽  
Chloroxylon Swietenia

GC-MS Analysis and Molecular Docking of Bioactive Components from Leaves of Guaiacum officinale for Anti-inflammatory Activity

2019 ◽  
Vol 4 (3) ◽  
pp. 180-184
Author(s):  
Vijetha Pendyala ◽  
Vidyadhara Suryadevara ◽  
Subbarao Jampani ◽  
Suhasini Jala
Keyword(s):  
Molecular Docking ◽  
Phospholipase A2 ◽  
Retention Time ◽  
Docking Studies ◽  
Bioactive Components ◽  
Molecular Docking Studies ◽  
Ms Analysis ◽  
Cox 2 ◽  
Guaiacum Officinale ◽  
Interleukin Receptor

Present study was an attempt to investigate the bioactive components present in the leaves of Guaiacum officinale using gas chromatography-mass spectroscopy (GC-MS) analysis and study the antiinflammatory potential of those constituents using molecular docking studies. GC-MS analysis was done by standard protocol using the equipment JEOL GC MATE II. The identification of components was based on NIST (National Institute of Standards and Technology) Version-11 library as well as comparison of their retention indices. The molecular docking studies were done using the commercial docking software MCULE, 1-click docking. GC-MS analysis of the alcoholic extract showed the presence of ten compounds at different retention times. The phytoconstituent 8,11,14-eicosatrienoic acid was present at high concentration with % peak area of 43.3 at a retention time of 19.43 min followed by 5,7-dihydroxy-8-methoxy flavone (Wogonin) at a retention time of 17.73. All 10 compounds obtained from GC-MS analysis and diclofenac were used as the ligands in this study, with cyclooxygenase-2 (COX-2), phospholipase A2 and interleukin receptor as the molecular targets. in silico Docking studies revealed that the flavanoid Wogonin is having highest binding potential indicated by least docking score of -8.2, -8 and -6.9 kcal/mol on COX-2, phospholipase A2 and interleukin receptor respectively.

scholarly journals Preventive effect of taurine on experimental type II diabetic nephropathy

2010 ◽  
Vol 17 (Suppl 1) ◽  
pp. S46 ◽  
Author(s):  
Shumei Lin ◽  
Jiancheng Yang ◽  
Gaofeng Wu ◽  
Mei Liu ◽  
Xinhong Luan ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Type Ii ◽  
Preventive Effect ◽  
Experimental Type

Py-GC/MS Analysis of Bioactive Components of 450°C Pyrolyzate from Ethanol Extractives of Oil-Tea Cake

2011 ◽  
Vol 480-481 ◽  
pp. 513-518
Author(s):  
Qi Mei Liu ◽  
Wan Xi Peng
Keyword(s):  
Phosphonic Acid ◽  
Value Added ◽  
Bioactive Components ◽  
Cyclobutanedicarboxylic Acid ◽  
Ms Analysis ◽  
Cosmetic Industry ◽  
Analytical Result ◽  
Main Components

450°C-based pyrolysis- GC/MS technology was used to analyze the bioactive components of ethanol extractives of oil-tea cake. The analytical result showed that 38 peaks were obtained from the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake, and 37 compounds representing 97.81 % of the total areas were identified (Table 1). The analytical result showed that the main components of ethanol extractives of oil-tea cake by 450°C-based pyrolysis- GC/MS were as: Benzene, 1,2,3-Trimethoxy-5-Methyl- (21.56%), Phenol, 3,4-Dimethoxy- (16.31%), 3',5'-Dimethoxyacetophenone (14.37%), 1,2-Ethanediamine, N-Ethyl- (6.62%), Indole (5.39%), Mequinol (4.13%), Phosphonic Acid, (P-Hydroxyphenyl)- (2.97%), 1,2-Cyclobutanedicarboxylic Acid, Cis- (1.67%), Bicyclo 3.1.0 Hexan-3-One (1.38%), Benzene, 1,4-Dimethoxy-2,3,5,6- Tetramethyl- (1.35%), 2h-Azepin-2-One, Hexahydro-1-(2-Propenyl)- (1.15), etc. The results of function analyses showed that the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake can be used as rare natural medicinal materials, and value-added materials of spice, food and cosmetic industry.

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