scholarly journals Development of an Assay System for Saikosaponin a Using Anti-saikosaponin a Monoclonal Antibodies

2004 ◽  
Vol 27 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Shu-hang Zhu ◽  
Shin-ichi Shimokawa ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama
Keyword(s):  
Monoclonal Antibodies ◽  
Assay System ◽  
Saikosaponin A

A simple assay system incorporating monoclonal antibodies for the detection of potato virus T

1993 ◽  
Vol 36 (2) ◽  
pp. 83-88 ◽  
Author(s):  
Mairi L. Vernon-Shirley ◽  
Robert Burns ◽  
Edna L. George ◽  
Margaret E. Hoadley
Keyword(s):  
Monoclonal Antibodies ◽  
Potato Virus ◽  
Assay System

A Decade of Development in Immunoassay Methodology

1990 ◽  
Vol 36 (8) ◽  
pp. 1408-1427 ◽  
Author(s):  
James P Gosling
Keyword(s):  
Monoclonal Antibodies ◽  
Liquid Phase ◽  
Analytical Methods ◽  
Clinical Laboratory ◽  
Solid Phase ◽  
Detection Limits ◽  
Assay System ◽  
Future Trends ◽  
Homogeneous Systems ◽  
Major Trend

Abstract Immunoassays are now very widely used in the clinical laboratory, either because no other type of assay system is feasible or because they are often the most effective and suitable of the possible analytical methods. The last decade has seen the development and refinement of many new immunoassay reagents and systems. The major trend has been away from liquid-phase assays involving radioisotopic labels, towards fast homogeneous or solid-phase assays capable of operation anywhere; and towards precise and reliable nonisotopic, automated or semi-automated laboratory assays, often with detection limits measured in pico- or attomoles. The use of monoclonal antibodies is now widespread, and the methodologies of labels and of solid-phase components are much more sophisticated. New assay formulations, novel homogeneous systems, immunosensors, free-analyte assays, the importance of thorough validation and of interfering substances, and future trends are discussed.

Development of a canine trypsin-like immunoreactivity assay system using monoclonal antibodies

2002 ◽  
Vol 87 (1-2) ◽  
pp. 41-49 ◽  
Author(s):  
Takaki Waritani ◽  
Yoko Okuno ◽  
Yoshinori Ashida ◽  
Masaharu Hisasue ◽  
Ryo Tsuchiya ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Assay System

Two-Site Immunoradiometric Assay of Intact Salmon Calcitonin

1992 ◽  
Vol 38 (11) ◽  
pp. 2284-2286 ◽  
Author(s):  
L J Deftos
Keyword(s):  
Monoclonal Antibodies ◽  
Detection Limit ◽  
Incubation Time ◽  
Salmon Calcitonin ◽  
Assay System ◽  
The Other ◽  
Polystyrene Bead ◽  
Immunoradiometric Assay ◽  
Peptide Fragments ◽  
Assay Format

Abstract We developed a two-site immunoradiometric assay (IRMA) of salmon calcitonin (SCT) that detects intact SCT(1-32) and not peptide fragments of the hormone. This was accomplished by using monoclonal antibodies prepared against the peptide fragments SCT(1-11) and SCT(11-32). Two antibodies with specificity for each of the peptides were purified from their respective ascites and evaluated in a two-site format wherein one of the antibodies was adsorbed to polystyrene beads and the other was radioiodinated. In this assay format, the antibody pair detected intact SCT(1-32) but did not react with either SCT(1-11) or SCT(11-32). The sensitivity of the assay could be increased by exchanging the antibodies with respect to bead adsorption and radioiodination. Furthermore, by increasing the incubation time and volume of incubation of sample with the polystyrene-bead-adsorbed antibody, the effective detection limit of the assay could be improved. This assay system can be used to detect intact SCT when the presence of fragments of the hormone might otherwise complicate interpretation of assay data.

Eastern blotting and use of anti-saikosaponin a monoclonal antibodies for detection of saikosaponins

2006 ◽  
Vol 61 (2) ◽  
pp. 178-183 ◽  
Author(s):  
Shu-hang Zhu ◽  
Osamu Morinaga ◽  
Shinichi Shimokawa ◽  
Tae Kwon Shon ◽  
Sang Chul Lee ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Eastern Blotting ◽  
Saikosaponin A

The Stratus immunofluorometric assay system evaluated for quantifying human choriogonadotropin in serum.

1986 ◽  
Vol 32 (7) ◽  
pp. 1402-1404 ◽  
Author(s):  
L C Rogers ◽  
S E Kahn ◽  
T H Oeser ◽  
E W Bermes
Keyword(s):  
Monoclonal Antibodies ◽  
Regression Equation ◽  
Assay System ◽  
Beta Subunit ◽  
The Other ◽  
Alpha Subunit ◽  
Human Choriogonadotropin ◽  
Beta Hcg

Abstract We evaluated the Stratus (American Dade, Miami, FL), an automated immunofluorometric assay system, for the quantification of human choriogonadotropin (hCG) in serum or plasma. The assay is based on the "sandwich" (two-site) immunoassay methodology: use of two monoclonal antibodies, one specific for the alpha subunit and the other for the beta subunit, results in an assay that is specific for the intact hCG molecule. Results for the first sample are obtained in 7 min; subsequent additional values are produced at 1-min intervals. Inter-run precision (CV), estimated from replicate determinations of sera, was 4.5% at an hCG concentration of 38 int. units/L, 4.9% at 114, and 6.1% at 194. Intrarun CV was less than 2% at all three concentrations. Correlations of results for 127 specimens analyzed in duplicate with the Stratus (y) and by a radioimmunoassay (x) for beta hCG (Gamma Dab M [cf931125I] beta-hCG, Travenol-Genentech Diagnostics, Cambridge, MA) yielded the following regression equation: y = 0.969x - 6.0 (r = 0.995). The Stratus immunofluorometric system provides a rapid and convenient assay of hCG in serum or plasma.

Ultrastructural analysis of unique glycoconjugates in the rat olfactory system

1992 ◽  
Vol 50 (1) ◽  
pp. 890-891
Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Olfactory System ◽  
Olfactory Bulbs ◽  
Temporal And Spatial Patterns ◽  
Carbohydrate Epitopes ◽  
Olfactory Systems ◽  
Temporal And Spatial ◽  
Cell Surface Glycoconjugates

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.

Structural and Chemical Studies of Pathological Fibers in Human Neurofibrillary Degeneration

1985 ◽  
Vol 43 ◽  
pp. 726-729
Author(s):  
K.S. Kosik ◽  
L.K. Duffy ◽  
S. Bakalis ◽  
C. Abraham ◽  
D.J. Selkoe
Keyword(s):  
Monoclonal Antibodies ◽  
Alzheimer Disease ◽  
Human Brain ◽  
Neurofibrillary Tangles ◽  
Morphological Analysis ◽  
High Specificity ◽  
Cytoskeletal Proteins ◽  
Neuritic Plaque ◽  
Protein Chemistry ◽  
Chemical Studies

The major structural lesions of the human brain during aging and in Alzheimer disease (AD) are the neurofibrillary tangles (NFT) and the senile (neuritic) plaque. Although these fibrous alterations have been recognized by light microscopists for almost a century, detailed biochemical and morphological analysis of the lesions has been undertaken only recently. Because the intraneuronal deposits in the NFT and the plaque neurites and the extraneuronal amyloid cores of the plaques have a filamentous ultrastructure, the neuronal cytoskeleton has played a prominent role in most pathogenetic hypotheses.The approach of our laboratory toward elucidating the origin of plaques and tangles in AD has been two-fold: the use of analytical protein chemistry to purify and then characterize the pathological fibers comprising the tangles and plaques, and the use of certain monoclonal antibodies to neuronal cytoskeletal proteins that, despite high specificity, cross-react with NFT and thus implicate epitopes of these proteins as constituents of the tangles.

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