Concentration of a potent calcium oxalate monohydrate crystal growth inhibitor in the urine of normal persons and kidney stone patients by ELISA-based assay system employing monoclonal antibodies

2003 ◽  
Vol 90 (6) ◽  
pp. 1261-1275 ◽  
Author(s):  
Mehdi F. Moghadam ◽  
C. Tandon ◽  
S. Aggarwal ◽  
S.K. Singla ◽  
S.K. Singh ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Crystal Growth ◽  
Calcium Oxalate ◽  
Kidney Stone ◽  
Growth Inhibitor ◽  
Calcium Oxalate Monohydrate ◽  
Assay System

Isolation of calcium oxalate crystal growth inhibitor from rat kidney and urine

1984 ◽  
Vol 247 (5) ◽  
pp. F765-F772 ◽  
Author(s):  
Y. Nakagawa ◽  
V. Abram ◽  
F. L. Coe
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Column Chromatography ◽  
Dissociation Constants ◽  
Rat Kidney ◽  
Deae Cellulose ◽  
Growth Inhibitor ◽  
Calcium Oxalate Monohydrate ◽  
Heat Denaturation ◽  
Kidney Homogenate

Glycoproteins that slow the growth rate of calcium oxalate monohydrate crystals were purified from rat kidney homogenate and urine by selective heat denaturation (for rat kidney homogenate), DEAE-cellulose column chromatography, and gel permeation column chromatography. Both kidney and urine inhibitors were glycoproteins with an apparent mol wt of 1.4 X 10(4), as determined by high-performance liquid chromatography. They contained gamma-carboxyglutamic acid and a high percentage of aspartic acid and glutamic acid but had few aromatic amino acid residues. Both inhibitors contained fucose, mannose, glucose, galactose, glucosamine, galactosamine, and N-acetylneuraminic acid but no glucuronic acid. Kinetic studies suggest that purified inhibitors bind to calcium oxalate monohydrate seed crystals according to a Langmuir adsorption isotherm with similar dissociation constants of 14 X 10(-8)M for rat urine inhibitor and 8.7 X 10(-8) M for rat kidney inhibitor. The isolation of similar glycoproteins from kidney and urine suggests that urinary crystal growth inhibitor may be produced in the kidneys.

Surface effects in the crystal growth of calcium oxalate monohydrate

1987 ◽  
Vol 118 (2) ◽  
pp. 379-386 ◽  
Author(s):  
R.P. Singh ◽  
S.S. Gaur ◽  
D.J. White ◽  
G.H. Nancollas
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Surface Effects ◽  
Calcium Oxalate Monohydrate

Exploring the Molecular Level Interaction of Human Serum Albumin with Calcium Oxalate Monohydrate Crystals

2021 ◽  
Vol 28 ◽  
Author(s):  
Priyadarshini ◽  
Abhishek Negi ◽  
Chetna Faujdar ◽  
Lokesh Nigam ◽  
Naidu Subbarao
Keyword(s):  
Amino Acids ◽  
Crystal Growth ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Calcium Oxalate ◽  
Human Serum ◽  
Stone Formation ◽  
Calcium Oxalate Monohydrate ◽  
Calcium Oxalate Crystallization ◽  
In Silico Docking

Background: Human serum albumin (HSA) is one of the most abundant proteins in the blood plasma, urine as well as in the organic matrix of renal calculi. Macromolecules present in the urine modulate kidney stone formation either by stimulating or inhibiting crystallization process. Objective: In the present study, effect of HSA protein on the growth of calcium oxalate monohydrate crystal (COM) was investigated. Methods: Crystal growth assay was used to measure oxalate depletion in the crystal seeded solution in the presence of HSA. HSA concentrations exhibiting effect on crystal growth were selected for FTIR and XRD analysis. In silico docking was performed on seven different binding sites of HSA. Results: Albumin is playing dual role in growth of calcium oxalate crystallization. FTIR and XRD studies further revealed HSA exerted strain over crystal thus affecting its structure by interacting with amino acids of its pocket 1. Docking results indicate that out of 7 binding pocket in protein, calcium oxalate interacts with Arg-186 and Lys-190 amino acids of pocket 1. Conclusion: Our study confirms the role of HSA in calcium oxalate crystallization where acidic amino acids arginine and lysine are binding with COM crystals, revealing molecular interaction of macromolecule and crystal in urolithiasis.

Epitaxial Relationships in Urolithiasis: The Brushite—Whewellite System

1977 ◽  
Vol 52 (2) ◽  
pp. 143-148 ◽  
Author(s):  
J. L. Meyer ◽  
J. H. Bergert ◽  
L. H. Smith
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Calcium Oxalate Monohydrate ◽  
Supersaturated Solution ◽  
Scanning Electron Micrographs ◽  
Phosphate Dihydrate ◽  
Acid Environment ◽  
Time Required ◽  
Insight Into ◽  
Scanning Electron

1. Whewellite (calcium oxalate monohydrate) crystals were found to induce epitaxially the heterogeneous nucleation of brushite (calcium monohydrogen phosphate dihydrate) from its metastable supersaturated solution in approximately one-quarter of the time required for spontaneous precipitation in the absence of added nucleating agents. Scanning electron-microscope observation of the crystalline phase showed brushite crystals originating from the whewellite seed crystals. 2. Crystal growth, upon nucleation, proceeded rapidly, and the metastable solutions quickly approached saturation. 3. Brushite crystals also induced the precipitation of calcium oxalate crystals in about one-quarter of the time required for spontaneous precipitation; however, the rate of crystal growth was considerably slower. In support of the chemical data, scanning electron micrographs showed few crystals of calcium oxalate nucleated on the surface of the brushite seed. 4. The results provide some insight into the cause of stones containing calcium oxalate or calcium phosphate (or both), which form in the normally acid environment of human urine.

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