scholarly journals Estimation of Estrogenic and Anti-estrogenic Activities of Some Phthalate Diesters and Monoesters by MCF-7 Cell Proliferation Assay in Vitro

2003 ◽  
Vol 26 (8) ◽  
pp. 1219-1224 ◽  
Author(s):  
Tomoko Okubo ◽  
Toshinari Suzuki ◽  
Yoshiko Yokoyama ◽  
Kazutaka Kano ◽  
Itsu Kano
Keyword(s):  
Cell Proliferation ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Mcf 7

scholarly journals Analysis of Estrogenic Activity in Maryland Coastal Bays Using the MCF-7 Cell Proliferation Assay

2021 ◽  
Vol 18 (12) ◽  
pp. 6254
Author(s):  
Rehab Elfadul ◽  
Roman Jesien ◽  
Ahmed Elnabawi ◽  
Paulinus Chigbu ◽  
Ali Ishaque
Keyword(s):  
Cell Proliferation ◽  
Estrogenic Activity ◽  
Landing Site ◽  
Negative Control ◽  
Proliferation Assay ◽  
Negative Effects ◽  
Cell Proliferation Assay ◽  
Coastal Bays ◽  
Maryland Coastal Bays ◽  
Mcf 7

Contaminants of Emerging Concern (CECs) with estrogenic or estrogenic-like activity have been increasingly detected in aquatic environments and have been an issue of global concern due to their potential negative effects on wildlife and human health. This study used the MCF-7 cell proliferation assay (E-Screen) to assess the estrogenic activity profiles in Maryland Coastal Bays (MCBs), a eutrophic system of estuaries impacted by human activities. Estrogenic activity was observed in all study sites tested. Water samples from MCBs increased MCF-7 cell proliferation above the negative control from 2.1-fold at site 8, located in Sinepuxent Bay close to the Ocean City Inlet, to 6.3-fold at site 6, located in Newport Bay. The proliferative effects of the sediment samples over the negative control ranged from 1.9-fold at the Assateague Island National Seashore site to 7.7-fold at the Public Landing site. Moreover, elevated cell proliferation (p < 0.05) was observed when cells were co-exposed with 17ß-Estradiol (E2), while reduction in cell proliferation was observed when cells were co-exposed with the antagonist ICI 182, 780 suggesting that cell proliferative effects were primarily mediated by the estrogen receptor (ER). These results suggest the occurrence of some estrogenic or hormonal-like compounds in the MCBs and are consistent with our previous findings based on vitellogenin analyses.

scholarly journals A Robotic MCF-7:WS8 Cell Proliferation Assay to Detect Agonist and Antagonist Estrogenic Activity

2013 ◽  
Vol 137 (2) ◽  
pp. 335-349 ◽  
Author(s):  
C. Z. Yang ◽  
W. Casey ◽  
M. A. Stoner ◽  
G. J. Kollessery ◽  
A. W. Wong ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogenic Activity ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Agonist And Antagonist ◽  
Mcf 7

Adrenomedullin and its receptors expression in renal tumors

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 10568-10568
Author(s):  
J. Deville ◽  
S. Salas ◽  
C. Bartoli ◽  
C. Dupuis ◽  
F. Duffaud ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Collecting Duct ◽  
Significant Inhibition ◽  
Renal Tumors ◽  
Strong Positive Correlation ◽  
Proliferation Assay ◽  
Fuhrman Grade ◽  
Cell Proliferation Assay

10568 Background: Adrenomedullin (AM) is a 52 amino acid peptide that has an important role on tumor cell proliferation and neoangiogenesis through its receptors CRLR/RAMP2 and CRLR/RAMP3. AM gene expression is stimulated by Hypoxia Inductible Factor-1 (HIF- 1) and 60–80% of the human conventional renal carcinomas (cRCC) display mutations in the tumor suppressor protein von Hippel-Lindau, which leads to constitutively elevated HIF-1. Methods: Tumors and non-malignant kidney tissues were obtained from patients who underwent unilateral nephrectomy. We studied VEGFA, AM and its receptors expression by quantitative RT-PCR in 62 frozen renal tumors including: 40 cRCC; 5 cRCC metastasis; 5 chromophobe carcinomas; 5 Papillary carcinomas; 2 oncocytomas; 2 collecting duct carcinomas; 2 normal adjacent renal tissue; 1 unclassified renal tumor. AM and AM-receptors expression was studied in 42 paraffin-embedded renal tumors by immunohistochemistry using rabbit anti-AM, anti-CRLR, anti-RAMP2 and anti-RAMP3 polyclonal antibodies. Effects of these anti AM or anti AM- receptors antibodies on cell proliferation were examined in vitro on BIZ cell line (cRCC cells). Results: VEGFA, AM and AM-receptors genes were overexpressed in cRCC compared to other renal tumors (Except AM gene in chromophobe carcinoma). Their expressions were independent of classical prognostic factors (such as Fuhrman Grade and pT status). In cRCC, there was a strong positive correlation between VEGF-A gene expression and AM (r=0.7; p=0.01) and AM-receptors genes expressions (RAMP2 r=0.61; p= 0.01 and RAMP3 r=0.58; p= 0.01). Immunohistochemistry confirmed a tumor expression of CRLR, RAMP2 and AM in more than 80% cRCC. RAMP3 was expressed by inflammatory cells but not by tumoral cells. The cell proliferation assay showed a significant inhibition of BIZ cell proliferation by AM antibody or AM-receptors antibodies. Conclusion: AM and its receptor CRLR/RAMP2 are overexpressed in cRCC. In vitro cell proliferation assay results support that AM inhibition may suppress cRCC growth, independently of potential antiangiogenic effects. Further studies on animal models are needed but AM pathway may be a potential therapeutic target in cRCC. No significant financial relationships to disclose.

Screening of Korean edible wild plants for estrogenic activity using MCF-7 cell proliferation assay

2011 ◽  
Vol 20 (3) ◽  
pp. 829-833 ◽  
Author(s):  
Young Min Lee ◽  
Ho Young Jung ◽  
Ji Hyun Bae ◽  
Jin Young Lee ◽  
Hwan Hee Jang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogenic Activity ◽  
Wild Plants ◽  
Proliferation Assay ◽  
Cell Proliferation Assay ◽  
Edible Wild Plants ◽  
Mcf 7

scholarly journals In Vitro Cell Proliferation Assay Method Using Rat Gastric Cultured Cells and Effect of Anti-ulcer Drugs on the Proliferation of Cultured Cells

1994 ◽  
Vol 114 (5) ◽  
pp. 316-324 ◽  
Author(s):  
Keiji IMAI ◽  
Reiko UKAI ◽  
Kazuhiro ISHIKAWA ◽  
Hiromoto AOKI ◽  
Tetsuo KATO ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cultured Cells ◽  
Assay Method ◽  
Proliferation Assay ◽  
Cell Proliferation Assay

scholarly journals Evaluation of Manuka Honey Estrogen Activity Using the MCF-7 Cell Proliferation Assay

2016 ◽  
Vol 10 (3) ◽  
pp. 1-11
Author(s):  
Kathleen Henderson ◽  
Tahrir Aldhirgham ◽  
Poonam Nigam ◽  
Richard Owusu-Apenten
Keyword(s):  
Cell Proliferation ◽  
Proliferation Assay ◽  
Manuka Honey ◽  
Cell Proliferation Assay ◽  
Estrogen Activity ◽  
Mcf 7
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