scholarly journals Cloning and Sequencing of the astA Gene Encoding Arylsulfate Sulfotransferase from Salmonella typhimurium.

2001 ◽  
Vol 24 (5) ◽  
pp. 570-574 ◽  
Author(s):  
Jin-Wook KANG ◽  
Ae-Ran KWON ◽  
Dong-Hyun KIM ◽  
Eung-Chil CHOI
Keyword(s):  
Salmonella Typhimurium ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Arylsulfate Sulfotransferase ◽  
Asta Gene

Cloning and sequencing of the meIB gene encoding the melibiose permease of Salmonella typhimurium LT2

1992 ◽  
Vol 234 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Kaori Mizushima ◽  
Shuji Awakihara ◽  
Masayuki Kuroda ◽  
Tetsuya Ishikawa ◽  
Masaaki Tsuda ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Cloning And Sequencing ◽  
Gene Encoding

scholarly journals Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA).

1986 ◽  
Vol 261 (32) ◽  
pp. 14929-14935
Author(s):  
J W Chase ◽  
B A Rabin ◽  
J B Murphy ◽  
K L Stone ◽  
K R Williams
Keyword(s):  
Escherichia Coli ◽  
Large Subunit ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Exonuclease Vii

scholarly journals The Synechococcus Strain PCC 7942glnN Product (Glutamine Synthetase III) Helps Recovery from Prolonged Nitrogen Chlorosis

2000 ◽  
Vol 182 (19) ◽  
pp. 5615-5619 ◽  
Author(s):  
Jörg Sauer ◽  
Ulrike Dirmeier ◽  
Karl Forchhammer
Keyword(s):  
Glutamine Synthetase ◽  
Transcriptional Start Site ◽  
Binding Motif ◽  
Class Iii ◽  
Wild Type ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Low Concentrations ◽  
Synechococcus Strain ◽  
Pcc 7942

ABSTRACT We report the cloning and sequencing of the glnN gene encoding a class III glutamine synthetase from the cyanobacteriumSynechococcus strain PCC 7942. Mapping of the transcriptional start site revealed a DNA sequence in the promoter region that resembles an imperfect NtcA binding motif. Expression ofglnN is impaired in NtcA- and PII-deficient mutants. The only parameter which was negatively affected in theglnN mutant compared to the wild type was the recovery rate of prolonged nitrogen-starved cells with low concentrations of combined nitrogen.

scholarly journals A Novel Gene Encoding Xanthan Lyase of Paenibacillus alginolyticus Strain XL-1

2000 ◽  
Vol 66 (9) ◽  
pp. 3945-3950 ◽  
Author(s):  
Harald J. Ruijssenaars ◽  
Sybe Hartmans ◽  
Jan C. Verdoes
Keyword(s):  
Signal Sequence ◽  
Fold Increase ◽  
Side Chain ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
E Coli ◽  
Mature Enzyme ◽  
Locust Bean ◽  
Encoding Gene ◽  
Amino Acid Signal

ABSTRACT Xanthan-modifying enzymes are powerful tools in studying structure-function relationships of this polysaccharide. One of these modifying enzymes is xanthan lyase, which removes the terminal side chain residue of xanthan. In this paper, the cloning and sequencing of the first xanthan lyase-encoding gene is described, i.e., thexalA gene, encoding pyruvated mannose-specific xanthan lyase of Paenibacillus alginolyticus XL-1. ThexalA gene encoded a 100,823-Da protein, including a 36-amino-acid signal sequence. The 96,887-Da mature enzyme could be expressed functionally in Escherichia coli. Like the native enzyme, the recombinant enzyme showed no activity on depyruvated xanthan. Compared to production by P. alginolyticus, a 30-fold increase in volumetric productivity of soluble xanthan lyase was achieved by heterologous production in E. coli. The recombinant xanthan lyase was used to produce modified xanthan, which showed a dramatic loss of the capacity to form gels with locust bean gum.

Cloning and sequencing of the gene encoding the cell surface glycoprotein of Haloarcula japonica strain TR-1

Extremophiles ◽  
1997 ◽  
Vol 1 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Hiromichi Wakai ◽  
Satoshi Nakamura ◽  
Hiroshi Kawasaki ◽  
Kei Takada ◽  
Shin Mizutani ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Glycoprotein ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Cell Surface Glycoprotein ◽  
Haloarcula Japonica
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