scholarly journals A Modified Fluorescence Polarization Immunoassay Method Incorporating Fat Emulsion(FE-FPIA) to Determine Cyclosporin A Concentrations in Rat Skin.

1999 ◽  
Vol 22 (5) ◽  
pp. 539-542
Author(s):  
Yuji KUROSAKI ◽  
Tomoya ASADA ◽  
Nanae YASUMOTO ◽  
Toshiaki NAKAMURA ◽  
Mikio MASADA ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay ◽  
Rat Skin ◽  
Fat Emulsion

Cross-reactivities of cyclosporin G (NVa2 cyclosporin) and metabolites in cyclosporin A immunoassays

1993 ◽  
Vol 39 (6) ◽  
pp. 1089-1092 ◽  
Author(s):  
R W Yatscoff ◽  
L J Langman ◽  
D F LeGatt
Keyword(s):  
Cyclosporin A ◽  
Enzyme Immunoassay ◽  
Fluorescence Polarization ◽  
Cross Reactivity ◽  
Fluorescence Polarization Immunoassay ◽  
Total Drug ◽  
Significant Variance ◽  
Routine Monitoring ◽  
The Cross

Abstract Immunoassays of cyclosporin A (CsA) have been routinely used to measure CsG. We investigated the cross-reactivities of CsG and its metabolites, as well as the proportion CsG constitutes in relation to total drug measured, for six CsG metabolites (GM1, GM9, GM4N, GM1c, GM1c9, GM19) in the following CsA assays: Sandimmune selective RIA (SS), Sandimmune nonselective RIA (NS), Cyclotrac SP-RIA (CT), fluorescence polarization immunoassay (FPIA), and enzyme immunoassay (EMIT). The cross-reactivity of CsG in these assays was as follows: SS, FPIA, CT, approximately 100%; NS, approximately 40%; EMIT, < 2%. The cross-reactivities of CsG metabolites were investigated in all assays except EMIT and varied among metabolites and assays. The most significant variance was found with the NS assay, where most of the metabolites exhibited cross-reactivities of > 40%. In contrast, in the SS, FPIA, and CT assays, cross-reactivities of < 5% were observed for most of the metabolites. The ranking of cross-reactivities of CsG metabolites in the assays is SS = CT < FPIA < NS. The degree of cross-reactivity did not change significantly when the SS, CT, and FPIA assays were calibrated with CsG instead of CsA--whether parent CsG was present or not. The data suggest that the SS, CT, and FPIA methods would be suitable for the routine monitoring of CsG.

scholarly journals Measurement of cyclosporin-A serum levels in the fluorescence polarization immunoassay and the clinical evaluation.

1988 ◽  
Vol 14 (1) ◽  
pp. 60-65
Author(s):  
YOUICHI HASEGAWA ◽  
HISAYOSHI SUGIHARA ◽  
AKIRA TAKAHASHI ◽  
HIROSHI HISHIDA ◽  
YASUTAKA SAKAMOTO ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Clinical Evaluation ◽  
Fluorescence Polarization ◽  
Serum Levels ◽  
Fluorescence Polarization Immunoassay

Comparison of cyclosporin G (Nva2-cyclosporin) concentrations measured in whole blood by monoclonal fluorescence polarization immunoassay, monoclonal radioimmunoassay, and HPLC

1993 ◽  
Vol 39 (6) ◽  
pp. 1050-1053 ◽  
Author(s):  
T M Annesley ◽  
R C Coombs ◽  
P J Orsulak
Keyword(s):  
Experimental Data ◽  
Renal Transplant ◽  
Cyclosporin A ◽  
Whole Blood ◽  
Fluorescence Polarization ◽  
Cross Reactivity ◽  
Fluorescence Polarization Immunoassay ◽  
Blood Concentrations ◽  
Transplant Patients ◽  
Liquid Chromatographic

Abstract Two commercial monoclonal immunoassays for monitoring cyclosporin A were used to measure whole-blood concentrations of the immunosuppressant cyclosporin G (CsG) in renal transplant patients. We performed a three-way comparison of these two immunoassays and HPLC. Although the two immunoassays agreed favorably, both the monoclonal fluorescence polarization immunoassay and the monoclonal RIA yielded higher CsG results for patients' specimens than did the liquid-chromatographic assay. The experimental data indicate that the observed differences are most likely due to the cross-reactivity of CsG metabolites in the immunoassays.

scholarly journals Comparison of fluorescence polarization immunoassay and radioimmunoassay of cyclosporin - A in human serum.

1988 ◽  
Vol 14 (6) ◽  
pp. 416-420
Author(s):  
KAZUTADA MATSUOKA ◽  
NAOYUKI SUEMATSU ◽  
ATSUSHI UENO ◽  
TATEO HORI ◽  
KOUICHIROU NOMATA ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Human Serum ◽  
Fluorescence Polarization ◽  
Fluorescence Polarization Immunoassay
Sign in / Sign up

Export Citation Format

Share Document