scholarly journals Internalization of Non-permeant Materials into Cultured Mammalian Cells by Vortex-Stirring in the Presence of a High Molecular Weight Polyacrylic Acid: Direct Evidence of Internalization and Its Dependence on the Cell Lines.

1997 ◽  
Vol 20 (7) ◽  
pp. 727-731 ◽  
Author(s):  
Naoaki SHIMIZU ◽  
Yutaka KAWAZOE ◽  
Tadahide FURUNO ◽  
Mamoru NAKANISHI
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Lines ◽  
Polyacrylic Acid ◽  
Mammalian Cells ◽  
Direct Evidence

scholarly journals MITOCHONDRIAL RNA FROM CULTURED ANIMAL CELLS

1970 ◽  
Vol 46 (2) ◽  
pp. 245-251 ◽  
Author(s):  
Bland S. Montenecourt ◽  
Margaret E. Langsam ◽  
Donald T. Dubin
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Lines ◽  
Ribosomal Rna ◽  
Gel Electrophoresis ◽  
Base Composition ◽  
Size Class ◽  
Mitochondrial Rna ◽  
Animal Cells ◽  
Molecular Weights

Discrete RNA fractions sedimenting slightly slower than 18s ribosomal RNA have been found in mitochondrial preparations from both hamster (BHK-21) and mouse (L-929) cells. This RNA could be separated into two components, present in approximately equimolar amounts, by prolonged zonal centrifugation or acrylamide gel electrophoresis. The hamster components had sedimentation constants averaging 16.8 and 13.4, and molecular weights (estimated by gel electrophoresis) averaging 0.74 and 0.42 x 106 daltons. Mixed labeling experiments showed that the mouse components sedimented and electrophoresed 3–6% more slowly than the corresponding hamster components. The RNA from both cell lines resembled mitochondrial ribosomal RNA from yeast and Neurospora in being GC poor, and in addition the larger and smaller components resembled each other in base composition. These results, taken with those of other recent studies, are compatible with the idea that our high molecular weight mitochondrial RNA is ribosomal; such RNA would then constitute a uniquely small size-class of ribosomal RNA.

A high molecular weight centrosomal protein of mammalian cells is antigenically related to myosin II

1992 ◽  
Vol 23 (2) ◽  
pp. 122-132 ◽  
Author(s):  
Eric Bailly ◽  
Nicoles Bordes ◽  
Michel Bornens ◽  
Catherine Klotz
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Mammalian Cells ◽  
Myosin Ii ◽  
Centrosomal Protein

scholarly journals Dymple, a Novel Dynamin-like High Molecular Weight GTPase Lacking a Proline-rich Carboxyl-terminal Domain in Mammalian Cells

1998 ◽  
Vol 273 (2) ◽  
pp. 1044-1051 ◽  
Author(s):  
Takahiro Kamimoto ◽  
Yasuo Nagai ◽  
Hiroshi Onogi ◽  
Yoshinao Muro ◽  
Takashi Wakabayashi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Mammalian Cells ◽  
Terminal Domain ◽  
Carboxyl Terminal Domain ◽  
Carboxyl Terminal

scholarly journals High-molecular-weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells

1983 ◽  
Vol 215 (3) ◽  
pp. 491-503 ◽  
Author(s):  
R A Childs ◽  
J Pennington ◽  
K Uemura ◽  
P Scudder ◽  
P N Goodfellow ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Lines ◽  
Galactose Oxidase ◽  
Embryonal Carcinoma Cell ◽  
Teratocarcinoma Cell ◽  
Differentiation Antigens ◽  
Carcinoma Cell Lines ◽  
Teratocarcinoma Cells ◽  
Mouse Teratocarcinoma

The carriers of the carbohydrate differentiation antigens I, i and SSEA-1 were investigated in embryonal carcinoma cell lines of mouse and differentiated cell lines derived from them. Glycoproteins were studied by immunostaining (‘Western blotting’) of total cell lysates and immunoprecipitation from lysates of galactose oxidase/NaB3H4-labelled cells; glycolipids were investigated by immunostaining of thin layer chromatograms. The antigenic activities detected by immunofluorescence of cell smears were reflected in the antigenicities of high-molecular-weight glycoproteins. These were polydisperse and markedly susceptible to digestion with endo-beta-galactosidase. Only the I antigen was detected on minor glycolipids. These observations indicate that glycoproteins rather than glycolipids are the major carriers of carbohydrate differentiation antigens I, i and SSEA-1 in the teratocarcinoma cell lines.

Sign in / Sign up

Export Citation Format

Share Document