scholarly journals High-molecular-weight glycoproteins are the major carriers of the carbohydrate differentiation antigens I, i and SSEA-1 of mouse teratocarcinoma cells

1983 ◽  
Vol 215 (3) ◽  
pp. 491-503 ◽  
Author(s):  
R A Childs ◽  
J Pennington ◽  
K Uemura ◽  
P Scudder ◽  
P N Goodfellow ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Lines ◽  
Galactose Oxidase ◽  
Embryonal Carcinoma Cell ◽  
Teratocarcinoma Cell ◽  
Differentiation Antigens ◽  
Carcinoma Cell Lines ◽  
Teratocarcinoma Cells ◽  
Mouse Teratocarcinoma

The carriers of the carbohydrate differentiation antigens I, i and SSEA-1 were investigated in embryonal carcinoma cell lines of mouse and differentiated cell lines derived from them. Glycoproteins were studied by immunostaining (‘Western blotting’) of total cell lysates and immunoprecipitation from lysates of galactose oxidase/NaB3H4-labelled cells; glycolipids were investigated by immunostaining of thin layer chromatograms. The antigenic activities detected by immunofluorescence of cell smears were reflected in the antigenicities of high-molecular-weight glycoproteins. These were polydisperse and markedly susceptible to digestion with endo-beta-galactosidase. Only the I antigen was detected on minor glycolipids. These observations indicate that glycoproteins rather than glycolipids are the major carriers of carbohydrate differentiation antigens I, i and SSEA-1 in the teratocarcinoma cell lines.

H-2 class I antigen expression on mouse teratocarcinoma cell lines

1985 ◽  
Vol 22 (6) ◽  
pp. 543-552 ◽  
Author(s):  
P. D�mant ◽  
M. Oudshoorn-Snoek
Keyword(s):  
Cell Lines ◽  
Antigen Expression ◽  
Class I ◽  
Teratocarcinoma Cell ◽  
Mouse Teratocarcinoma

scholarly journals Association of the H-Y male antigen with beta2-microglobulin on human lymphoid and differentiated mouse teratocarcinoma cell lines.

1978 ◽  
Vol 148 (1) ◽  
pp. 58-70 ◽  
Author(s):  
M Fellous ◽  
E Günther ◽  
R Kemler ◽  
J Wiels ◽  
R Berger ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Hla Antigens ◽  
Lymphoid Cell Line ◽  
Hla Antigen ◽  
Teratocarcinoma Cell ◽  
Daudi Cell ◽  
Human Male ◽  
Lymphoid Lines ◽  
Mouse Teratocarcinoma

The expression of the H-Y antigen has been tested on several human lymphoid lines and mouse teratocarcinoma cell lines during differentiation. The human male lymphoid cell line Raji is a very useful target for studies of the H-Y antigen by lymphocytotoxicity test with rat anti-H-Y sera. With a few exceptions, all cells carrying the Y chromosome were H-Y positive. One of the exceptions is the human Daudi cell line which, besides lacking H-Y antigen, also lacks beta2-microglobulin. We have studied a possible association between the H-Y antigen, beta2-microglobulin, and HLA antigen with redistribution experiments. The results strongly suggest that H-Y antigen is not associated with HLA antigens but with beta2-microglobulin.

Embryonal Carcinoma Cell Lines Stably Transfected with mRARβ2-lacZ: Sensitive System for Measuring Levels of Active Retinoids

1999 ◽  
Vol 250 (2) ◽  
pp. 284-297 ◽  
Author(s):  
Edwin Sonneveld ◽  
Christina E. van den Brink ◽  
Bas-jan M. van der Leede ◽  
Malcolm Maden ◽  
Paul T. van der Saag
Keyword(s):  
Cell Lines ◽  
Embryonal Carcinoma ◽  
Carcinoma Cell ◽  
Embryonal Carcinoma Cell ◽  
Carcinoma Cell Lines

scholarly journals Three mouse monoclonal antibodies to human differentiation antigens: reactivity with two mucin-like antigens and with connective tissue fibers.

1985 ◽  
Vol 33 (11) ◽  
pp. 1095-1102 ◽  
Author(s):  
M J Mattes ◽  
K Look ◽  
J L Lewis ◽  
L J Old ◽  
K O Lloyd
Keyword(s):  
Monoclonal Antibodies ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Cell Lines ◽  
Frozen Sections ◽  
Cultured Fibroblasts ◽  
Differentiation Antigens ◽  
Normal Tissues ◽  
Carcinoma Cell Lines ◽  
Histologic Types

Three human differentiation antigens (MU78, MT334, and MQ49) have been defined by mouse monoclonal antibodies developed from mice immunized with ovarian carcinoma cell lines. Their distribution was determined on 148 cultured cell lines of various histologic types and on frozen sections of 16 normal tissues. MU78 was found in fibrillar structures in soft connective tissue with a distribution resembling that of elastin fibers; however, elastin fibers in elastic cartilage and in the aorta were nonreactive. MU78 was detected in cultured carcinoma cells of various histologic types, where it had a nonfibrillar, cytoplasmic distribution, but was not detected in normal epithelial cells in frozen sections. Cultured fibroblasts, astrocytomas, melanomas, and lymphomas did not contain MU78. In cell lines, MU78 appears to be a protein of 2000-5000 daltons. The other two antigens, MT334 and MQ49, are both mucin-like molecules, and the determinants are probably carbohydrate in nature. Of the normal tissues examined, MT334 was detected only in goblet cells of the colon, though it was present in a variety of carcinomas in culture. It was detected as both a cytoplasmic and secreted component. MQ49 was detected in various secretory epithelial cells, in Hassall's corpuscles in the thymus, and in cultured carcinomas of various histologic types. It was found on the cell surface as well as in the cytoplasm and is present on a glycolipid as well as on a sulfated mucin. These results, and results of other recent studies, demonstrate the importance of mucin-like molecules as antigens in epithelial cells and secretions.

scholarly journals MITOCHONDRIAL RNA FROM CULTURED ANIMAL CELLS

1970 ◽  
Vol 46 (2) ◽  
pp. 245-251 ◽  
Author(s):  
Bland S. Montenecourt ◽  
Margaret E. Langsam ◽  
Donald T. Dubin
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Lines ◽  
Ribosomal Rna ◽  
Gel Electrophoresis ◽  
Base Composition ◽  
Size Class ◽  
Mitochondrial Rna ◽  
Animal Cells ◽  
Molecular Weights

Discrete RNA fractions sedimenting slightly slower than 18s ribosomal RNA have been found in mitochondrial preparations from both hamster (BHK-21) and mouse (L-929) cells. This RNA could be separated into two components, present in approximately equimolar amounts, by prolonged zonal centrifugation or acrylamide gel electrophoresis. The hamster components had sedimentation constants averaging 16.8 and 13.4, and molecular weights (estimated by gel electrophoresis) averaging 0.74 and 0.42 x 106 daltons. Mixed labeling experiments showed that the mouse components sedimented and electrophoresed 3–6% more slowly than the corresponding hamster components. The RNA from both cell lines resembled mitochondrial ribosomal RNA from yeast and Neurospora in being GC poor, and in addition the larger and smaller components resembled each other in base composition. These results, taken with those of other recent studies, are compatible with the idea that our high molecular weight mitochondrial RNA is ribosomal; such RNA would then constitute a uniquely small size-class of ribosomal RNA.

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