scholarly journals Urinary Protein Fractions in Healthy Subjects Using Cellulose Acetate Membrane Electrophoresis Followed by Staining with Acid Violet 17.

1994 ◽  
Vol 17 (10) ◽  
pp. 1355-1357 ◽  
Author(s):  
Nobuo HIRATSUKA ◽  
Kiyoko SHIBA ◽  
Katsumi SHINOMURA ◽  
Seijin HOSAKI ◽  
Hiroko CHO ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Healthy Subjects ◽  
Urinary Protein ◽  
Protein Fractions ◽  
Cellulose Acetate Membrane ◽  
Acid Violet

scholarly journals Prediction of renal damages using urinary protein profiles in cellulose acetate membrane electrophoresis

2015 ◽  
Vol 59 (1) ◽  
pp. 51-53
Author(s):  
Aki Nakayama ◽  
Minoru Sakatsume ◽  
Kiyoko Shiba ◽  
Shiro Iijima
Keyword(s):  
Cellulose Acetate ◽  
Urinary Protein ◽  
Cellulose Acetate Membrane ◽  
Protein Profiles

Evaluation of a new cellulose acetate membrane for electrophoresis to analyze protein fractions by JOKO-CTE8000.

2007 ◽  
Vol 51 (2) ◽  
pp. 113-117
Author(s):  
Kyoko Miyazaki ◽  
Masayoshi Yoneyama ◽  
Miho Takahasi ◽  
Teruo Ekami ◽  
Hiroaki Oonisi ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Protein Fractions ◽  
Cellulose Acetate Membrane

scholarly journals Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden

1995 ◽  
Vol 37 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Grace Mary Leal-Bacelar ◽  
Hermínia Y. Kanamura ◽  
Sumie Hoshino-Shimizu ◽  
Dirce Mary C. Lima ◽  
Clarice P. Abrantes-Lemos ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Cellulose Acetate ◽  
Healthy Subjects ◽  
Worm Burden ◽  
Retrospective Studies ◽  
Cellulose Acetate Membrane ◽  
Serum Samples ◽  
Diffusion Assay

An immunoprecipitation technique, ELIEDA (enzyme-linked-immuno-electro-diffusion assay), was evaluated for the diagnosis of Schistosoma mansoni infection with low worm burden. One hundred of serum samples from patients excreting less than 600 eggs per gram of feces (epg), with unrelated diseases and clinically healthy subjects were studied. In patients with egg counts higher than 200 epg, the sensitivities of IgM and IgG ELIEDA were 1.000 and 0.923, respectively, not differing from other Serologic techniques, such as indirect hemaglutination (IHAT), immunofluorescence (IFT) tests and immuno-electrodiffusion assay (IEDA). However in patients with low egg counts (< 100 epg), the IgG ELIEDA provided better results (0.821) than IgM ELIEDA (0.679), showing sensitivity that did not differ from that of IgG IFT (0.929), but lower than that of IgM IFT (0.964). However, its sensivity was higher than that found with IHAT (0.607) and IEDA (0.536). The specificity of IgG ELIEDA was comparable to that of other techniques. The data indicate that IgG ELIEDA might be useful for the diagnosis of slight S. mansoni infections, and the cellulose acetate membrane strips can be stored for further retrospective studies.

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