scholarly journals Adriamycin-Induced Lipid Peroxidation of Erythrocyte Membranes in the Presence of Ferritin and the Inhibitory Effect of Ceruloplasmin.

1993 ◽  
Vol 16 (7) ◽  
pp. 664-667 ◽  
Author(s):  
Toshiaki MIURA ◽  
Sanae MURAOKA ◽  
Taketo OGISO
Keyword(s):  
Lipid Peroxidation ◽  
Inhibitory Effect ◽  
Erythrocyte Membranes

New Galantamine Derivatives with Inhibitory Effect on Acetylcholinesterase Activity

2021 ◽  
Vol 83 (3) ◽  
pp. 1211-1220 ◽  
Author(s):  
Maria I. Lazarova ◽  
Daniela S. Tsekova ◽  
Lyubka P. Tancheva ◽  
Kiril T. Kirilov ◽  
Diamara N. Uzunova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Memory Performance ◽  
Acetylcholinesterase Activity ◽  
Inhibitory Effect ◽  
Long Term Memory ◽  
Molegro Virtual Docker ◽  
Ache Inhibitors ◽  
Hybrid Molecules ◽  
Ache Inhibitory Activity ◽  
Biological Assessments

Background: Inhibitors of acetylcholinesterase (AChE) are used to treat many disorders, among which are neurodegenerative upsets, like Alzheimer’s disease (AD). One of the limited licensed AChE inhibitors (AChEIs) used as drugs is the natural compound galantamine (Gal). Objective: As Gal is a toxic compound, here we expose data about its four derivatives in hybrid peptide-norgalantamine molecules, which have shown 100 times lower toxicity. Methods: Four newly synthesized galantamine derivatives have been involved in docking analysis made by Molegro Virtual Docker. Biological assessments were performed on ICR male mice. The change in short and long-term memory performance was evaluated by passive avoidance test. AChE activity and levels of main oxidative stress parameters: lipid peroxidation, total glutathione (GSH), enzyme activities of catalase (CAT), superoxide dismutase, and glutathione peroxidase were measured in brain homogenates. Results: Our experimental data revealed that the new hybrid molecules did not impair memory performance in healthy mice. Two of the compounds demonstrated better than Gal AChE inhibitory activity in the brain. None of them changed the level of lipid peroxidation products, one of the compounds increased GSH levels, and all of them increased CAT enzyme activity. Conclusion: The new galantamine-peptide hybrids demonstrated a potential for inhibition of AChE and antioxidant activity and deserve further attention.

scholarly journals Elongation of the Cytoplasmic Tail Interferes with the Fusion Activity of Influenza Virus Hemagglutinin

1998 ◽  
Vol 72 (5) ◽  
pp. 3554-3559 ◽  
Author(s):  
Masanobu Ohuchi ◽  
Christian Fischer ◽  
Reiko Ohuchi ◽  
Astrid Herwig ◽  
Hans-Dieter Klenk
Keyword(s):  
Intracellular Transport ◽  
Critical Role ◽  
Simian Virus 40 ◽  
Cytoplasmic Tail ◽  
Surface Expression ◽  
Inhibitory Effect ◽  
Simian Virus ◽  
Cell Surface Expression ◽  
Erythrocyte Membranes ◽  
Fusion Pores

ABSTRACT The hemagglutinin (HA) of fowl plague virus was lengthened and shortened by site-specific mutagenesis at the cytoplasmic tail, and the effects of these modifications on HA functions were analyzed after expression from a simian virus 40 vector. Elongation of the tail by the addition of one to six histidine (His) residues did not interfere with intracellular transport, glycosylation, proteolytic cleavage, acylation, cell surface expression, and hemadsorption. However, the ability to induce syncytia at a low pH decreased dramatically depending on the number of His residues added. Partial fusion (hemifusion), assayed by fluorescence transfer from octadecylrhodamine-labeled erythrocyte membranes, was also reduced, but even with the mutant carrying six His residues, significant transfer was observed. However, when the formation of fusion pores was examined with hydrophilic fluorescent calcein, transfer from erythrocytes to HA-expressing cells was not observed with the mutant carrying six histidine residues. The addition of different amino acids to the cytoplasmic tail of HA caused an inhibitory effect similar to that caused by the addition of His. On the other hand, a mutant lacking the cytoplasmic tail was still able to fuse at a reduced level. These results demonstrate that elongation of the cytoplasmic tail interferes with the formation and enlargement of fusion pores. Thus, the length of the cytoplasmic tail plays a critical role in the fusion process.

Studies on the Antioxidant Activities of Total Alkaloids from the Fruits of Evodia rutaecarpa (Juss.) Benth

2011 ◽  
Vol 396-398 ◽  
pp. 52-55 ◽  
Author(s):  
Ming Xiong Tan ◽  
Yan Cheng Liu ◽  
Xu Jian Luo ◽  
Dong Qing Li
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Inhibitory Effect ◽  
Dpph Radical ◽  
Synthetic Antioxidant ◽  
Total Alkaloids ◽  
Evodia Rutaecarpa ◽  
Total Antioxidant

The total alkaloids (TAE) were obtained from the fruits of Evodia rutaecarpa (Juss.) Benth. The antioxidant activities of TAE towards the inhibitory effect on 2, 2’-diphenyl-1-picrylhydrazyl (DPPH) free radical, total antioxidant capacity, and inhibition on lipid peroxidation were investigated. TAE was found that the total antioxidant capacity and inhibitory lipid peroxidation are superior to synthetic antioxidant 2, 6-di-ter-butyl-4-methylphenol (BHT), but scavenging activity on DPPH radical is lower than that of BHT at the same condition.

Antioxidant Activity of Flavones from Scutellaria baicalensis in Lecithin Liposomes

1997 ◽  
Vol 52 (11-12) ◽  
pp. 817-823 ◽  
Author(s):  
Janina Gabrielska ◽  
Jan Oszmiańsk ◽  
Romuald Żyłka ◽  
Małgorzata Komorowska
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Skin Diseases ◽  
Inhibitory Effect ◽  
Scutellaria Baicalensis ◽  
Butylated Hydroxytoluene ◽  
Protective Effects ◽  
Liposome Membrane ◽  
Beneficial Effects ◽  
Phosphatidylcholine Liposome

Abstract Trihydroxyflavones of Scutellaria baicalensis, Antioxidant Activity, Liposome, Peroxidation, MDA The antioxidant effect of a trihydroxyflavone extract from Scutellaria baicalensis on oxida­ tion induced by ultraviolet light, was studied with phosphatidylcholine liposome membrane. Also, as standards, the antioxidative activity of baicalin, wogonin, baicalein and butylated hydroxytoluene (BHT) was investigated. Comparison of the protective effects of thecom­pounds studied against photoinduced lipid peroxidation in lecithin liposome membranes showed that: (1) the inhibitory effect of those compounds (at 1.2 mol% antioxidant content in liposomes) on TBA reactive materials from lipid peroxidation decreased in the order of baicalin > BHT ≅ Scutellaria baicalensis. These were found much greater than wogonin and baicalein; (2) the depressed effect of those compounds (at 1.1 mol% compounds content in liposomes) on the production of conjugated dienes (proportional to oxidation index) could be classified as follows: Scutellaria baicalensis ≅ baicalin > BHT, these three were found more active much greather than baicalein and wogonin. Results obtained by ESR measure­ment confirm that Scutellaria baicalensis extract and the BHT compound significantly de­ pressed the effect of liposome oxidation. It was found that the new trihydroxyflavones of Scutellaria baicalensis, ensured a very satisfactory concentration-dependent protection of the liposome membrane against UV-induced oxidation. These findings suggest that some of the beneficial effects of the extract of the Scutellaria baicalensis can be mediated in certain diseases (for example in skin diseases) by their ability to scavenge free radicals and by their protective effect on lipid peroxidation caused by sunlight irradiation.

scholarly journals Comparison of Antioxidants: The Limited Correlation between Various Assays of Antioxidant Activity

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3244
Author(s):  
Katarzyna Naparło ◽  
Mirosław Soszyński ◽  
Grzegorz Bartosz ◽  
Izabela Sadowska-Bartosz
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Experimental System ◽  
Natural Antioxidants ◽  
Egg Yolk ◽  
Erythrocyte Membranes ◽  
Oxygen Species ◽  
Inhibitory Effects

The inhibitory effects a range of synthetic and natural antioxidants on lipid peroxidation of egg yolk and erythrocyte membranes induced by a free radical generator 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) was compared, with significant differences being found between both systems. When the protection by selected antioxidants against the effects of AAPH on erythrocytes (hemolysis, oxidation of hemoglobin and glutathione (GSH) and generation of reactive oxygen species (ROS)) was studied, most antioxidants were protective, but in some tests (oxidation of hemoglobin and GSH) some acted as prooxidants, inducing oxidation in the absence of AAPH and enhancing the AAPH-induced oxidation. These results demonstrate a diversified action of antioxidants in different systems and point to a need for careful extrapolation of any conclusions drawn from one parameter or experimental system to another.

scholarly journals Synthesis and biological activity of N-substituted-tetrahydro-γ-carbolines containing peptide residues

2014 ◽  
Vol 10 ◽  
pp. 155-162 ◽  
Author(s):  
Nadezhda V Sokolova ◽  
Valentine G Nenajdenko ◽  
Vladimir B Sokolov ◽  
Daria V Vinogradova ◽  
Elena F Shevtsova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Biological Activity ◽  
Permeability Transition ◽  
Liver Mitochondria ◽  
Inhibitory Effect ◽  
Rat Liver Mitochondria ◽  
Terminal Alkyne ◽  
Peptide Fragments ◽  
Prooxidant Activity ◽  
Peptide Conjugates

The synthesis of novel peptide conjugates of N-substituted-tetrahydro-γ-carbolines has been performed using the sequence of the Ugi multicomponent reaction and Cu(I)-catalyzed click chemistry. The effect of obtained γ-carboline–peptide conjugates on the rat liver mitochondria was evaluated. It was found that all compounds in the concentration of 30 µM did onot induce depolarization of mitochondria but possessed some inhibitory effect on the mitochondria permeability transition. The original N-substituted-tetrahydro-γ-carbolines containing an terminal alkyne group demonstrated a high prooxidant activity, whereas their conjugates with peptide fragments slightly inhibited both autooxidation and the t-BHP-induced lipid peroxidation.

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