scholarly journals Comparison of Antioxidants: The Limited Correlation between Various Assays of Antioxidant Activity

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3244
Author(s):  
Katarzyna Naparło ◽  
Mirosław Soszyński ◽  
Grzegorz Bartosz ◽  
Izabela Sadowska-Bartosz
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Experimental System ◽  
Natural Antioxidants ◽  
Egg Yolk ◽  
Erythrocyte Membranes ◽  
Oxygen Species ◽  
Inhibitory Effects

The inhibitory effects a range of synthetic and natural antioxidants on lipid peroxidation of egg yolk and erythrocyte membranes induced by a free radical generator 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) was compared, with significant differences being found between both systems. When the protection by selected antioxidants against the effects of AAPH on erythrocytes (hemolysis, oxidation of hemoglobin and glutathione (GSH) and generation of reactive oxygen species (ROS)) was studied, most antioxidants were protective, but in some tests (oxidation of hemoglobin and GSH) some acted as prooxidants, inducing oxidation in the absence of AAPH and enhancing the AAPH-induced oxidation. These results demonstrate a diversified action of antioxidants in different systems and point to a need for careful extrapolation of any conclusions drawn from one parameter or experimental system to another.

scholarly journals A REVIEW ON ANTIOXIDANT METHODS

2016 ◽  
pp. 14 ◽  
Author(s):  
Dontha Sunitha
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Oxygen Species ◽  
Antioxidant Assay ◽  
Antioxidant Effects ◽  
In Vitro Antioxidant Activity

<p>ABSTRACT<br />To provide an outlook of the various available methods of antioxidant activity. Various available in vitro and in vivo methods are listed and the<br />procedure to perform the method, its mechanism is also explained in brief. 1,1-diphenyl-2-picrylhydrazyl method was found to be used mostly for the<br />in vitro antioxidant activity evaluation purpose while lipid peroxidation was found as mostly used in vivo antioxidant assay. An ethanol was with the<br />highest frequency as a solvent for extraction purpose. Summarized information on the various methods available provides with reliable information<br />to confirm the benefits of antioxidant effects.<br />Keywords: Antioxidant activity, Reactive oxygen species, Free radical, 1,1-diphenyl-2-picrylhydrazyl, Flavonoid.</p>

scholarly journals Effect of bromfenac on free radical oxidation in model systems

2019 ◽  
Vol 100 (4) ◽  
pp. 636-641 ◽  
Author(s):  
E F Galimova ◽  
Z G Khaibullina ◽  
D A Enikeev ◽  
Yu L Bortsova ◽  
K S Mochalov ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Reactive Oxygen ◽  
Free Radical Oxidation ◽  
Model Systems ◽  
Oxygen Species ◽  
Radical Oxidation ◽  
Anti Inflammatory

Aim. The study of free radical oxidation processes in an experiment on model systems using the anti-inflammatory drug bromfenac (nakwan) widely used in ophthalmology for the treatment of infectious and inflammatory diseases of the anterior chamber of the eye. Methods. The antioxidant capacity of the drug was evaluated by chemiluminescence registration and analysis of model systems that generate reactive oxygen species and reproduce lipid peroxidation processes using the chemiluminomer CL-003. The following parameters of spontaneous and induced chemiluminescence were determined: light sum and maximum luminescence amplitude, duration of latent period, amplitude of fast and slow flash. Results. When tested in vitro in two different model systems, a high antioxidant activity of the studied drug was established, up to complete suppression of chemiluminescence when 90 μg of bromfenac was added to the incubation medium, which characterizes the inhibition of the generation of reactive oxygen species. A significant increase in total antioxidant activity with bromfenac was also demonstrated, which is reflected by the integral parameter of chemiluminescence — light sum which decreased with the introduction of 10 μg of the drug by 1.2 times, and with 90 μg by 1.5 times. A comparative analysis of the antioxidant properties of various nonsteroidal anti-inflammatory drugs used in ophthalmic practice demonstrated a more pronounced efficacy of bromfenac compared to ketorolac, the use of which was not accompanied by statistically significant changes in chemiluminescence. A very important mechanism of the positive effect of bromfenac is the direct dependence of the action on its quantity in the reaction medium, which opens up prospects for the controlled correction of free radical phenomena and the excessive activation of lipid peroxidation in the imbalance of the pro- and antioxidant processes in biological systems. Conclusion. It is suggested that the protective effects of the drug in various infectious-inflammatory lesions of the eye can be determined, along with previously known properties, its antioxidant activity, restriction of increased production of reactive oxygen species and oxidative stress phenomena.

Chemistry and antioxidant activity of plants containing some phenolic compounds

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Reactive Oxygen Species ◽  
Health Promotion ◽  
Antioxidant Activity ◽  
Natural Products ◽  
Natural Antioxidants ◽  
Health Impact ◽  
Oxygen Species ◽  
Living Things ◽  
Effective Antioxidant ◽  
Great Health

Natural antioxidants inhibit the adverse effects of the reactive oxygen species produced in living things and enable to survive. Depending on this principle, humans started using some plant products to cure from various diseases. Natural products have proved of great health impact on human traditionally and scientifically. Moreover, the research has so far done in the field of natural antioxidants. Therefore, antioxidants have been traditionally used as well as experimentally proved beneficial. The antioxidant components of the natural products constitute the major source of human health promotion and maintenance. The nature is still the perfect source for health promotion and for the supplementation of safe drugs. Great attention is mandatory to explore many underestimated plants with highly effective antioxidant activity. Few classes of natural products have received as much attention as phenolics and polyphenols.

scholarly journals The influence of light exposure on free radical processes in the lungs of rats with pleurisy

2016 ◽  
Author(s):  
O. M. Larycheva
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Free Radical ◽  
Light Exposure ◽  
Reactive Oxygen ◽  
Male Rats ◽  
Oxygen Species ◽  
Free Radical Processes ◽  
Radical Processes

The experiments on male rats demonstrated that under the conditions of pleurisy induced by carrageenan and short melatonin deficit, a significant increase in the level of reactive oxygen species and products of lipid peroxidation in the lungs takes place.

scholarly journals Plastoquinone: possible involvement in plant disease resistance.

2002 ◽  
Vol 49 (3) ◽  
pp. 775-780 ◽  
Author(s):  
Urszula Maciejewska ◽  
Lidia Polkowska-Kowalczyk ◽  
Ewa Swiezewska ◽  
Anna Szkopinska
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Disease Resistance ◽  
Plant Disease Resistance ◽  
Plant Disease ◽  
Solanum Nigrum ◽  
Ros Production ◽  
Oxygen Species

The plant Solanum nigrum treated with the pathogen Phytophthora infestans-derived elicitor responded by elevated reactive oxygen species (ROS) production, lipid peroxidation and lipoxygenase (EC 1.13.11.12) activity in comparison with control plants indicating that oxidative stress took place. We demonstrate that these events are accompanied by a significant increase in plastoquinone (PQ) level. It is postulated that PQ may be associated with mechanisms maintaining a tightly controlled balance between the accumulation of ROS and antioxidant activity that determines the full expression of effective defence.

scholarly journals Role of Natural Antioxidants on Neuroprotection and Neuroinflammation

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 608
Author(s):  
Domenico Nuzzo
Keyword(s):  
Reactive Oxygen Species ◽  
Energy Metabolism ◽  
Respiratory Chain ◽  
Reactive Oxygen ◽  
Natural Antioxidants ◽  
Oxygen Species

All cells continuously generate reactive oxygen species (ROS) through the respiratory chain during the energy metabolism process [...]

scholarly journals The Effects of Bioactive Compounds from Blueberry and Blackcurrant Powder on Oat Bran Pastes: Enhancing In Vitro Antioxidant Activity and Reducing Reactive Oxygen Species in Lipopolysaccharide-Stimulated Raw264.7 Macrophages

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 388
Author(s):  
Xiao Dan Hui ◽  
Gang Wu ◽  
Duo Han ◽  
Xi Gong ◽  
Xi Yang Wu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Raw264.7 Macrophages ◽  
Oat Bran

In this study, blueberry and blackcurrant powder were chosen as the phenolic-rich enrichments for oat bran. A Rapid Visco Analyser was used to form blueberry and blackcurrant enriched oat pastes. An in vitro digestion process evaluated the changes of phenolic compounds and the in vitro antioxidant potential of extracts of pastes. The anthocyanidin profiles in the extracts were characterised by the pH differential method. The results showed that blueberry and blackcurrant powder significantly increased the content of phenolic compounds and the in vitro antioxidant capacity of pastes, while the total flavonoid content decreased after digestion compared to the undigested samples. Strong correlations between these bioactive compounds and antioxidant values were observed. Lipopolysaccharide-stimulated RAW264.7 macrophages were used to investigate the intracellular antioxidant activity of the extracts from the digested oat bran paste with 25% enrichment of blueberry or blackcurrant powder. The results indicated that the extracts of digested pastes prevented the macrophages from experiencing lipopolysaccharide (LPS)-stimulated intracellular reactive oxygen species accumulation, mainly by the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor erythroid 2-related factor 2 (Nrf2) signalling pathway. These findings suggest that the bioactive ingredients from blueberry and blackcurrant powder enhanced the in vitro and intracellular antioxidant capacity of oat bran pastes, and these enriched pastes have the potential to be utilised in the development of the functional foods.

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