scholarly journals Apoptotic Cell Death of High Polyploid Cells in a Cultured Sarcoma Cell Line.

1998 ◽  
Vol 23 (4) ◽  
pp. 231-237 ◽  
Author(s):  
Zhi-Ping Zong ◽  
Kohzaburo Fujikawa-Yamamoto ◽  
Takahide Ota ◽  
Manabu Murakami ◽  
Ai-Li Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Sarcoma Cell ◽  
Sarcoma Cell Line ◽  
High Polyploid ◽  
Polyploid Cells

Therapeutic Efficacy of FTY720 In a Rat Model of LGL Leukemia

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 776-776
Author(s):  
Xin Liu ◽  
Aijun Liao ◽  
Hong-Gang Wang ◽  
Dhimant Desai ◽  
Shantu Amin ◽  
...  
Keyword(s):  
Cell Death ◽  
Nk Cells ◽  
Cell Line ◽  
Rat Model ◽  
Therapeutic Efficacy ◽  
Apoptotic Cell ◽  
Treated Group ◽  
Apoptotic Cell Death ◽  
Leukemia Cell Line ◽  
Lgl Leukemia

Abstract Abstract 776 Natural kill cell large granular lymphocytic (NK-LGL) leukemia is a fatal disorder with death occurring in days to weeks following diagnosis. There is no known curative therapy for this disease. Therefore, there is an urgent unmet need for development of new therapeutics for this deadly leukemia. Fischer F344 rat LGL leukemia model has been established as an important experimental model for the study of NK-LGL leukemia progression and closely resembles human aggressive NK-LGL leukemia exhibiting clonal expansion of CD3-CD8a+ lymphocytes. FTY720, a new immunosuppressant, has been studied for its putative anti-cancer properties in the recent years. At four weeks after transplantation of leukemic LGL cell line, the rats displayed early signs of leukemia, including weight loss, rough hair coat and increased level of neutrophils. By week 5, circulating blasts, anemia, thrombocytopenia, and splenomegaly were observed. These leukemic rats were then injected intraperitoneally with 4.5mg/kg of FTY720 or PBS every day over a 4-week treatment period. Animals died within the next 1 to 2 weeks if treated with PBS. The median survival in PBS treated group was 41 days compared to 51 days in FTY720 treated group (Mantel-Cox test, p<0.0001). Importantly, 5 of 16 leukemic rats treated with FTY720 had maintenance of normal blood counts without circulating blasts suggesting achievement of complete clinical remission. The remaining eleven leukemic rats treated with FTY720 had a transient improvement as evidenced by reduction of white blood cell counts and elevated platelet counts after two weeks treatment. Subsequently, however, blast counts rose and animals died within the following one week. To further determine remission status, the five responsive animals were euthanized after cessation of the treatment. At necropsy, we found these rats had normal levels of CD3-CD8a+ LGL cells in the blood, marrow, and spleen. Of note, the eleven leukemic rats not achieving remission also displayed significant reduction of LGL cells in these tissues, to lesser extent. Examination of spleen sections from rats responsive to FTY720 showed normal splenic histology. In contrast, leukemic rats not achieving remission showed leukemic LGL infiltration of the red pulp and depletion of the white pulp. Interestingly, pro-apoptotic proteins Bax and Bak were dramatically increased while anti-apoptotic protein Mcl-1 was decreased in the spleens of the rats achieving remission; however, they remained unchanged in leukemic rats not achieving remission. These data indicate that in vivo therapeutic efficacy of FTY720 may be a consequence of modulation of anti-apoptosis signaling which led to resolution of leukemic cell infiltration. We then extended these studies to NK LGL from patients. Initial experiments demonstrated that FTY720 displayed dose- and time-dependent apoptotic cell death in PBMC from NK-LGL leukemia patients (CD3−CD56+>80%). In contrast, treatment with 10uM FTY720 did not induce significant cytotoxic effects in PBMC from normal donors, or normal NK cells. In addition, treatment of human and rat NK-LGL leukemia cells with FTY720 led to caspase-dependent apoptosis, generation of reactive oxygen species (ROS), and Mcl-1 degradation which did not occur at the transcriptional level. Of interest, inhibition of ROS rescued FTY720 induced apoptosis in leukemic NK cells. Moreover, efficient knockdown of Mcl-1 resulted in more than two fold increase in apoptotic cell death of NKL, a human NK-LGL leukemia cell line. Collectively, these results indicate efficacy of FTY720 in a rat model of NK LGL leukemia via production of ROS and decreased mcl-1 expression or signaling. Disclosures: No relevant conflicts of interest to declare.

N-Nitrosodimethylamine-Mediated Cytotoxicity in a Cell Line Expressing P450 2E1: Evidence for Apoptotic Cell Death

1999 ◽  
Vol 157 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Hsia-lien Lin ◽  
Leslie A. Parsels ◽  
Jonathan Maybaum ◽  
Paul F. Hollenberg
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
A Cell

Apoptotic Cell Death of a Hybrid Motoneuron Cell Line Induced by Immunoglobulins from Patients with Amyotrophic Lateral Sclerosis

2002 ◽  
Vol 63 (6) ◽  
pp. 2365-2368 ◽  
Author(s):  
Maria E. Alexianu ◽  
A. Habib Mohamed ◽  
R. Glenn Smith ◽  
Luis V. Colom ◽  
Stanley H. Appel
Keyword(s):  
Cell Death ◽  
Amyotrophic Lateral Sclerosis ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Motoneuron Cell ◽  
Lateral Sclerosis

Cytokine-induced apoptotic cell death in a mouse pancreatic beta-cell line: inhibition by Bcl-2

Diabetologia ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 530-536 ◽  
Author(s):  
H. Iwahashi ◽  
T. Hanafusa ◽  
Y. Eguchi ◽  
H. Nakajima ◽  
J. Miyagawa ◽  
...  
Keyword(s):  
Cell Death ◽  
Beta Cell ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Pancreatic Beta Cell ◽  
Apoptotic Cell Death ◽  
Beta Cell Line

scholarly journals Origin of Microcells in the Human Sarcoma Cell Line HT-1080

1999 ◽  
Vol 18 (2) ◽  
pp. 73-85 ◽  
Author(s):  
Indulis Buiķis ◽  
Līga Harju ◽  
Tālivaldis Freivalds
Keyword(s):  
Cell Line ◽  
Apoptotic Cell ◽  
Sarcoma Cell ◽  
Electron Microscopic ◽  
Sarcoma Cell Line ◽  
Lung Resistance Protein ◽  
Coiled Bodies ◽  
Lung Resistance ◽  
Resistance Protein ◽  
Human Sarcoma

The aim of this study was to investigate the development of microcells in the human sarcoma cell line HT‐1080 after interference with thiophosphamidum. We found that damaged interphase macrocells located at the projection of the nucleolus may form one or several microcells. The micronuclei of the microcells intensively incorporate the thymidine analogue 5‐bromo‐2'‐deoxyuridine and strongly express argyrophilic nucleolar organiser region proteins. At an early phase of the development, the micronuclei contain fragmented DNA, but in subsequent phases, the micronuclei accumulate polymeric DNA, simultaneously with an increase in their size. After desintegration of the damaged macrocell, the microcells appear in the intercellular space. The microcells can enter mitosis and they strongly express the lung resistance protein. Electron microscopic observations suggest that coiled bodies are involved in the development of the microcells. Since the observed path of microcell formation differs from apoptotic cell fragmentation into apoptotic bodies, we propose a new term for this microcell development:sporosis. We suggest that self‐renewal of the tumour stem cells is likely based on sporosis.

Characteristics of etoposide-induced apoptotic cell death in the U-937 human lymphoma cell line

2001 ◽  
Vol 12 (8) ◽  
pp. 699-705 ◽  
Author(s):  
Petra Martinsson ◽  
Gunnar Liminga ◽  
Peter Nygren ◽  
Rolf Larsson
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Lymphoma Cell ◽  
Lymphoma Cell Line ◽  
Human Lymphoma

Cytotoxic potential of dispirooxindolo/acenaphthoquino andrographolide derivatives against MCF-7 cell line

MedChemComm ◽  
2015 ◽  
Vol 6 (4) ◽  
pp. 702-707 ◽  
Author(s):  
Debanjana Chakraborty ◽  
Arindam Maity ◽  
Chetan K. Jain ◽  
Abhijit Hazra ◽  
Yogesh P. Bharitkar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Cell Line ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Cytotoxic Potential ◽  
Cell Death Pathway ◽  
Line P ◽  
Mcf 7

Dispiro andrographolides induce a caspase-dependent apoptotic cell death pathway in breast cancer (MCF-7) cells.

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