scholarly journals Quantification of Cell Nuclei Isolated from Hepatocytes by Cell Lysis with Nonionic Detergent in Citric Acid.

1991 ◽  
Vol 16 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Yoshifumi Horiuti ◽  
Motohiro Ogishima ◽  
Kazuo Yano ◽  
Yuzo Shibuya
Keyword(s):  
Citric Acid ◽  
Cell Lysis ◽  
Cell Nuclei ◽  
Nonionic Detergent

scholarly journals THE ISOLATION OF CELL NUCLEI IN NON-AQUEOUS MEDIA

1952 ◽  
Vol 35 (3) ◽  
pp. 529-557 ◽  
Author(s):  
V. Allfrey ◽  
H. Stern ◽  
A. E. Mirsky ◽  
H. Saetren
Keyword(s):  
Citric Acid ◽  
Serum Proteins ◽  
Aqueous Media ◽  
Organic Media ◽  
Cell Nuclei ◽  
Intracellular Enzyme ◽  
Cytoplasmic Proteins ◽  
Immunological Tests ◽  
Avian Erythrocytes ◽  
Enzyme Distribution

1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins. 3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation. 4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid. 5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed.

scholarly journals A rapid single step staining technique for DNA analysis by flow microfluorimetry.

1980 ◽  
Vol 28 (9) ◽  
pp. 1021-1024 ◽  
Author(s):  
I W Taylor
Keyword(s):  
Dna Analysis ◽  
Staining Technique ◽  
Single Step ◽  
Cell Nuclei ◽  
Hoechst 33342 ◽  
Monolayer Cultures ◽  
Nonionic Detergent ◽  
Staining Techniques ◽  
Triton X ◽  
Dna Histograms

A new procedure is reported for the staining of DNA, for flow microfluorimetry. It allows the production of stained cell nuclei in a single step by incorporating the DNA stain with a solution of the nonionic detergent Triton-X-100. This method has been found to be applicable to all DNA fluorochromes tested (ethidium bromide, propidium iodide, mithramycin, DAPI, Hoechst 33342). DNA histograms obtained in this way are comparable to those using conventional staining techniques, e.g., ethanol fixation followed by staining. Using this procedure the DNA content distribution of solid tissue or cells from suspension or monolayer cultures can be generated in less than 5 min.

Citric acid extracts a specific set of proteins from isolated cell nuclei

1995 ◽  
Vol 59 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Uwe Rosenberger ◽  
Mehdi Shakibaei ◽  
Christoph Weise ◽  
Peter Franke ◽  
Klaus Buchner
Keyword(s):  
Citric Acid ◽  
Cell Nuclei ◽  
Isolated Cell

Isolation and preservation of cell nuclei for studies on RNA polymerase activity

1970 ◽  
Vol 48 (5) ◽  
pp. 559-565 ◽  
Author(s):  
R. S. D. Read ◽  
C. M. Mauritzen
Keyword(s):  
Rna Polymerase ◽  
Mammalian Cell ◽  
Cell Types ◽  
Polymerase Activity ◽  
Cell Nuclei ◽  
Low Concentrations ◽  
Nonionic Detergent ◽  
Rna Polymerase Activity ◽  
Liver Nuclei ◽  
Nonionic Detergents

The suitability of saponin for the isolation and of glycerol for the preservation of mammalian cell nuclei has been investigated. The nonionic detergent saponin was found to be a useful cell lytic agent in a procedure for the isolation of nuclei from several mammalian cell types. The RNA polymerase activity of rat liver nuclei was not affected by treatment with saponin or with some other nonionic detergents that were tested. Low concentrations of ionic agents also did not affect the activity of the enzyme though higher concentrations caused lysis of the nuclei. The preservation of structure and enzyme activity in the isolated nuclei was achieved by storage at low temperature in a medium containing 70% glycerol together with a suitable concentration of a divalent metal and phosphate buffer.

scholarly journals A METHOD FOR ISOLATING INTACT MITOCHONDRIA AND NUCLEI FROM THE SAME HOMOGENATE, AND THE INFLUENCE OF MITOCHONDRIAL DESTRUCTION ON THE PROPERTIES OF CELL NUCLEI

1955 ◽  
Vol 1 (2) ◽  
pp. 139-153 ◽  
Author(s):  
Alexander L. Dounce ◽  
Robert F. Witter ◽  
Kenneth J. Monty ◽  
Sidney Pate ◽  
Mary A. Cottone
Keyword(s):  
Citric Acid ◽  
Tumor Cells ◽  
Soft Tissues ◽  
Sucrose Solution ◽  
Cell Disruption ◽  
New Method ◽  
Cell Nuclei ◽  
Ph Adjustment ◽  
Glass Homogenizer ◽  
High Degree

1. An improved type of ground glass homogenizer for soft tissues has been described which brings about a high degree of cell disruption and liberation of nuclei without causing appreciable damage to mitochondria. The gentleness and effectiveness of the new homogenizer in respect to isolation of mitochondria have been ascertained by comparing the ATP-ase activities of mitochondria isolated in 0.25 M sucrose solution without pH adjustment using a previous type of homogenizer with those of mitochondria isolated under the same conditions with the aid of the new homogenizer. In these experiments sucrose of 0.25 molarity without pH adjustment has been used in order to maintain the mitochondria in a rather sensitive state so as to make slightly deleterious effects of homogenization readily apparent. 2. A new method is described for the isolation of morphologically intact mitochondria and cell nuclei from the same homogenate. In this procedure the pH of the homogenate in 0.44 M sucrose is maintained at 6.0–6.2 with citric acid during the homogenization. An alternative method employing 0.44 M sucrose plus 0.005 M CaCl2 is given for the isolation of nuclei from tumor cells. However, the latter method does not produce unaltered mitochondria. 3. The α-ketoglutarate, malate, succinate, and hexanoate oxidases of the "intact" mitochondria isolated in 0.44 M sucrose adjusted to pH 6.0–6.2 with very dilute citric acid as described in this paper have been investigated, and it has been shown that the mitochondria compare favorably to those isolated in 0.25 M sucrose by a previously described method. 4. Mitochondria have been found to contain an enzyme which causes nuclei to lose their ability to form gels in dilute alkali. This enzyme is released from the mitochondria when the latter are disrupted. 5. Some properties of nuclei isolated by the new method have been briefly discussed.

Intranuclear Crystals in Regenerating Canine Kidneys

1973 ◽  
Vol 31 ◽  
pp. 412-413
Author(s):  
D.G. Osborne ◽  
L.J. McCormack ◽  
M.O. Magnusson ◽  
W.S. Kiser
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Tubular Epithelial Cell ◽  
Tubular Epithelial Cells ◽  
Cell Nuclei ◽  
Crystalline Structures ◽  
Small Crystals ◽  
Membrane Bound

During a project in which regenerative changes were studied in autotransplanted canine kidneys, intranuclear crystals were seen in a small number of tubular epithelial cells. These crystalline structures were seen in the control specimens and also in regenerating specimens; the main differences being in size and number of them. The control specimens showed a few tubular epithelial cell nuclei almost completely occupied by large crystals that were not membrane bound. Subsequent follow-up biopsies of the same kidneys contained similar intranuclear crystals but of a much smaller size. Some of these nuclei contained several small crystals. The small crystals occurred at one week following transplantation and were seen even four weeks following transplantation. As time passed, the small crystals appeared to fuse to form larger crystals.

Coadministration of citric acid allows oxytetracycline dose reduction in yellowtail Seriola quinqueradiata infected with Vibrio anguillarum

2020 ◽  
Vol 140 ◽  
pp. 25-29
Author(s):  
K Akiyama ◽  
N Hirazawa ◽  
A Hatanaka
Keyword(s):  
Body Weight ◽  
Citric Acid ◽  
Effective Treatment ◽  
Dose Reduction ◽  
Vibrio Anguillarum ◽  
Enhancing Effect ◽  
Bacterial Diseases ◽  
Seriola Quinqueradiata ◽  
Poor Efficacy

Oxytetracycline (OTC) has been commonly used as an effective antibiotic against various fish bacterial diseases, including vibriosis. In this study, the absorption-enhancing effect of citric acid on oral OTC pharmacokinetics and treatment of artificial Vibrio anguillarum infection was evaluated in juvenile yellowtail Seriola quinqueradiata followed by serum OTC concentration analysis. When 25 mg kg-1 body weight (BW) OTC was administered in combination with 1250 mg kg-1 BW citric acid, the serum OTC concentration reached almost the same concentration as that of the group treated with 50 mg kg-1 BW OTC. This coadministration successfully suppressed mortality due to vibriosis similar to the group treated with 50 mg kg-1 BW OTC. Conversely, poor efficacy was observed when only 25 mg kg-1 BW OTC was administered. These results suggest that coadministration of citric acid can be beneficial in reducing the dose of OTC needed for effective treatment, and thus contributes to the goal of reduced use of this antibiotic in aquaculture.

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