Tuning the Hoechst Dye into Color-changing Fluorescent pH Indicator in an Acidic Range

2008 ◽  
Vol 37 (3) ◽  
pp. 246-247
Author(s):  
Shinsuke Sando ◽  
Atsushi Narita ◽  
Masayoshi Hayami ◽  
Yasuhiro Aoyama
Keyword(s):  
Ph Indicator ◽  
Hoechst Dye

Visualizing Preferential Flow Paths using Ammonium Carbonate and a pH Indicator

2002 ◽  
Vol 66 (2) ◽  
pp. 347 ◽  
Author(s):  
Zhi Wang ◽  
Jianhang Lu ◽  
Laosheng Wu ◽  
Thomas Harter ◽  
William A. Jury
Keyword(s):  
Preferential Flow ◽  
Ammonium Carbonate ◽  
Ph Indicator ◽  
Flow Paths ◽  
Preferential Flow Paths

scholarly journals Regression Analysis of Orthogonal, Cylindrical and Multivariable Color Parameters for Colorimetric Surface pH Measurement of Materials

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3682
Author(s):  
Katarína Vizárová ◽  
Izabela Vajová ◽  
Naďa Krivoňáková ◽  
Radko Tiňo ◽  
Zdenko Takáč ◽  
...  
Keyword(s):  
Ph Value ◽  
Critical Factor ◽  
Ph Measurement ◽  
Ph Indicator ◽  
Water Quality Control ◽  
Quality Management Systems ◽  
Surface Ph ◽  
Regression Parameters ◽  
Ph Detection

The surface pH is a critical factor in the quality and longevity of materials and products. Traditional fast colorimetric pH detection-based tests such as water quality control or pregnancy tests, when results are determined by the naked eye, cannot provide quantitative values. Using standard pH papers, paper-printed comparison charts, or colorimetric microfluidic paper-based analytical devices is not suitable for such technological applications and quality management systems (QMSs) where the particular tested material should contain a suitable indicator in situ, in its structure, either before or after the process, the technology or the apparatus that are being tested. This paper describes a method based on the combination of impregnation of a tested material with a pH indicator in situ, its exposure to a process of technology whose impact on pH value is to be tested, colorimetric pH measurement, and approximation of pH value using derived pH characteristic parameters (pH-CPs) based on CIE orthogonal and cylindrical color variables. The hypotheses were experimentally verified using the methyl red pH indicator, impregnating the acid lignin-containing paper, and preparing a calibration sample set with pH in the range 4 to 12 using controlled alkalization. Based on the performed measurements and statistical evaluation, it can be concluded that the best pH-CPs with the highest regression parameters for pH are √∆E, ln (a),√∆H (ab), a/L, h/b and ln (b/a). The experimental results show that the presented method allows a good estimation of pH detection of the material surfaces.

Evaluation of pH indicator-based colorimetric films for ammonia detection using optical waveguides

2009 ◽  
Vol 143 (1) ◽  
pp. 62-70 ◽  
Author(s):  
J. Courbat ◽  
D. Briand ◽  
J. Damon-Lacoste ◽  
J. Wöllenstein ◽  
N.F. de Rooij
Keyword(s):  
Optical Waveguides ◽  
Ph Indicator ◽  
Ammonia Detection

Hybrid Hydrogels for pH Indicator

Silicon ◽  
2021 ◽  
Author(s):  
Nathapong Kertkal ◽  
Pavinee Jinawong ◽  
Appinya Rithiyong ◽  
Boonsri Kusuktham
Keyword(s):  
Ph Indicator ◽  
Hybrid Hydrogels

Using pH Indicator To Demonstrate Supercapacitor Reactions

2019 ◽  
Vol 96 (8) ◽  
pp. 1778-1781
Author(s):  
Emily Traver ◽  
Jamie E. Stark ◽  
Gianna Aleman Milán ◽  
Heather A. Andreas
Keyword(s):  
Ph Indicator

scholarly journals Green Fluorescent Protein as a Noninvasive Intracellular pH Indicator

1998 ◽  
Vol 74 (3) ◽  
pp. 1591-1599 ◽  
Author(s):  
Malea Kneen ◽  
Javier Farinas ◽  
Yuxin Li ◽  
A.S. Verkman
Keyword(s):  
Green Fluorescent Protein ◽  
Intracellular Ph ◽  
Fluorescent Protein ◽  
Ph Indicator ◽  
Green Fluorescent

scholarly journals Enhanced activity of ventricular Na+-HCO3− cotransport in pressure overload hypertrophy

2007 ◽  
Vol 293 (2) ◽  
pp. H1254-H1264 ◽  
Author(s):  
Taku Yamamoto ◽  
Takeshi Shirayama ◽  
Tomohiko Sakatani ◽  
Tomosaburo Takahashi ◽  
Hideo Tanaka ◽  
...  
Keyword(s):  
Ventricular Myocytes ◽  
Single Cells ◽  
Ischemia Reperfusion ◽  
Pressure Overload ◽  
Pcr Analysis ◽  
Mrna Levels ◽  
Aortic Constriction ◽  
Ph Indicator ◽  
Adult Rat ◽  
Acid Extrusion

The Na+-HCO3− cotransporter (NBC) plays a key role in intracellular pH (pHi) regulation in normal ventricular muscle. However, the state of NBC in nonischemic hypertrophied hearts is unresolved. In this study, we examined functional and molecular properties of NBC in adult rat ventricular myocytes. The cells were enzymatically isolated from both normal and hypertrophied hearts. Ventricular hypertrophy was induced by pressure overload created by suprarenal abdominal aortic constriction of 50% for 7 wk. pHi was measured in single cells using the fluorescent pH indicator 2′,7′-bis(2-carboxyethyl)5-( 6 )carboxyfluorescein. Real-time PCR analysis was used to quantitatively assess expression of NBC-encoding mRNA, including SLC4A4 (encoding electrogenic NBC, NBCe1) and SLC4A7 (electroneutral NBC, NBCn1). Our results demonstrate that: 1) mRNA levels of both the electrogenic NBCe1 (SLC4A4) and electroneutral NBCn1 (SLC4A7) forms of NBC were increased by aortic constriction, 2) the onset of NBC upregulation occurred within 3 days after constriction, 3) normal and hypertrophied ventricles displayed regional differences in NBC expression, 4) acid extrusion via NBC ( JNBC) was increased significantly in hypertrophied myocytes, 5) although acid extrusion via Na+/H+ exchange was also increased in hypertrophied myocytes, the relative enhancement of JNBC was larger, 6) membrane depolarization markedly increased JNBC in hypertrophied myocytes, and 7) losartan, an ANG II AT1 receptor antagonist, significantly attenuated the upregulation of both NBCs induced by 3 wk of aortic constriction. Enhanced NBC activity during hypertrophic development provides a mechanism for intracellular Na+ overload, which may render the ventricles more vulnerable to Ca2+ overload during ischemia-reperfusion.

Cellulose Acetate-Based pH Indicator Optodes

1995 ◽  
Vol 48 (6) ◽  
pp. 1081 ◽  
Author(s):  
TJ Cardwell ◽  
RW Cattrall ◽  
LW Deady ◽  
M Dorkos ◽  
AJ Kaye ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Ph Indicator ◽  
Ph Indicators

Some pH indicators for use in optode membranes based on cellulose acetate have been prepared, and applications of membranes containing octyl bis (2,4-dinitrophenyl)acetate in static and flowing systems are discussed.

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